Purification of green fluorescent protein using a two-intein system

Applied Microbiology and Biotechnology

Volumn 77, Issue 5, 2008, Pages 1175-1180

  Zhao, Zhonglin ^a   Lu, Wei ^b   Dun, Baoqing ^b   Jin, Dan ^c   Ping, Shuzhen ^b   Zhang, Wei ^b   Chen, Ming ^b   Xu, Ming Qun ^d   Lin, Min ^b  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c SOUTHWEST UNIVERSITY   (China)

^d NEW ENGLAND BIOLABS   (United States)

Author keywords

Green fluorescent protein; Intein; Protein purification; Protein splicing

Indexed keywords

BACTERIA; BIOASSAY; CELL IMMOBILIZATION; CHITIN; CIRCULAR DICHROISM SPECTROSCOPY; FLUORESCENCE; PURIFICATION;

GREEN FLUORESCENT PROTEIN; INTEINS; PROTEIN PURIFICATION; PROTEIN SPLICING;

PROTEINS;

CHITIN; GREEN FLUORESCENT PROTEIN; INTEIN;

BACTERIUM; BIOASSAY; CHITIN; FLUORESCENCE; MUTATION; PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BACTERIUM CULTURE; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CONFOCAL MICROSCOPY; ESCHERICHIA COLI; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; INTEINS; MUTANT PROTEINS; PROTEIN BINDING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 37449032617     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1233-0     Document Type: Article

References (16)

1. Andersen JB, Sternberg C, Poulsen LK, Bj, rn SP, Givskov M, Molin S (1998) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 64:2240-2246
2. Chong S, Montello GE, Zhang A, Cantor EJ, Liao W, Xu MQ, Benner J (1998) Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res 26:5109-5115
3. Cormack BP, Valdivia RH, Falkow S (1996) FACS optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38
4. David R, Richter MP, Beck-Sickinger AG (2004) Expressed protein ligation method and applications. Eur J Biochem 271:663-677
5. Evans TC, Benner J, Xu MQ (1999a) The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum. J Biol Chem 274:3923-3926
6. Evans TC Jr, Benner J, Xu MQ (1999b) The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins. J Biol Chem 274:18359-18363
7. Guo C, Li Z, Shi Y, Xu M, Wise JG, Trommer WE, Yuan J (2004) Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin. Protein Expr Purif 37:361-367
8. Iwai H, Lingel A, Pl, ckthun A (2001) Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus. J Biol Chem 276:16548-16554
9. Katragadda M, Lambris JD (2006) Expression of compstatin in Escherichia coli: Incorporation of unnatural amino acids enhances its activity. Protein Expr Purif 47:289-295
10. Mathys S, Evans TC Jr, Chute IC, Wu H, Chong S, Benner J, Liu XQ, Xu MQ (1999) Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 231:1-13
11. Muir TW, Sondhi D, Cole PA (1998) Expressed protein ligation: A general method for protein engineering. Proc Natl Acad Sci U S A 95:6705-6710
12. Singleton SF, Simonette RA, Sharma NC, Roca AI (2002) Intein-mediated affinity-fusion purification of the Escherichia coli RecA protein. Protein Expr Purif 26:476-488
13. Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67:509-544
14. Wu W, Wood DW, Belfort G, Derbyshire V, Belfort M (2002) Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing. Nucleic Acids Res 30:4864-4871
15. Xu MQ, Evans TC Jr (2001) Intein-mediated ligation and cyclization of expressed proteins. Methods 24:257-277
16. Xu MQ, Evans TC Jr (2003) Purification of recombinant proteins from E. coli by engineered inteins. Methods Mol Biol 205:43-68