A genetic system yields self-cleaving inteins for bioseparations

Nature Biotechnology

Volumn 17, Issue 9, 1999, Pages 889-892

  Wood, David W ^a,b   Wu, Wei ^a   Belfort, Georges ^b   Derbyshire, Victoria ^a   Belfort, Marlene ^a  

^a STATE UNIVERSITY OF NEW YORK   (United States)

^b Rensselaer Polytechnic Institute   (United States)

Author keywords

Function of conserved residues; Mini intein mutants; PH sensitive cleavage

Indexed keywords

ENDONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; GENETIC ENGINEERING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN ISOLATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; DNA GYRASE; DNA TOPOISOMERASES, TYPE II; ENDOPEPTIDASES; LEUCINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MYCOBACTERIUM; PROTEIN ENGINEERING; PROTEIN PRECURSORS; PROTEIN SPLICING; REC A RECOMBINASES; RECOMBINANT PROTEINS; THYMIDYLATE SYNTHASE; VALINE;

EID: 0032832776     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/12879     Document Type: Article

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