Oligomerized pool engineering (OPEN): An 'open-source' protocol for making customized zinc-finger arrays

Nature Protocols

Volumn 4, Issue 10, 2009, Pages 1471-1501

  Maeder, Morgan L ^a   Thibodeau Beganny, Stacey ^a   Sander, Jeffry D ^c   Voytas, Daniel F ^c,d   Joung, J Keith ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c IOWA STATE UNIVERSITY   (United States)

^d UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; ZINC FINGER PROTEIN;

ARTICLE; BINDING SITE; DNA BINDING; DNA PURIFICATION; EXPRESSION VECTOR; GENE CONSTRUCT; GENETIC ENGINEERING; INTERMETHOD COMPARISON; INTERNET; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN FUNCTION; REPORTER GENE; TWO HYBRID SYSTEM; WEB BROWSER;

BACTERIOLOGICAL TECHNIQUES; BACTERIOPHAGES; BINDING SITES; ESCHERICHIA COLI; GENE LIBRARY; GENOMICS; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINATION, GENETIC; SOFTWARE; ZINC FINGERS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 70349146995     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.98     Document Type: Article

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