Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage

Biochemical and Biophysical Research Communications

Volumn 334, Issue 4, 2005, Pages 1191-1197

  Mani, Mala ^a   Smith, Jeff ^b,c   Kandavelou, Karthikeyan ^a   Berg, Jeremy M ^b,d   Chandrasegaran, Srinivasan ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c DUKE UNIVERSITY MEDICAL CENTER   (United States)

^d NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES   (United States)

Author keywords

Chimeric nucleases; Directed mutagenesis; Gene targeting; Gene therapy; Homologous recombination; Homology directed repair; Non homologous end joining; Protein engineering; Sequence specific cleavage; Zinc finger nucleases

Indexed keywords

BIOTIN; DIVALENT CATION; DOUBLE STRANDED DNA; METAL ION; MONOMER; NUCLEASE; PROTEIN FOKI; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ARTICLE; BINDING SITE; CONCENTRATION (PARAMETERS); DIMERIZATION; DNA BINDING; DNA CLEAVAGE; GENE TARGETING; GENE THERAPY; HOMOLOGOUS RECOMBINATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN ENGINEERING;

BINDING SITES; DEOXYRIBONUCLEASES; DNA; DNA DAMAGE; DNA-BINDING PROTEINS; ENZYME ACTIVATION; PROTEIN BINDING; ZINC FINGERS;

EID: 23044460704     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.07.021     Document Type: Article

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