Engineering polydactyl zinc-finger transcription factors

Nature Biotechnology

Volumn 20, Issue 2, 2002, Pages 135-141

  Beerli, Roger R ^a,b   Barbas, Carlos F ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b CYTOS BIOTECHNOLOGY AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PROTEINS; ZINC;

GENE FUNCTIONS;

BIOTECHNOLOGY;

DNA; GENE PRODUCT; TRANSCRIPTION FACTOR; ZINC FINGER PROTEIN;

DNA BINDING; DNA SEQUENCE; GENE ACTIVATION; GENETIC ENGINEERING; GENETIC TRANSCRIPTION; HUMAN; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVIEW; TRANSCRIPTION REGULATION;

ANIMALS; BINDING SITES; DNA; GENE EXPRESSION REGULATION; HUMANS; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; ZINC FINGERS;

ANIMALIA;

EID: 0036168937     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0202-135     Document Type: Review

References (60)

1. Control of gene transcription: An outline
2. Different activation domains stimulate transcription from remote ("enhancer") and proximal ("promoter") positions
3. Active repression mechanisms of eukaryotic transcription repressors
4. Expressing the human genome
5. The sequencing of the human genome
6. Initial sequencing and analysis of the human genome
7. Zinc finger-DNA recognition: Crystal structure of a Zif268-DNA complex at 2.1 Å
8. Design of novel sequence-specific DNA-binding proteins
9. Zif268 protein-DNA complex refined at 1.6 A: A model system for understanding zinc finger-DNA interactions
10. Toward rules relating zinc finger protein sequences and DNA binding site preferences
11. Zinc finger-DNA recognition: Analysis of base specificity by site-directed mutagenesis
12. Designing zinc-finger ADR1 mutants with altered specificity of DNA binding to T in UAS1 sequences
13. Zinc finger phage: Affinity selection of fingers with new DNA-binding specificities
14. Toward a code for the interactions of zinc fingers with DNA: Selection of randomized fingers displayed on phage
15. In vitro selection of zinc fingers with altered DNA-binding specificity
16. Building zinc fingers by selection: Toward a therapeutic application
17. A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
18. Toward controlling gene expression at will: Selection and design of zinc finger domains recognizing each of the 5′-GNN- 3′ DNA target sequences
19. Insights into the molecular recognition of the 3′-GNN-3′ family of DNA sequences by zinc-finger domains
20. Development of zinc finger domains for recognition of the 5′-ANN- 3′ family of DNA sequences and their use in the construction of artificial transcription factors
21. Combinatorial immunoglobulin libraries on the surface of phage (Phabs): Rapid selection of antigen-specific Fabs
22. Design of polydactyl zinc-finger proteins for unique addressing within complex genomes
23. Chemically regulated zinc finger transcription factors
24. Combining structure-based design with phage display to create new Cys(2)His(2) zinc finger dimers
25. Dimerization of zinc fingers mediated by peptides evolved in vitro from random sequences
26. Positive and negative regulation of endogenous genes by designed transcription factors
27. Getting a handhold on DNA: Design of poly-zinc finger proteins with femtomolar dissociation constants
28. Improved DNA-binding specificity from polyzinc finger peptides by using strings of two-finger units
29. Quantifying DNA-protein interactions by double-stranded DNA arrays
30. Exploring the DNA-binding specificities of zinc fingers with DNA microarrays
31. Target Detection Assay (TDA): A versatile procedure to determine DNA binding sites as demonstrated on SP1 protein
32. Design of polyzinc finger peptides with structured linkers
33. A 2.2 Å resolution crystal structure of a designed zinc finger protein bound to DNA
34. Synergy between adjacent zinc fingers in sequence-specific DNA recognition
35. Toward controlling gene expression at will: Specific regulation of the erbB-2/HER- 2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks
36. Comprehensive DNA recognition through concerted interactions from adjacent zinc fingers
37. Analysis of zinc fingers optimized via phage display: Evaluating the utility of a recognition code
38. Beyond the "recognition code": Structures of two Cys2His2 zinc finger/TATA box complexes
39. A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter
40. In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities
41. A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions
42. A genetic screen identifies a cellular regulator of adeno-associated virus
43. Molecular dissection of DNA sequences and factors involved in slow muscle-specific transcription
44. Krüppel-associated boxes are potent transcriptional repression domains
45. A complex containing N-CoR, mSin 3 and histone deacetylase mediates transcriptional repression
46. In vivo repression by a site specific DNA binding protein designed against an oncogenic sequence
47. Transcriptional repression by zinc finger peptides. Exploring the potential for applications in gene therapy
48. Zinc finger proteins as designer transcription factors
49. Design of TATA box-binding protein/zinc finger fusions for targeted regulation of gene expression
50. Transcriptional activation by recruitment
51. Regulation of an endogenous locus using a panel of designed zinc finger proteins targeted to accessible chromatin regions
52. Synthetic zinc finger transcription factor action at an endogenous chromosomal site
53. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
54. A regulatory system for use in gene transfer
55. Ecdysone-inducible gene expression in mammalian cells and transgenic mice
56. Chimeric receptors as gene switches
57. A versatile framework for the design of ligand-dependent, transgene-specific transcription factors
58. Meeting report: The new biology: Genomics fosters a systems approach and collaborations between academic, government, and industry scientists
59. Stimulation of homologous recombination through targeted cleavage by a chimeric nuclease