Enhancing gene targeting with designed zinc finger nucleases

Science

Volumn 300, Issue 5620, 2003, Pages 764-

  Bibikova, Marina ^a,b   Beumer, Kelly ^a   Trautman, Jonathan K ^a   Carroll, Dana ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b ILLUMINA INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE;

GENE; TARGETING;

DNA CLEAVAGE; DROSOPHILA MELANOGASTER; GENE EXPRESSION; GENE TARGETING; GENETIC RECOMBINATION; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE HOMOLOGY; SHORT SURVEY; TRANSGENE; ZINC FINGER MOTIF;

ANIMALS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DIMERIZATION; DNA NUCLEOTIDYLTRANSFERASES; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ENDODEOXYRIBONUCLEASES; FEMALE; GENE TARGETING; GENES, INSECT; GENETIC VECTORS; GERM-LINE MUTATION; INSECT PROTEINS; MALE; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; X CHROMOSOME; ZINC FINGERS;

DROSOPHILA MELANOGASTER; MELANOGASTER;

EID: 0037510038     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1079512     Document Type: Short Survey

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17. We are extremely grateful to K. Golic and his group, particularly M. Golic, Y. Rong, and W. Gong, for fly stocks, the donor vector, hospitality, communication of results in advance of publication, and advice. We thank D. Segal and C. Barbas for providing the zinc finger coding sequences from which the yA and yB combinations were constructed. J. Staton contributed to the analysis of the germline y mutants. K.B. was supported by a postdoctoral fellowship from the NIH, and the work was made possible by a research grant from the National Institute of General Medical Sciences to D.C. (GM58504). Partial support was also provided by a center support grant from the National Cancer Institute.