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Propidium iodide stained cells that have survived a washing step could have internal damage that leads to a long-term (overnight) effect of a particular compound that has entered the cell. This common toxicity measure merely describes the amount of damage to cells that die as the transfection reagents enter the cell. After overnight incubation these cells are no longer viable, and would be washed away. To account for this, we measured the total count of cells per unit per well
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Propidium iodide stained cells that have survived a washing step could have internal damage that leads to a "long-term" (overnight) effect of a particular compound that has entered the cell. This common toxicity measure merely describes the amount of damage to cells that die as the transfection reagents enter the cell. After overnight incubation these cells are no longer viable, and would be washed away. To account for this, we measured the total count of cells per unit per well.
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