RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions

Nature Protocols

Volumn 1, Issue 3, 2006, Pages 1617-1620

  Lehner, Ben ^a,b   Tischler, Julia ^a   Fraser, Andrew G ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^b CENTRE FOR GENOMIC REGULATION CRG   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ARTICLE; CAENORHABDITIS ELEGANS; GENETIC EPISTASIS; GENETIC PROCEDURES; GENETICS; RNA INTERFERENCE;

ANIMALS; CAENORHABDITIS ELEGANS; EPISTASIS, GENETIC; GENETIC TECHNIQUES; RNA INTERFERENCE;

EID: 34249740079     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.245     Document Type: Article

References (12)

1. Timmons, L. & Fire, A. Specific interference by ingested dsRNA. Nature 395, 854 (1998).
2. Kamath, R.S. et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421, 231-237 (2003).
3. Lehner, B., Fraser, A.G. & Sanderson, C.M. Technique review: How to use RNA interference. Brief Funct. Genomic Proteomic 3, 68-83 (2004).
4. Lehner, B., Crombie, C., Tischler, J., Fortunato, A. & Fraser, A.G. Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways. Nat. Genet. 38, 896-903 (2006).
5. van Haaften, G., Vastenhouw, N.L., Nollen, E.A., Plasterk, R.H. & Tijsterman, M. Gene interactions in the DNA damage-response pathway identified by genome-wide RNA-interference analysis of synthetic lethality. Proc Natl. Acad. Sci. USA 101, 12992-12996 (2004).
6. Nollen, E.A. et al. Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Proc. Natl. Acad. Sci. USA 101, 6403-5408 (2004).
7. Ahringer, J. (ed.) Reverse genetics. in WormBook (ed. The C. elegans Research Community) (http://www.wormbook.org, 2006).
8. Tischler, J., Lehner, B., Chen, N. & Fraser, A.G. Combinatorial RNA interference in C. elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution. Genome Biol, 7, R69 (2006).
9. Simmer, F. et al. Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr. Biol. 12, 1317-1319 (2002).
10. Kennedy, S., Wang, D. & Ruvkun, G. A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature 427 645-649 (2004).
11. Wang, D. et al. Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants. Nature 436, 593-597 (2005).
12. Lehner, B. et al. Loss of LIN-35, the Caenorhabditis elegans of the tumor suppressor p105Rb, results in enhanced RNA interference. Genome Biol. 7, R4 (2006).