Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans

Nature

Volumn 434, Issue 7032, 2005, Pages 462-469

  Sonnichsen B ^a   Koski, L B ^a,f   Walsh, A ^a   Marschall, P ^a,g   Neumann, B ^a,h   Brehm, M ^a   Alleaume, A M ^a,i   Artelt, J ^a,j   Bettencourt, P ^a,k   Cassin, E ^b,l   Hewitson, M ^a   Holz, C ^a   Khan, M ^a   Lazik, S ^a   Martin, C ^a   Nitzsche, B ^a,m   Ruer, M ^b   Stamford, J ^b   Winzi, M ^a   Heinkel, R ^a,m   Roder M ^a,n   Finell, J ^a,m   Hantsch H ^a   Jones, S J M ^c   Jones, M ^d,o   Piano, F ^e   Gunsalus, K C ^e   Oegema, K ^b,p   Gonczy P ^b,q   Coulson, A ^d,r   Hyman, A A ^b   Echeverri, C J ^a   more..

^a Cenix BioScience GmbH   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^c BRITISH COLUMBIA CANCER RESEARCH CENTRE   (Canada)

^d WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^e NEW YORK UNIVERSITY   (United States)

^f Universite de Montreal   (Canada)

^g MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

^h EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

ⁱ INSTITUT COCHIN   (France)

^j DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^k INSTITUTO GULBENKIAN DE CIÊNCIA   (Portugal)

^l SAN LUIGI GONZAGA HOSPITAL   (Italy)

^m Ingeniweb ^*  (Finland)

ⁿ Ingeniweb   (France)

^o SIMON FRASER UNIVERSITY   (Canada)

^p UNIVERSITY OF CALIFORNIA   (United States)

^q SWISS INST FOR EXP CANCER RESEARCH   (Switzerland)

^r MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

more..

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CELLS; DATA REDUCTION; GENES; LARGE SCALE SYSTEMS; MICROSCOPIC EXAMINATION;

BIOCHEMICAL PATHWAYS; CELLULAR PROCESSES; EMBRYOGENESIS; PHENOTYPIC DATA SETS;

RNA;

DNA; MESSENGER RNA; RNA; SMALL INTERFERING RNA; CAENORHABDITIS ELEGANS PROTEIN; HELMINTH RNA;

GENOME;

ARTICLE; CAENORHABDITIS ELEGANS; CELL CYCLE; CELL DIVISION; DNA REPLICATION; EMBRYO; EMBRYO DEVELOPMENT; FUNCTIONAL GENOMICS; GENE; GENE SILENCING; GENOME; MICROSCOPY; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA INTERFERENCE; ANIMAL; BIOLOGY; GENETICS; GENOMICS; METABOLISM; PHYSIOLOGY; PRENATAL DEVELOPMENT;

ANIMALS; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; COMPUTATIONAL BIOLOGY; EMBRYONIC DEVELOPMENT; GENES, HELMINTH; GENOME; GENOMICS; PHENOTYPE; RNA INTERFERENCE; RNA, HELMINTH; RNA, MESSENGER;

EID: 15844431376     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature03353     Document Type: Article

References (21)

1. Lum, L. et al. Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells. Science 299, 2039-2045 (2003).
2. Gönczy, P. et al. Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature 408, 331-336 (2000).
3. Kamath, R. S. et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421, 231-237 (2003).
4. Clemens, J. C. et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl Acad. Sci. USA 97, 6499-6503 (2000).
5. Kiger, A. et al. A functional genomic analysis of cell morphology using RNA interference. J. Biol. 2, 27.1-27.15 (2003).
6. Maeda, I. et al. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr. Biol. 11, 171-176 (2001).
7. Piano, F. et al. Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans. Curr. Biol. 12, 1959-1964 (2002).
8. Walhout, A. J. et al. Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline. Curr. Biol. 12, 1952-1958 (2002).
9. Schneider, S. Q. & Bowerman, B. Cell polarity and the cytoskeleton in the Caenorhabditis elegans zygote. Annu. Rev. Genet. 37, 221-249 (2003).
10. Gönczy, P. et al. Dissection of cell division processes in the one cell stage Caenorhabditis elegans embryo by mutational analysis. J. Cell Biol. 144, 927-946 (1999).
11. Strome, S. & Wood, W. B. Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos. Cell 35, 15-25 (1983).
12. Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806-811 (1998).
13. The C. elegans Sequencing Consortium. Genome sequence of the nematode C. elegans: a platform for investigating biology. Science 282, 2012-2018 (1998).
14. Parrish, S. et al. Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference. Mol. Cell 6, 1077-1087 (2000).
15. Zipperlen, P. et al. Roles for 147 embryonic lethal genes on C. elegans chromosome I identified by RNA interference and video microscopy. EMBO J. 20, 3984-3992 (2001).
16. Askjaer, P. et al. Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos. Mol. Biol. Cell 13, 4355-4370 (2002).
17. Gönczy, P. et al. Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo. J. Cell Biol, 147, 135-150 (1999).
18. Davy, A. et al. A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome. EMBO Rep. 2, 821-828 (2001).
19. Giaever, G. et al. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418, 387-391 (2002).
20. Brenner, S. The genetics of Caenorhabditis elegans. Genetics 77, 71-94 (1974).
21. Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402 (1997).