RNA interference

Nature

Volumn 418, Issue 6894, 2002, Pages 244-251

  Hannon, Gregory J ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENETIC ENGINEERING; NUCLEIC ACIDS;

GENE SILENCING;

RNA;

DOUBLE STRANDED RNA; NUCLEIC ACID;

GENE; RNA;

CONTROLLED STUDY; GENE CONTROL; GENE EXPRESSION; GENE FUNCTION; GENE SILENCING; GENETIC TRANSCRIPTION; GENOME; NONHUMAN; PARASITE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVIEW;

ANIMALS; GENE SILENCING; GENETIC TECHNIQUES; GENOME; MODELS, GENETIC; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, DOUBLE-STRANDED;

EID: 0037062951     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/418244a     Document Type: Review

References (100)

1. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
2. par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed
3. Specific interference by ingested dsRNA
4. Inhibition of lower pigmentation by antisense CHS genes: Promoter and minimal sequence requirements for the antisense effect
5. Introduction of a chimeric chalcone synthetase gene in Petunia results in reversible cosuppression of homologous genes in trans
6. Altered gene expression in plants due to trans interactions between homologous genes
7. Chalcone synthase cosuppression phenotypes in petunia flowers: Comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences
8. Single copies of a strongly expressed 35S-driven transgene undergo posttranscriptional silencing
9. The frequency and degree of cosuppression by sense chalcone synthease transgenes are dependent on promoter strength and are reduced by premature nonsense codons in the transgene coding sequence
10. Initiation and maintenance of virus-induced gene silencing
11. Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA
12. RNA-mediated virus resistance in transgenic plants: Exploitation of a cellular pathway possibly involved in RNA degradation
13. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA
14. The rest is silence
15. Quelling: Transient inactivation of gene expression in Neurospora crassa by transformation with homologous sequences
16. Production of antisense RNA leads to effective and specific inhibition of gene expression in C. elegans muscle
17. Transgene-mediated cosuppression in the C. elegans germ line
18. Cosuppression in Drosophila: Gene silencing of Alcohol dehydrogenase by white-Adh transgenes is Polycomb dependent
19. An RNA-dependent RNA polymerase gene in Arabidopsis is required for post-transcriptional gene silencing mediated by a transgene but not by a virus
20. Suppression of beta-1,3-glucanase transgene expression in homozygous plants
21. RNA-directed transcriptional gene silencing in plants can be inherited independently of the RNA trigger and requires Met1 for maintenance
22. RNA-directed de novo methylation of genomic sequences in plants
23. Transcriptional silencing and promoter methylation triggered by double-stranded RNA
24. RNAi related mechanisms affect both transcriptional and post-transcriptional transgene silencing in Drosophila
25. The rde-1 gene, RNA interference, and transposon silencing in C. elegans
26. Using RNA interference to identify genes required for RNA interference
27. Post-transcriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C elegans
28. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
29. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway
30. Targeted mRNA degradation by double-stranded RNA in vitro
31. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
32. A species of small antisense RNA in posttranscriptional gene silencing in plants
33. RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
34. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate
35. A novel type of RNase III family proteins in eukaryotes
36. Role for a bidentate ribonuclease in the initiation step of RNA interference
37. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
38. A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans
39. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing
40. Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage
41. ATP requirements and small interfering RNA structure in the RNA interference pathway
42. Argonaute 2, a link between genetic and biochemical analyses of RNAi
43. AGO1 defines a novel locus of Arabidopsis controlling leaf development
44. Post-transcriptional gene silencing by double-stranded RNA
45. Genetic requirements for inheritance of RNAi in C. elegans
46. mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD
47. Distinct roles for RDE-1 and RDE-4 during RNA interference in Caenorhabditis elegans
48. Involvement of small RNAs and role of the qde genes in the gene silencing pathway in Neurospora
49. Systemic acquired silencing: Transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions
50. On the role of RNA amplification in dsRNA-triggered gene silencing
51. Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato
52. Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance
53. Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase
54. EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans
55. RNAi as random degradative PCR: siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs
56. Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA dependent RNA polymerase
57. Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA
58. HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal
59. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-I
60. Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase
61. Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase
62. RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAs
63. A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans
64. DNA methylation and epigenetic inheritance in plants and filamentous fungi
65. Gene silencing and homology-dependent gene silencing in Arabidopsis: Genetic modifiers and DNA methylation
66. AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals
67. A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana
68. Viruses and gene silencing in plants
69. Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems
70. Developmental roles and molecular characterization of a Drosophila homologue of Arabidopsis Argonautel, the founder of a novel gene superfamily
71. A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal
72. Fertile hypomorphic ARGONAUTE (ago1) mutants impaired in post-transcriptional gene silencing and virus resistance
73. Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis
74. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
75. Ambros elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
76. The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation
77. The lin-4RBCC gene acts in the C. elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor
78. A bulged lin-4/lin-14 RNA duplex is sufficient for Caenorhabditis elegans lin-14 temporal gradient formation
79. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
80. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
81. Identification of novel genes coding for small expressed RNAs
82. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
83. An extensive class of small RNAs in Caenorhabditis elegans
84. miRNPs: A novel class of ribonucleoproteins containing numerous microRNAs
85. Micro RNAs are complementary to 3′ UTR sequence motifs that mediate negative post-transcriptional regulation
86. Specific interference with gene function by double-stranded RNA in early mouse development
87. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference
88. Induction of apoptosis by the dsRNA-dependent protein kinase (PKR): Mechanism of action
89. Interactions between the double-stranded RNA binding motif and RNA: Definition of the binding site for the interferon-induced protein kinase DAI (PKR) on adenovirus VA RNA
90. Total silencing by intron-spliced hairpin RNAs
91. Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes
92. Heritable gene silencing in Drosophila using double-stranded RNA
93. Double-stranded RNA interference in Trypanosoma brucei using head-to-head promoters
94. Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA
95. Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters
96. Stable suppression of gene expression by RNAi in mammalian cells
97. A system for stable expression of short interfering RNAs in mammalian cells
98. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells
99. Development of an inducible pol III transcription system essentially requiring a mutated form of the TATA-binding protein
100. Control of the functional activity of an antisense RNA by a tetracycline-responsive derivative of the human U6 snRNA promoter