Erratum: Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure, (Cellular and Molecular Life Sciences CMLS, (2006), 63, 10, (1149-1164), 10.1007/s00018-005-5557-6);Recombinant G protein-coupled receptors from expression to renaturation: A challenge towards structure

Cellular and Molecular Life Sciences

Volumn 63, Issue 10, 2006, Pages 1149-1164

  Sarramegna, V ^a,b   Muller, I ^a   Milon, A ^a   Talmont, F ^a  

^a INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^b UNIVERSITÉ DE LA NOUVELLE CALÉDONIE   (New Caledonia)

Author keywords

GPCR; Membrane protein; Purification; Recombinant; Solubilization; Structure

Indexed keywords

G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; RECOMBINANT RECEPTOR; RHODOPSIN;

BIOLOGY; DRUG MECHANISM; DRUG TARGETING; HUMAN; MOLECULAR RECOGNITION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RECEPTOR SOLUBILIZATION; RENATURATION; REVIEW; SIGNAL TRANSDUCTION; THREE DIMENSIONAL IMAGING;

BOS TAURUS;

EID: 33745293916     PISSN: 1420682X     EISSN: 15691632     Source Type: Journal    
DOI: 10.1007/s00018-006-0001-0     Document Type: Erratum

References (108)

1. Bockaert J. and Pin J. P. (1999) Molecular tinkering of G protein-coupled receptors: an evolutionary success. EMBO J. 18: 1723-1729
2. Thomsen W. J., Gatlin J., Unett D. J., and Behan P. B. (2004) Developing functional GPCR screens. www.currentdrugdiscovery.com January 2004, pp. 13-18
3. Palczewski K., Kumasaka T., Hori T., Behnke C. A., Motoshima H., Fox B. A. et al. (2000) Crystal structure of rhodopsin: G protein-coupled receptor. Science 289: 739-745
4. Sarramegna V., Talmont F., Demange P. and Milon A. (2003) Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification. Cell. Mol. Life Sci. 60: 1529-1546
5. Inooka H., Ohtaki T., Kitahara O., Ikegami T., Endo S., Kitada C. et al. (2001) Conformation of a peptide ligand bound to its G-protein coupled receptor. Nat. Struct. Biol. 8: 161-165
6. Kunishima N., Shimada Y., Tsuji Y., Sato T., Yamamoto M., Kumasaka et al. (2000) Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor. Nature 407: 971-977
7. Furukawa H., Hamada T., Hayashi M. K., Haga T., Muto Y., Hirota H. et al. (2002) Conformation of ligand bound to the muscarinic acetylcholine receptor. Mol. Pharmacol. 62: 778-787
8. Luca S., White J. F., Sohal A. K., Filippov D. V., Boom J. H. van, Grisshammer R. et al. (2003) The conformation of neurotensin bound to its G protein-coupled receptor. Proc. Natl. Acad. Sci. USA 100: 10706-10711
9. Kiefer H., Krieger J., Olszewski J. D., Von Heijne G., Prestwich G. D. and Breer H. (1996) Expression of an olfactory receptor in Escherichia coli: purification reconstitution and ligand binding. Biochemistry 35: 16077-16084
10. Kiefer H., Maier K. and Vogel R. (1999) Refolding of G-protein-coupled receptors from inclusion bodies produced in Escherichia coli. Biochem. Soc. Trans. 27: 908-912
11. Baneres J. L. and Parello J. (2003) Structure-based analysis of GPCR function: evidence for a novel pentameric assembly between the dimeric leukotriene B4 receptor BLT1 and the G-protein. J. Mol. Biol. 329: 815-829
12. Baneres J. L., Martin A., Hullot P., Girard J. P., Rossi J. C. and Parello J. (2003) Structure-based analysis of GPCR function: conformational adaptation of both agonist and receptor upon leukotriene B4 binding to recombinant BLT1. J. Mol. Biol. 329: 801-814
13. Baneres J. L., Mesnier D., Martin A., Joubert L., Dumuis A. and Bockaert J. (2005) Molecular characterization of a purified 5-HT4 receptor: a structural basis for drug efficacy. J. Biol. Chem. 280: 20253-20260
14. Carrio M. M. and Villaverde A. (2002) Construction and deconstruction of bacterial inclusion bodies. J. Biotechnol. 96: 3-12
15. Maire M. le, Champeil P. and Moller J. V. (2000) Interaction of membrane proteins and lipids with solubilizing detergents. Biochim. Biophys. Acta 1508: 86-111
16. Seddon A. M., Curnow P. and Booth P. J. (2004) Membrane proteins lipids and detergents: not just a soap opera. Biochim. Biophys. Acta 1666: 105-117
17. Helenius A. and Simons K. (1975) Solubilization of membranes by detergents. Biochim. Biophys. Acta 415: 29-79
18. Helenius A., McCaslin D. R., Fries E. and Tanford C. (1979) Properties of detergents. Methods Enzymol. 56: 734-749
19. Volkin D. B., Mach H. and Middaugh C. R. (1997) Degradative covalent reactions important to protein stability. Mol. Biotechnol. 8: 105-122
20. Lau F. W. and Bowie J. U. (1997) A method for assessing the stability of a membrane protein. Biochemistry 36: 5884-5892
21. Paulsen H., Finkenzeller B. and Kuhlein N. (1993) Pigments induce folding of light-harvesting chlorophyll a/b-binding protein. Eur. J. Biochem. 215: 809-816
22. Booth P. J. and Paulsen H. (1996) Assembly of light-harvesting chlorophyll a/b complex in vitro: time-resolved fluorescence measurements. Biochemistry 35: 5103-5108
23. Booth P. J., Flitsch S. L., Stern L. J., Greenhalgh D. A., Kim P. S. and Khorana H. G. (1995) Intermediates in the folding of the membrane protein bacteriorhodopsin. Nat. Struct. Biol. 2: 139-143
24. Levy D., Bluzat A., Seigneuret M. and Rigaud J. L. (1990) A systematic study of liposome and proteoliposome reconstitution involving Bio-Bead-mediated Triton X-100 removal. Biochim. Biophys. Acta 1025: 179-190
25. Mazina K. E., Strader C. D., Tota M. R., Daniel S. and Fong T. M. (1996) Purification and reconstitution of a recombinant human neurokinin-1 receptor. J. Recept. Signal. Transduct. Res. 16: 191-207
26. Jong L. A. de, Grunewald S., Franke J. P., Uges D. R. and Bischoff R. (2004) Purification and characterization of the recombinant human dopamine D2S receptor from Pichia pastoris. Protein Expr. Purif. 33: 176-184
27. Nekrasova E., Sosinskaya A., Natochin M., Lancet D., and Gat U. (1996) Overexpression solubilization and purification of rat and human olfactory receptors. Eur. J. Biochem. 238: 28-37
28. Ratnala V. R., Swarts H. G., VanOostrum J., Leurs R. DeGroot H. J., Bakker R. A. et al. (2004) Large-scale overproduction functional purification and ligand affinities of the His-tagged human histamine H1 receptor. Eur. J. Biochem. 271: 2636-2646
29. Schiller H., Molsberger E., Janssen P., Michel H. and Reilander H. (2001) Solubilization and purification of the human ETB endothelin receptor produced by high-level fermentation in Pichia pastoris. Receptors Channels 7: 453-469
30. Sarramegna V., Muller I., Mousseau G., Froment C., Monsarrat B., Milon A. et al. (2005) Solubilization purification and mass spectrometry analysis of the human mu-opioid receptor expressed in Pichia pastoris. Protein Expr. Purif. 43: 85-93
31. Sarramegna V., Talmont F., Seree de Roch M., Milon A. and Demange P. (2002) Green fluorescent protein as a reporter of human mu-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris. J Biotechnol 99: 23-39
32. Sarramegna V., Demange P., Milon A. and Talmont F. (2002) Optimizing functional versus total expression of the human mu-opioid receptor in Pichia pastoris. Protein Expr. Purif. 24: 212-220
33. Mirzabekov T., Bannert N., Farzan M., Hofmann W., Kolchinsky P., Wu L. et al. (1999) Enhanced expression native purification and characterization of CCR5 a principal HIV-1 coreceptor. J. Biol. Chem. 274: 28745-28750
34. Jones M. N. (1999) Surfactants in membrane solubilisation. Int. J. Pharm. 177: 137-159
35. Neugebauer J. M. (1990) Detergents: an overview. Methods Enzymol. 182: 239-253
36. Babcock G. J., Mirzabekov T., Wojtowicz W. and Sodroski J. (2001) Ligand binding characteristics of CXCR4 incorporated into paramagnetic proteoliposomes. J. Biol. Chem. 276: 38433-38440
37. Staudinger R. and Bandres J. C. (2000) Solubilization of the chemokine receptor CXCR4. Biochem. Biophys. Res. Commun. 274: 153-156
38. Tucker J. and Grisshammer R. (1996) Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem. J. 317: 891-899
39. Liitti S., Matikainen M. T., Scheinin M., Glumoff T. and Goldman A. (2001) Immunoaffinity purification and reconstitution of human alpha(2)-adrenergic receptor subtype C2 into phospholipid vesicles. Protein Expr. Purif. 22: 1-10
40. Vasudevan S., Hulme E. C., Bach M., Haase W., Pavia J. and Reilander H. (1995) Characterization of the rat m3 muscarinic acetylcholine receptor produced in insect cells infected with recombinant baculovirus. Eur. J. Biochem. 227: 466-475
41. Rinken A., Kameyama K., Haga T. and Engstrom L. (1994) Solubilization of muscarinic receptor subtypes from baculovirus infected Sf9 insect cells. Biochem. Pharmacol. 48: 1245-1251
42. Weiss H. M. and Grisshammer R. (2002) Purification and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli. Eur. J. Biochem. 269: 82-92
43. Park P. S. and Wells J. W. (2003) Monomers and oligomers of the M2 muscarinic cholinergic receptor purified from Sf9 cells. Biochemistry 42: 12960-12971
44. Bodor E. T., Waldo G. L., Hooks S. B., Corbitt J., Boyer J. L. and Harden T. K. (2003) Purification and functional reconstitution of the human P2Y12 receptor. Mol. Pharmacol. 64: 1210-1216
45. Dann C. E., Hsieh J. C., Rattner A., Sharma D., Nathans J. and Leahy D. J. (2001) Insights into Wnt binding and signalling from the structures of two Frizzled cysteine-rich domains. Nature 412: 86-90
46. Parker E. M., Kameyama K., Higashijima T. and Ross E. M. (1991) Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells. J. Biol. Chem. 266: 519-527
47. Christoffers K. H. Li H. and Howells R. D. (2005) Purification and mass spectrometric analysis of the delta opioid receptor. Brain Res. Mol. Brain Res. 136: 54-64
48. Christoffers K. H., Li H., Keenan S. M. and Howells R. D. (2003) Purification and mass spectrometric analysis of the mu opioid receptor. Brain Res. Mol. Brain Res. 118: 119-131
49. Reilander H., Boege F., Vasudevan S., Maul G., Hekman M., Dees C. et al. (1991) Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells. FEBS. Lett. 282: 441-444
50. Cummings R. D. and Kornfeld S. (1982) Fractionation of asparagine-linked oligosaccharides by serial lectin-agarose affinity chromatography: a rapid sensitive and specific technique. J. Biol. Chem. 257: 11235-11240
51. Ohtaki T., Ogi K., Masuda Y., Mitsuoka K., Fujiyoshi Y., Kitada C. et al. (1998) Expression purification and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide: large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells. J. Biol. Chem. 273: 15464-15473
52. Hochuli E. (1988) Large-scale chromatography of recombinant proteins. J. Chromatogr. 444: 293-302
53. Janknecht R., Martynoff G. de, Lou J., Hipskind R. A., Nordheim A. and Stunnenberg H. G. (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc. Natl. Acad. Sci. USA 88: 8972-8976
54. Flachmann R. and Kuhlbrandt W. (1996) Crystallization and identification of an assembly defect of recombinant antenna complexes produced in transgenic tobacco plants. Proc. Natl. Acad. Sci. USA 93: 14966-14971
55. Janssen J. J., Bovee-Geurts P. H., Merkx M. and DeGrip W. J. (1995) Histidine tagging both allows convenient single-step purification of bovine rhodopsin and exerts ionic strength-dependent effects on its photochemistry. J. Biol. Chem. 270: 11222-11229
56. Krebs M. P., Spudich E. N. and Spudich J. L. (1995) Rapid high-yield purification and liposome reconstitution of polyhistidine-tagged sensory rhodopsin I. Protein Expr. Purif. 6: 780-788
57. + sugar symport is catalyzed by a single polypeptide. Biochemistry 34: 4412-4420
58. Grisshammer R. and Tucker J. (1997) Quantitative evaluation of neurotensin receptor purification by immobilized metal affinity chromatography. Protein Expr. Purif. 11: 53-60
59. Pawate S., Schey K. L., Meier G. P., Ullian M. E., Mais D. E. and Halushka P. V. (1998) Expression characterization and purification of C-terminally hexahistidine-tagged thromboxane A2 receptors. J. Biol. Chem. 273: 22753-22760
60. David N. E., Gee M., Andersen B., Naider F., Thorner J. and Stevens R. C. (1997) Expression and purification of the Saccharomyces cerevisiae alpha- factor receptor (Ste2p) a 7-transmembrane-segment G protein-coupled receptor. J. Biol. Chem. 272: 15553-15561
61. Andersen B. and Stevens R. C. (1998) The human D1A dopamine receptor: heterologous expression in Saccharomyces cerevisiae and purification of the functional receptor. Protein Expr. Purif. 13: 111-119
62. Kobilka B. K. (1995) Amino and carboxyl terminal modifications to facilitate the production and purification of a G protein-coupled receptor. Anal. Biochem. 231: 269-271
63. Hayashi M. K. and Haga T. (1996) Purification and functional reconstitution with GTP-binding regulatory proteins of hexahistidine-tagged muscarinic acetylcholine receptors (m2 subtype). J. Biochem. (Tokyo) 120: 1232-1238
64. Hampe W., Voss R. H., Haase W., Boege F., Michel H. and Reilander H. (2000) Engineering of a proteolytically stable human beta 2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells. J. Biotechnol. 77: 219-234
65. Ghanouni P., Schambye H., Seifert R., Lee T. W., Rasmussen S. G., Gether U. et al. (2000) The effect of pH on beta(2) adrenoceptor function: evidence for protonation-dependent activation. J. Biol. Chem. 275: 3121-3127
66. Kwatra M. M., Schreurs J., Schwinn D. A., Innis M. A., Caron M. G. and Lefkowitz R. J. (1995) Immunoaffinity purification of epitope-tagged human beta 2-adrenergic receptor to homogeneity. Protein Expr. Purif. 6: 717-721
67. Zeng F. Y. and Wess J. (1999) Identification and molecular characterization of m3 muscarinic receptor dimers. J. Biol. Chem. 274: 19487-19497
68. Vu-Hai M. T., Huet J. C., Echasserieau K., Bidart J. M., Floiras C., Pernollet J. C. et al. (2000) Posttranslational modifications of the lutropin receptor: mass spectrometric analysis. Biochemistry 39: 5509-5517
69. Eroglu C., Cronet P., Panneels V., Beaufils P. and Sinning I. (2002) Functional reconstitution of purified metabotropic glutamate receptor expressed in the fly eye. EMBO Rep. 3: 491-496
70. Panneels V., Eroglu C., Cronet P. and Sinning I. (2003) Pharmacological characterization and immunoaffinity purification of metabotropic glutamate receptor from Drosophila overexpressed in Sf9 cells. Protein Expr. Purif. 30: 275-282
71. Oprian D. D., Molday R. S., Kaufman R. J. and Khorana H. G. (1987) Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc. Natl. Acad. Sci. USA 84: 8874-8878
72. Reeves P. J., Callewaert N., Contreras R. and Khorana H. G. (2002) Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line. Proc. Natl. Acad. Sci. USA 99: 13419-13424
73. Reeves P. J., Kim J. M. and Khorana H. G. (2002) Structure and function in rhodopsin: a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants. Proc. Natl. Acad. Sci. USA 99: 13413-13418
74. Mollaaghababa R., Davidson F. F., Kaiser C. and Khorana H. G. (1996) Structure and function in rhodopsin: expression of functional mammalian opsin in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 93: 11482-11486
75. Shimada M., Chen X., Cvrk T., Hilfiker H., Parfenova M. and Segre G. V. (2002) Purification and characterization of a receptor for human parathyroid hormone and parathyroid hormone-related peptide. J. Biol. Chem. 277: 31774-31780
76. Weng K., Lu C., Daggett L. P., Kuhn R., Flor P. J., Johnson E. C. et al. (1997) Functional coupling of a human retinal metabotropic glutamate receptor (hmGluR6) to bovine rod transducin and rat Go in an in vitro reconstitution system. J. Biol. Chem. 272: 33100-33104
77. Vicentic A., Robeva A., Rogge G., Uberti M. and Minneman K. P. (2002) Biochemistry and pharmacology of epitope-tagged alpha(1)-adrenergic receptor subtypes. J. Pharmacol. Exp. Ther. 302: 58-65
78. Robeva A. S., Woodard R., Luthin D. R., Taylor H. E. and Linden J. (1996) Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope: development of an efficient generic protein purification procedure. Biochem. Pharmacol. 51: 545-555
79. Hopp W. Prickett K. S. Price V. L. Libby R. T. March C. J. Cerretti et al. (1998) A short polypeptide marker sequence useful for recombinant protein identification and purification. Biotechnology 6: 1204-1210
80. Mirzabekov T., Kontos H., Farzan M., Marasco W. and Sodroski J. (2000) Paramagnetic proteoliposomes containing a pure native and oriented seven-transmembrane segment protein CCR5. Nat. Biotechnol. 18: 649-654
81. Mason J. N., Kozell L. B. and Neve K. A. (2002) Regulation of dopamine D(1) receptor trafficking by protein kinase A-dependent phosphorylation. Mol. Pharmacol. 61: 806-816
82. Pang L,. Graziano M. and Wang S. (1999) Membrane cholesterol modulates galanin-GalR2 interaction. Biochemistry 38: 12003-12011
83. Albert A. D., Young J. E. and Yeagle P. L. (1996) Rhodopsin-cholesterol interactions in bovine rod outer segment disk membranes. Biochim. Biophys. Acta 1285: 47-55
84. Gimpl G. and Fahrenholz F. (2002) Cholesterol as stabilizer of the oxytocin receptor. Biochim. Biophys. Acta 1564: 384-392
85. Peterson G. L., Toumadje A., Johnson W. C. Jr and Schimerlik M. I. (1995) Purification of recombinant porcine m2 muscarinic acetylcholine receptor from Chinese hamster ovary cells: circular dichroism spectra and ligand binding properties. J. Biol. Chem. 270: 17808-17814
86. Foord S. M., Bonner T. I., Neubig R. R., Rosser E. M., Pin J. P., Davenport A. P. et al. (2005) International Union of Pharmacology. XLVI. G protein-coupled receptor list. Pharmacol. Rev. 57: 279-288
87. West A. P. Jr, Llamas L. L., Snow P. M., Benzer S. and Bjorkman P. J. (2001) Crystal structure of the ectodomain of Methuselah a Drosophila G protein-coupled receptor associated with extended lifespan. Proc. Natl. Acad. Sci. USA 98: 3744-3749
88. Fan Q. R. and Hendrickson W. A. (2005) Structure of human follicle-stimulating hormone in complex with its receptor. Nature 433: 269-277
89. Grace C. R., Perrin M. H., DiGruccio M. R., Miller C. L., Rivier J. E., Vale W. W. et al. (2004) NMR structure and peptide hormone binding site of the first extracellular domain of a type B1 G protein-coupled receptor. Proc. Natl. Acad. Sci. USA 101: 12836-12841
90. Lundstrom K. (2005) Structural genomics of GPCRs. Trends Biotechnol. 23: 103-108
91. Lundstrom K. (2005) Structural biology of G protein-coupled receptors. Bioorg. Med. Chem. Lett. 15: 3654-3657
92. Warne T., Chirnside J. and Schertler G. F. (2003) Expression and purification of truncated non-glycosylated turkey beta-adrenergic receptors for crystallization. Biochim. Biophys. Acta 1610: 133-140
93. Kwatra M. M., Schwinn D. A., Schreurs J., Blank J. L., Kim C. M., Benovic J. L. et al. (1993) The substance P receptor which couples to Gq/11 is a substrate of beta-adrenergic receptor kinase 1 and 2. J. Biol. Chem. 268: 9161-9164
94. White J. F., Trinh L. B., Shiloach J. and Grisshammer R. (2004) Automated large-scale purification of a G protein-coupled receptor for neurotensin. FEBS. Lett. 564: 289-293
95. Busuttil B. E., Turney K. L. and Frauman A. G. (2001) The expression of soluble full-length recombinant human TSH receptor in a prokaryotic system. Protein Expr. Purif. 23: 369-373
96. Gan L., Alexander J. M., Wittelsberger A., Thomas B. and Rosenblatt M. (in press) Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI(-) cells. Protein Expr. Purif.
97. Asmann Y. W., Dong M. and Miller L. J. (2004) Functional characterization and purification of the secretin receptor expressed in baculovirus-infected insect cells. Regul. Pept. 123: 217-223
98. Alves I. D., Cowell S. M., Salamon Z,. Devanathan S., Tollin G. and Hruby V. J. (2004) Different structural states of the proteolipid membrane are produced by ligand binding to the human delta-opioid receptor as shown by plasmon-waveguide resonance spectroscopy. Mol. Pharmacol. 65: 1248-1257
99. Kim T. K., Zhang R., Feng W., Cai J., Pierce W. and Song Z. H. (2005) Expression and characterization of human CB1 cannabinoid receptor in methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 40: 60-70
100. Feng W, Cai J., Pierce W. M. Jr. and Song Z. H. (2002) Expression of CB2 cannabinoid receptor in Pichia pastoris. Protein Expr. Purif. 26: 496-505
101. Filppula S., Yaddanapudi S., Mercier R., Xu W,. Pavlopoulos S. and Makriyannis A. (2004) Purification and mass spectroscopic analysis of human CB2 cannabinoid receptor expressed in the baculovirus system. J. Pept. Res. 64: 225-236
102. Xu W., Filppula S. A., Mercier R., Yaddanapudi S., Pavlopoulos S., Cai J. et al. (2005) Purification and mass spectroscopic analysis of human CB1 cannabinoid receptor functionally expressed using the baculovirus system. J. Pept. Res. 66: 138-150
103. Yeliseev A. A., Wong K. K., Soubias O. and Gawrisch K. (2005) Expression of human peripheral cannabinoid receptor for structural studies. Protein Sci. 14: 2638-2653
104. Klaassen C. H., Bovee-Geurts P. H., Decaluwe G. L. and DeGrip W. J. (1999) Large-scale production and purification of functional recombinant bovine rhodopsin with the use of the baculovirus expression system. Biochem. J. 342: 293-300
105. Devesa F., Chams V., Dinadayala P., Stella A., Ragas A., Auboiroux H. et al. (2002) Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes. Eur. J. Biochem. 269: 5163-5174
106. Blackburn P. E., Simpson C. V., Nibbs R. J., O'Hara M., Booth R., Poulos J. et al. (2004) Purification and biochemical characterization of the D6 chemokine receptor. Biochem. J. 379: 263-272
107. Shi C., Shin Y. O., Hanson J., Cass B., Loewen M. C. and Durocher Y. (2005) Purification and characterization of a recombinant G-protein-coupled receptor Saccharomyces cerevisiae Ste2p transiently expressed in HEK293 EBNA1 cells. Biochemistry 44: 15705-15714
108. Zhang L., Salom D., He J., Okun A., Ballesteros J., Palczewski K. et al. (2005) Expression of functional G protein-coupled receptors in photoreceptors of transgenic Xenopus laevis. Biochemistry 44: 14509-14518