Expression of CB2 cannabinoid receptor in Pichia pastoris

Protein Expression and Purification

Volumn 26, Issue 3, 2002, Pages 496-505

  Feng, Wenke ^a   Cai, Jian ^a   Pierce Jr , William M ^a   Song, Zhao Hui ^a  

^a University of Louisville School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CANNABINOIDS; GENE EXPRESSION; GLYCOSYLATION; MASS SPECTROMETRY; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PICHIA; RECEPTORS, CANNABINOID; RECEPTORS, DRUG; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

MAMMALIA; PICHIA; PICHIA PASTORIS;

EID: 0036915216     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00569-7     Document Type: Article

References (34)

1. L.A. Matsuda, S.J. Lolait, M.J. Brownstein, A.C. Young, T.I. Bonner, Structure of a cannabinoid receptor and functional expression of the cloned cDNA, Nature 346 (1990) 561-564.
2. S. Munro, K.L. Thomas, M. Abu-Shaar, Molecular characterization of a peripheral receptor for cannabinoids, Nature 365 (1993) 61-65.
3. Y. Gaoni, R. Mechoulam, Isolation, structure and partial synthesis of an active constituent of hashish, J. Am. Chem. Soc. 86(1964) 1646-1647.
4. W.A. Devane, L. Hanus, A. Breuer, R.G. Pertwee, L.A. Stevenson, G. GriRn, D. Gibson, A. Mandelbaum, A. Etinger, R. Mechoulam, Isolation and structure of a brain constituent that binds to the cannabinoid receptor, Science 258 (1992) 1946-1949.
5. A.C. Howlett, Pharmacology of cannabinoid receptors, Annu. Rev. Pharmacol. Toxicol. 35 (1995) 607-634.
6. R.G. Pertwee, Pharmacology of cannabinoid CB1 and CB2 receptors, Pharmacol. Ther. 74 (1997) 129-180.
7. K. Tsou, S. Brown, M.C. Sanudo-Pena, K. Mackie, J.M. Walker, Immunohistochemical distribution of cannabinoid CB1 receptors in the rat central nervous system, Neuroscience 83 (1998) 393-411.
8. S. Galiegue, S. Mary, J. Marchand, D. Dussossoy, D. Carriere, P. Carayon, M. Bouaboula, D. Shire, G. Le Fur, P. Casellas, Expression of central and peripheral cannabinoid receptors in human immune tissues and leukocyte subpopulations, Eur. J. Biochem. 232 (1995) 54-61.
9. N.E. Buckley, S. Hansson, G. Harta, E. Mezey, Expression of the CB1 and CB2 receptor messenger RNAs during embryonic development in the rat, Neuroscience 82 (1998) 1131-1149.
10. W. Feng, Z.H. Song, Functional roles of the tyrosine within the NP(X)(n)Y motif and the cysteines in the C-terminal juxtamembrane region of the CB2 cannabinoid receptor, FEBS Lett. 501 (2001) 166-170.
11. Z.H. Song, C.A. Slowey, D.P. Hurst, P.H. Reggio, The difference between the CB(1) and CB(2) cannabinoid receptors at position 5.46 is crucial for the selectivity of WIN55212-2 for CB(2), Mol. Pharmacol. 56 (1999) 834-840.
12. K.W. Nowell, D.A. Pettit, W.A. Cabral, H.W. Zimmerman Jr., M.E. Abood, G.A. Cabral, High-level expression of the human CB2 cannabinoid receptor using a baculovirus system, Biochem. Pharmacol. 55 (1998) 1893-1905.
13. B. Calandra, J. Tucker, D. Shire, R. Grisshammer, Expression in Escherichia coli and characterization of the human central CB1 and peripheral CB2 cannabinoid receptors, Biotechnol. Lett. 19 (1997) 425-428.
14. M. Schuster, A. Einhauer, E. Wasserbauer, F. Sussenbacher, C. Ortner, M. Paumann, G. Werner, A. Jungbauer, Protein expression in yeast; comparison of two expression strategies regarding protein maturation, J. Biotechnol. 84 (2000) 237-248.
15. M. Schuster, E. Wasserbauer, G. Aversa, A. Jungbauer, Transmembrane-sequence-dependent overexpression and secretion of glycoproteins in Saccharomyces cerevisiae, Protein Expr. Purif. 21 (2001) 1-7.
16. G. Gellissen, Heterologous protein production in methylotrophic yeasts, Appl. Microbiol. Biotechnol. 54 (2000) 741-750.
17. J.L. Cereghino, J.M. Cregg, Heterologous protein expression in the methylotrophic yeast Pichia pastoris, FEMS Microbiol. Rev. 24(2000) 45-66.
18. H.M. Weiss, W. Haase, H. Michel, H. Reilander, Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: Pharmacological characterization and localization, FEBS Lett. 377 (1995) 451-456.
19. F. Talmont, S. Sidobre, P. Demange, A. Milon, L.J. Emorine, Expression and pharmacological characterization of the human mu-opioid receptor in the methylotrophic yeast Pichia pastoris, FEBS Lett. 394 (1996) 268-272.
20. G.M. Cid, P.G. Nugent, A.P. Davenport, R.E. Kuc, B.A. Wallace, Expression and characterization of the human endothelin-A-receptor in Pichia pastoris: Influence of N-terminal epitope tags, J. Cardiovasc. Pharmacol. 36 (2000) S55-S57.
21. C.C.; Felder, J.S.; Veluz, H.L.; Williams, E.M.; Briley, L.A. Matsuda, Cannabinoid agonists stimulate both receptor- and non-receptor-mediated signal transduction pathways in cells transfected with and expressing cannabinoid receptor clones, Mol. Pharmacol. 42 (1992) 838-845.
22. Z.H. Song, C.A. Slowey, D.P. Hurst, P.H. Reggio, The difference between the CB(1) and CB(2) cannabinoid receptors at position 5.46 is crucial for the selectivity of WIN55212-2 for CB(2), Mol. Pharmacol. 56 (1999) 834-840.
23. A. DeBlasi, K. O'Reilly, H.J. Motulsky, Calculating receptor number from binding experiments using same compound as radioligand and competitor, Trends Pharmacol. Sci. 10 (1989) 227-229.
24. Y. Cheng, W.H. Prusoff, Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50% inhibition (I50) of an enzymatic reaction, Biochem. Pharmacol. 22 (1973) 3099-3108.
25. O.N. Jensen, M. Wilm, A. Shevchenko, M. Mann, Sample preparation methods for mass spectrometric peptide mapping directly from 2-DE gels, Methods Mol. Biol. 112 (1999) 513-530.
26. R.S. Fuller, R.E. Sterne, J. Thorner, Enzymes required for yeast prohormone processing, Annu. Rev. Physiol. 50 (1988) 345-362.
27. T. Achstetter, D.H. Wolf, Hormone processing and membrane-bound proteinases in yeast, EMBO J. 4 (1985) 173-177.
28. N.C. Rockwell, G.T. Wang, G.A. Krafft, R.S. Fuller, Internally consistent libraries of fluorogenic substrates demonstrate that Kex2 protease specificity is generated by multiple mechanisms, Biochemistry 36 (1997) 1912-1917.
29. C.D. Strader, T.M. Fong, M.P. Graziano, M.R. Tota, The family of G-protein-coupled receptors, FASEB J. 9 (1995) 745-754.
30. J.M. Baldwin, Structure and function of receptors coupled to G proteins, Curr. Opin. Cell Biol. 6 (1994) 180-190.
31. P.H. Reggio, Ligand-ligand and ligand-receptor approaches to modeling the cannabinoid CB1 and CB2 receptors: Achievements and challenges, Curr. Med. Chem. 6 (1999) 665-683.
32. B. Lagane, G. Gaibelet, E. Meilhoc, J.M. Masson, L. Cezanne, A. Lopez, Role of sterols in modulating the human mu-opioid receptor function in Saccharomyces cerevisiae, J. Biol. Chem. 275 (2000) 33197-33200.
33. B.K. Kobilka, Amino and carboxyl terminal modifications to facilitate the production and purification of a G protein-coupled receptor, Anal. Biochem. 231 (1995) 269-271.
34. A.S. Robeva, R. Woodard, D.R. Luthin, H.E. Taylor, J. Linden, Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure, Biochem. Pharmacol. 51 (1996) 545-555.