Solubilization, purification, and mass spectrometry analysis of the human mu-opioid receptor expressed in Pichia pastoris

Protein Expression and Purification

Volumn 43, Issue 2, 2005, Pages 85-93

  Sarramegna, Valérie ^a,b   Muller, Isabelle ^a   Mousseau, Guillaume ^a   Froment, Carine ^a   Monsarrat, Bernard ^a   Milon, Alain ^a   Talmont, Franck ^a  

^a INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^b UNIVERSITÉ DE LA NOUVELLE CALÉDONIE   (New Caledonia)

Author keywords

Detergent; GFP; GPCR; Mu opioid receptor; Pichia pastoris; Purification; Solubilization

Indexed keywords

PICHIA PASTORIS;

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MU OPIATE RECEPTOR;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; PICHIA;

CHROMATOGRAPHY, AFFINITY; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; HUMANS; PICHIA; RECEPTORS, OPIOID, MU; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 32944474906     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.05.007     Document Type: Article

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