Double tagging recombinant A1-and A2a-adenosine receptors with hexahistidine and the FLAG epitope: Development of an efficient generic protein purification procedure

Biochemical Pharmacology

Volumn 51, Issue 4, 1996, Pages 545-555

  Robeva, Anna S ^a   Woodard, Robin ^a   Luthin, David R ^a   Taylor, Heidi E ^a   Linden, Joel ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

Adenosine receptors; baculovirus; Affinity chromatography; FLAG epitope; Hexahistidine; Protein purification; Recombinant proteins

Indexed keywords

2 [4 (2 CARBOXYETHYL)PHENETHYLAMINO]ADENOSINE 5' (N ETHYLCARBOXAMIDE); 6 N (4 AMINOBENZYL)ADENOSINE; 6 N CYCLOPENTYLADENOSINE; 8 (4 SULFOPHENYL)THEOPHYLLINE; 8 [4 (2 AMINOETHYLCARBAMOYL)METHOXYPHENYL] 1,3 DIPROPYLXANTHINE; 8 CYCLOPENTYL 1,3 DIPROPYLXANTHINE; ADENOSINE 5' (N ETHYLCARBOXAMIDE); ADENOSINE A1 RECEPTOR; ADENOSINE A2A RECEPTOR; ADENOSINE RECEPTOR AFFECTING AGENT; BW A522; COMPLEMENTARY DNA; CYCLIC AMP; EPITOPE; FLAG EPITOPE; FORSKOLIN; HEXAHISTIDINE; HISTIDINE; IODINE 125; RADIOLIGAND; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; BACULOVIRUS; BINDING AFFINITY; BINDING COMPETITION; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; LIGAND BINDING; MAMMAL CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY;

ADENOSINE; ADENOSINE-5'-(N-ETHYLCARBOXAMIDE); ANIMALS; BASE SEQUENCE; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; DNA, COMPLEMENTARY; EPITOPES; HISTIDINE; HUMANS; IODINE RADIOISOTOPES; KINETICS; MAMMALS; MOLECULAR SEQUENCE DATA; OLIGODEOXYRIBONUCLEOTIDES; PEPTIDE BIOSYNTHESIS; PEPTIDES; PHENETHYLAMINES; PLASMIDS; RADIOLIGAND ASSAY; RECEPTORS, PURINERGIC P1; RECOMBINANT PROTEINS; SEQUENCE TAGGED SITES; SPODOPTERA; TRANSFECTION; TRITIUM;

EID: 0030070457     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/0006-2952(95)02235-X     Document Type: Article

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