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Volumn 7, Issue 6, 2001, Pages 453-469

Solubilization and purification of the human ETB endothelin receptor produced by high-level fermentation in Pichia pastoris

(5) Schiller, H a Molsberger, E a Janssen, P a Michel, H a Reilander H a

a SANOFI AVENTIS DEUTSCHLAND GMBH (Germany)

Author keywords

G protein coupled receptor; Human ETB endothelin receptor; Large scale production; Membrane protein; Pichia pastoris; Purification; Solubilization

Indexed keywords

DETERGENT; ENDOTHELIN RECEPTOR; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; LIGAND; METHANOL; RECOMBINANT RECEPTOR;

ARTICLE; BACULOVIRUS; BIOREACTOR; CELL CLONING; CELL DENSITY; COMPARATIVE STUDY; CONTROLLED STUDY; FERMENTATION TECHNIQUE; FUNGAL CELL CULTURE; HETEROLOGOUS EXPRESSION; INSECT CELL; ISOLATION PROCEDURE; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN STABILITY; RECEPTOR SOLUBILIZATION; YEAST CELL;

BINDING SITES; CELL FRACTIONATION; CELL MEMBRANE; CHROMATOGRAPHY, AFFINITY; DETERGENTS; FERMENTATION; GENE EXPRESSION; GENETIC ENGINEERING; HUMANS; MALTOSE; PICHIA; PLASMIDS; RECEPTOR, ENDOTHELIN B; RECEPTORS, ENDOTHELIN; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TRANSFORMATION, GENETIC;

INSECTA; PICHIA; PICHIA PASTORIS; UNIDENTIFIED BACULOVIRUS;

EID: 0035671430 PISSN: 10606823 EISSN: None Source Type: Journal
DOI: None Document Type: Article

Times cited : (19)

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