Engineering of a proteolytically stable human β2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells

Journal of Biotechnology

Volumn 77, Issue 2-3, 2000, Pages 219-234

  Hampe, Wolfgang ^a   Voss, Ralf Holger ^a   Haase, Winfried ^a   Boege, Fritz ^b   Michel, Hartmut ^a   Reiländer, Helmut ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Fusion protein; Human 2 adrenergic receptor; Maltose binding protein; Membrane protein purification; Proteolytic stability

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; CYANOPINDOLOL I 125; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL CULTURE; ESCHERICHIA COLI; GEL ELECTROPHORESIS; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; IMMUNOBLOTTING; IN VITRO STUDY; INSECT; INTERMETHOD COMPARISON; ISOTOPE LABELING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECEPTOR BINDING; TECHNIQUE;

ADRENERGIC BETA-ANTAGONISTS; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BACULOVIRIDAE; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; DIAZOMETHANE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENGINEERING; GTP-BINDING PROTEINS; HUMANS; MONOSACCHARIDE TRANSPORT PROTEINS; PERIPLASMIC BINDING PROTEINS; PHOTOAFFINITY LABELS; PINDOLOL; RECEPTORS, ADRENERGIC, BETA; RECOMBINANT FUSION PROTEINS; SPODOPTERA; TRANSFECTION;

EID: 0033979165     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00216-3     Document Type: Article

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