Optimizing functional versus total expression of the human μ-opioid receptor in Pichia pastoris

Protein Expression and Purification

Volumn 24, Issue 2, 2002, Pages 212-220

  Sarramegna, Valérie ^a   Demange, Pascal ^a   Milon, Alain ^a   Talmont, Franck ^a  

^a INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

Author keywords

EGFP; Expression; Fusion protein; GPCR; Pichia pastoris; opioid receptor

Indexed keywords

PICHIA; PICHIA PASTORIS;

EPIDERMAL GROWTH FACTOR RECEPTOR; HYBRID PROTEIN; METHANOL; MU OPIATE RECEPTOR;

ARTICLE; BIOSYNTHESIS; GENE DOSAGE; GENETICS; HUMAN; METHODOLOGY; MOLECULAR CLONING; PH; PICHIA; PROMOTER REGION; TEMPERATURE;

CLONING, MOLECULAR; GENE DOSAGE; HUMANS; HYDROGEN-ION CONCENTRATION; METHANOL; PICHIA; PROMOTER REGIONS (GENETICS); RECEPTOR, EPIDERMAL GROWTH FACTOR; RECEPTORS, OPIOID, MU; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

EID: 0036514182     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1564     Document Type: Article

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