The human D(1A) dopamine receptor: Heterologous expression in Saccharomyces cerevisiae and purification of the functional receptor

Protein Expression and Purification

Volumn 13, Issue 1, 1998, Pages 111-119

  Andersen, Birgitte ^a,b   Stevens, Raymond C ^c  

^a LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; DOPAMINE; EXPRESSION VECTOR; IMMUNOAFFINITY CHROMATOGRAPHY; PROTEIN PURIFICATION;

SACCHAROMYCES CEREVISIAE;

DOPAMINE 1 RECEPTOR; DOPAMINE D1A RECEPTOR; FLAG PEPTIDE; HYBRID PROTEIN; MATING HORMONE ALPHA FACTOR RECEPTOR; PEPTIDE; PHOSPHOLIPID; PRIMER DNA; RECEPTOR; RECOMBINANT DNA; TRANSCRIPTION FACTOR;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BINDING COMPETITION; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEOTIDE SEQUENCE; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING, COMPETITIVE; CHROMATOGRAPHY, AFFINITY; DNA PRIMERS; DNA, RECOMBINANT; HUMANS; PEPTIDES; PHOSPHOLIPIDS; RECEPTORS, DOPAMINE D1; RECEPTORS, MATING FACTOR; RECEPTORS, PEPTIDE; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION FACTORS;

EID: 0032081297     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0862     Document Type: Article

References (35)

1. Strader C. D., Fong T. M., Graziano M. P., Tota M. R. The family of G-protein-coupled receptors. FASEB J. 9:1995;745-754.
2. Sibley D. R., Monsma F. J., Shen Y. Molecular neurobiology of dopaminergic receptors. Int. Rev. Neurobiol. 35:1993;391-415.
3. Gingrich J. A., Caron M. G. Recent advances in the molecular biology of dopamine receptors. Annu. Rev. Neurosci. 16:1993;299-321.
4. Civelli O., Bunzow J. R., Grandy D. K., Zhou Q.-Y., Van Tol H. H. M. Molecular biology of the dopamine receptors. Eur. J. Pharmacol. 207:1991;277-286.
5. Fraser C. M., Lee N. H., Pellegrino S. M., Kerlevage A. R. Molecular properties and regulation of G-protein-coupled receptors. Prog. Nucleic Acid Res. Mol. Biol. 49:1994;113-156.
6. Livingstone C. D., Strange P. G., Naylor L. H. Molecular modelling of D2-like dopamine receptors. Biochem. J. 287:1992;277-282.
7. Trumpp-Kallmeyer S., Hoflack J., Bruinvels A., Hibert M. Modeling of G-protein-coupled receptors: Application to dopamine, adrenaline, serotonin, acetylcholine, and mammalian opsin receptors. J. Med. Chem. 35:1992;3448-3462.
8. Hibert M. F., Trumpp-Kallmeyer S., Bruinvels A., Hoflack J. Three-dimensional models of neurotransmitter G-binding protein-coupled receptors. Mol. Pharmacol. 40:1991;8-15.
9. Baldwin J. M. Structure and function of receptors coupled to G proteins. Curr. Opin. Cell Biol. 6:1994;180-190.
10. Nekrasova E., Sosinskaya A., Natochin M., Lancet D., Gat U. Overexpression, solubilization and purification of rat and human olfactory receptors. Eur. J. Biochem. 238:1996;28-37.
11. King K., Dohlman H. G., Thorner J., Caron M. G., Lefkowitz R. J. Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. Science. 250:1990;121-123.
12. Huang H. J., Liao C. F., Yang B. C., Kuo T. T. Functional expression of rat M5 muscarinic acetylcholine receptor in yeast. Biochem. Biophys. Res. Commun. 182:1992;1180-1186.
13. Payette P., Gossard F., Whiteway M., Dennis M. Expression and pharmacological characterization of the human M1 muscarinic receptor in Saccharomyces cerevisiae. FEBS Lett. 266:1990;21-25.
14. Sander P., Grünewald S., Bach M., Haase W., Reiländer H., Michel H. Heterologous expression of the human D2S dopamine receptor in protease-deficient Saccharomyces cerevisiae strains. Eur. J. Biochem. 226:1994;697-705.
15. Sander P., Grünewald S., Reiländer H., Michel H. Constitutive expression of the human D2S-dopamine receptor in the unicellular yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta. 1193:1994;255-262.
16. Sander P., Grünewald S., Reiländer H., Michel H. Expression of the human D2S dopamine receptor in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe: A comparative study. FEBS Lett. 344:1994;41-46.
17. Grandy D. K., Zhou Q. Y., Allen L., Litt R., Magenis R. E., Civelli O., Litt M. A human D1 dopamine receptor gene is located on chromosome 5 at q35.1 and identifies an EcoRI RFLP. Am. J. Human Gen. 47:1990;828-834.
18. Zhou Q. Y., Grandy D. K., Thambi L., Kushner J. A., Van Tol H. H., Cone R., Pribnow D., Salon J., Bunzow J. R., Civelli O. Cloning and expression of human and rat D1 dopamine receptors. Nature. 347:1990;76-80.
19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
20. Schena M., Picard D., Yamamoto K. R. Vectors for constitutive and inducible gene expression in yeast. Methods Enzymol. 194:1991;389-398.
21. David N. E., Gee M., Andersen B., Naider F., Thorner J., Stevens R. Expression and purification of the Saccharomyces cerevisiae alpha-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor. J. Biol. Chem. 272:1997;15553-15561.
22. Sherman F., Fink G. R., Lawrence C. W. Methods in Yeast Genetics. 1979;Cold Spring Harbor Laboratory, Cold Spring Harbor.
23. Venema K., Palmgren M. G. Metabolic modulation of transport coupling ratio in yeast plasma membrane H(+)-ATPase. J. Biol. Chem. 270:1995;19659-19667.
24. Villalba J. M., Palmgren M. G., Berberian G. E., Ferguson C., Serrano R. Functional expression of plant plasma membrane H(+)-ATPase in yeast endoplasmic reticulum. J. Biol. Chem. 267:1992;12341-12349.
25. Sidhu A. Solubilization and reconstitution of the D-1 dopamine receptor: Potentiation of the agonist high-affinity state of the receptor. Biochemistry. 33:1988;8786-8776.
26. Fling S. P., Gregerson D. S. Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Anal. Biochem. 155:1986;83-88.
27. Sidhu A., Kimura K., Vachvanichsanong P. Induction of G protein-independent agonist high-affinity binding sites of D-1 dopamine receptors by beta-mercaptoethanol. Biochemistry. 33:1994;11246-11253.
28. Laruelle M., Sidhu A., Casanova M. F., Weinberger D. R., Kleinman J. E. Characterization of [125I]SCH 23982 binding in human brain: Comparison with [3H]SCH 23390. Neurosci. Lett. 131:1991;273-276.
29. Balen P., Kimura K., Sidhu A. Specific phospholipid requirements for the solubilization and reconstitution of D-1 dopamine receptors from striatal membranes. Biochemistry. 33:1994;1539-1544.
30. Niznik H. B., Otsuka N. Y., Dumbrille-Ross A., Grigoriadis D., Tirpak A., Seeman P. Dopamine D1 receptors characterized with [3H]SCH 23390. Solubilization of a guanine nucleotide-sensitive form of the receptor. J. Biol. Chem. 261:1986;8397-8406.
31. Kimura K., Sidhu A. Temperature sensitivity of agonist high-affinity binding sites of solubilized and reconstituted D1 dopamine receptors. J. Neurochem. 63:1994;2093-2098.
32. Sidhu A., Kimura K. Ascorbic acid inhibits 125I-SCH 23982 binding but increases the affinity of dopamine for D1 dopamine receptors. J. Neurochem. 63:1994;201-206.
33. Potter L. T., Ballesteros L. A., Bichajian L. H., Ferrendelli C. A., Fisher A., Hanchett H. E., Zhang R. Evidence of paired M2 muscarinic receptors. Mol. Pharmacol. 39:1991;211-221.
34. Ng G. Y., O'Dowd B. F., Lee S. P., Chung H. T., Brann M. R., Seeman P., George S. R. Dopamine D2 receptor dimers and receptor-blocking peptides. Biochem. Biophys. Res. Commun. 227:1996;200-204.
35. Hebert T. E., Moffett S., Morello J. P., Loisel T. P., Bichet D. G., Barret C., Bouvier M. A peptide derived from a beta2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation. J. Biol. Chem. 271:1996;16384-16392.