Purification and Functional Reconstitution of the Human P2Y12 Receptor

Molecular Pharmacology

Volumn 64, Issue 5, 2003, Pages 1210-1216

  Bodor, Erik T ^a   Waldo, Gary L ^a   Hooks, Shelley B ^a   Corbitt, James ^a   Boyer, Jose L ^a   Harden, T Kendall ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 METHYLTHIOADENOSINE DIPHOSPHATE; 2 METHYLTHIOADENOSINE TRIPHOSPHATE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; BACULOVIRUS VECTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; NUCLEOTIDE; PROTEIN; PURINE P2Y RECEPTOR; PURINE P2Y12 RECEPTOR; RECOMBINANT PURINE P2Y12 RECEPTOR; RGS PROTEIN; RGS4 PROTEIN; UNCLASSIFIED DRUG;

ALPHA CHAIN; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; INSECT CELL; LIPID VESICLE; NONHUMAN; NUCLEOTIDE METABOLISM; PHOSPHOLIPID VESICLE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEOLIPOSOME; SIGNAL TRANSDUCTION; THROMBOCYTE;

ADENOSINE DIPHOSPHATE; ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; HUMANS; MEMBRANE PROTEINS; RECEPTORS, PURINERGIC P2; TRANSFECTION;

EID: 0142148089     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.64.5.1210     Document Type: Article

References (39)

1. Abbracchio MP, Boeynaems J-M, Barnard EA, Boyer JL, Kennedy C, Miras-Portugal MT, King BF, Gachet C, Jacobson KA, Weisman GA, et al. (2003) Characterization of the UDP-glucose receptor (re-named here the P2Y14 receptor) adds diversity to the P2Y receptor family. Trends Pharmacol Sci 24:52-55.
2. 2Y-purinergic receptor that inhibits adenylyl cyclase but does not activate phospholipase C. J Pharmacol Exp Ther 267:1140-1146.
3. 1 receptor: pharmacological, signaling and molecular properties. Drug Dev Res 39:253-261.
4. Burnstock G (1972) Purinergic nerves. Pharmacol Rev 24:509-581.
5. i. J Biol Chem 276:41479-41485.
6. Cooper DMF and Rodbell M (1979) ADP is a potent inhibitor of human platelet plasma membrane adenylate cyclase. Nature (Lond) 282:517-518.
7. Dangelmaier C, Jin J, Daniel JL, Smith JB, and Kunapuli SP (2000) The P2Y1 receptor mediates ADP-induced p38 kinase-activating factor generation in human platelets. Eur J Biochem 267:2283-2289.
8. Daniel JL, Dangelmaier C, Jin J, Ashby B, Smith B, and Kunapuli SP (1998) Molecular basis for ADP-induced platelet activation. I. Evidence for three distinct ADP receptors on human platelets. J Biol Chem 273:2024-2029.
9. Fabre JE, Nguyen M, Latour A, Keifer JA, Audoly LP, Coffman TM, and Koller BH (1999) Decreased platelet aggregation, increased bleeding time and resistance to thromboembolism in P2Y1-deficient mice. Nat Med 5:1199-1202.
10. 2Y-purinergic receptor that couples to phospholipase C. Mol Pharmacol 46:8-14.
11. Foster CJ, Prosser DM, Agans JM, Zhai Y, Smith MD, Lachowicz JE, Zhang FL, Gustafson E, Monsma FJ Jr, Wiekowski MT, et al. (2001) Molecular identification and characterization of the platelet ADP receptor targeted by thienopyridine antithrombotic drugs. J Clin Investig 107:1591-1598.
12. Fredholm BB, Abbracchio MP, Burnstock G, Dubyak GR, Harden TK, Jacobson KA, Schwabe U, and Williams M (1997) Towards a revised nomenclature for P1 and P2 receptors. Trends Pharmacol Sci 18:79-82.
13. Gagnon AW, Manning DR, Catani L, Gewirtz A, Poncz M, and Brass LF (1991) Identification of Gz alpha as a pertussis toxin-insensitive G protein in human platelets and megakaryocytes. Blood 78:1247-1253.
14. Harden TK, Barnard EA, Boeynaems JM, Burnstock G, Dubyak GR, Hourani SMO, and Insel PA (1998) P2Y receptors, in The IUPHAR Compendium of Receptor Characterization and Classification (Girdlestone D ed) pp 209-217, IUPHAR Media, London.
15. 2-purinergic receptors: subtype-associated signaling responses and structure. Annu Rev Pharmacol Toxicol 35:541-579.
16. Harden TK, Lazarowski ER, and Boucher RC (1997) Release, metabolism and interconversion of adenine and uridine nucleotides: implications for G protein-coupled P2 receptor agonist selectivity. Trends Pharmacol Sci 18:43-46.
17. Hechler B, Eckly A, Ohlmann P, Cazenave JP, and Gachet C (1998a) The P2Y1 receptor, necessary but not sufficient to support full ADP-induced platelet aggregation, is not the target of the drug clopidogrel. Br J Haematol 103:858-866.
18. Hechler B, Leon C, Vial C, Vigne P, Frelin C, Cazenave JP, and Gachet C (1998b) The P2Y1 receptor is necessary for adenosine 5′ -diphosphate-induced platelet aggregation. Blood 92:152-159.
19. Hollopeter G, Jantzen HM, Vincent D, Li G, England L, Ramakrishnan V, Yang RB, Nurden P, Nurden A, Julius D, et al. (2001) Identification of the platelet ADP receptor targeted by antithrombotic drugs. Nature (Lond) 409:202-207.
20. Jantzen HM, Milstone DS, Gousset L, Conley PB, and Mortensen RM (2001) Impaired activation of murine platelets lacking G alpha(i2). J Clin Investig 108:477-483.
21. Jin J and Kunapuli SP (1998) Coactivation of two different G protein-coupled receptors is essential for ADP-induced platelet aggregation. Proc Natl Acad Sci USA 95:8070-8074.
22. Kozasa T and Gilman AG (1995) Purification of recombinant G proteins from Sf9 cells by hexahistidine tagging of associated subunits. J Biol Chem 270:1734-1741.
23. Lazarowski ER and Boucher RC (2001) UTP as an extracellular signaling molecule. News Physiol Sci 16:1-5.
24. Leon C, Hechler B, Freund M, Eckly A, Vial C, Ohlmann P, Dierich A, LeMeur M, Cazenave JP, and Gachet C (1999) Defective platelet aggregation and increased resistance to thrombosis in purinergic P2Y(1) receptor-null mice. J Clin Investig 104:1731-1737.
25. Offermanns S, Toombs CF, Hu YH, and Simon MI (1997) Defective platelet activation in G alpha(q)-deficient mice. Nature (Lond) 389:183-186.
26. Ohlmann P, Laugwitz KL, Nurnberg B, Spicher K, Schultz G, Cazenave JP, and Gachet C (1995) The human platelet ADP receptor activates Gi2 proteins. Biochem J 312:775-779.
27. 1 receptor. Mol Pharmacol 54:1118-1123.
28. Park HS and Hourani SM (1999) Differential effects of adenine nucleotide analogues on shape change and aggregation induced by adenosine 5-diphosphate (ADP) in human platelets. Br J Pharmacol 127:1359-1366.
29. Parker EM, Kameyama K, Higashijima T, and Ross EM (1991) Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells. J Biol Chem 266:519-527.
30. Ralevic V and Burnstock G (1998) Receptors for purines and pyrimidines. Pharmacol Rev 50:413-492.
31. Resendiz JC, Feng S, Ji G, Francis KA, Berndt MC, and Kroll MH (2003) Purinergic P2Y12 receptor blockade inhibits shear-induced platelet phosphatidylinositol 3-kinase activation. Mol Pharmacol 63:639-645.
32. Saugstad JA, Marino MJ, Folk JA, Hepler JR, and Conn PJ (1998) RGS4 inhibits signaling by group I metabotropic glutamate receptors. J Neurosci 18:905-913.
33. Savi P, Beauverger P, Labouret C, Delfaud M, Salel V, Kaghad M, and Herbert JM (1998) Role of P2Y1 purinoceptor in ADP-induced platelet activation. FEBS Lett 422:291-295.
34. 12 receptor. Br J Pharmacol 118:167-173.
35. 12 nucleotide receptor of brain capillary endothelial cells. J Biol Chem 277:31390-31400.
36. 1 receptor. Mol Pharmacol 60:432-439.
37. Webb TE, Simon J, Krishek BJ, Bateson AN, Smart TG, King BF, Burnstock G, and Barnard EA (1993) Cloning and functional expression of a brain G-protein-coupled ATP receptor. FEBS Lett 324:219-225.
38. Williams AG, Woolkalis MJ, Poncz M, Manning DR, Gewirtz AM, and Brass LF (1990) Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryoctes and human erythroleukemia cells. Blood 76:721-730.
39. Zhang FL, Luo L, Gustafson E, Lachowicz J, Smith M, Qiao X, Liu YH, Chen G, Pramanik B, Laz TM, et al. (2001) ADP is the cognate ligand for the orphan G-protein coupled receptor SP1999. J Biol Chem 276:8608-8615.