Antibody arrays for high-throughput screening of antibody-antigen interactions

Nature Biotechnology

Volumn 18, Issue 9, 2000, Pages 989-994

  De Wildt, Ruud M T ^a   Mundy, Chris R ^b   Gorick, Barbara D ^a   Tomlinson, Ian M ^a  

^a MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

^b United Kingdom Human Genome Mapping Project Resource Centre   (United Kingdom)

Author keywords

Antibody; Array screening; High throughput; Phage display; Single chain variable fragment (scFv)

Indexed keywords

RECOMBINANT ANTIBODY;

ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; MOLECULAR CLONING; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; TECHNIQUE;

AMINO ACID SEQUENCE; ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; BACTERIA; BIOCHEMISTRY; BIOSENSING TECHNIQUES; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; HELA CELLS; HUMANS; MOLECULAR PROBE TECHNIQUES; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PEPTIDE LIBRARY; PROTEIN CONFORMATION; PROTEINS; RECOMBINANT PROTEINS; ROBOTICS; SERUM ALBUMIN; SERUM ALBUMIN, BOVINE;

EID: 0034425742     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/79494     Document Type: Article

References (41)

1. Making antibodies by phage display technology
2. Isolation of high affinity human antibodies directly from large synthetic repertoires
3. Characterization of human variable domain antibody fragments against the U1 RNA-associated A protein, selected from a synthetic and a patient-derived combinatorial V gene library
4. Human anti-self antibodies with high specificity from phage display libraries
5. Efficient construction of a large nonimmune phage antibody library: The production of high-affinity human single-chain antibodies to protein antigens
6. A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies
7. Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage antibody library
8. Directing phage selections towards specific epitopes
9. Selection-dominant and nonaccessible epitopes on cell-surface receptors revealed by cell-panning with a large phage antibody library
10. Limited diversity of human scFv fragments isolated by panning a synthetic phage-display scFv library with cultured human melanoma cells
11. Efficient isolation of genes by using antibody probes
12. Identification of human antibody fragment clones specific for tetanus toxoid in a bacteriophage lambda immunoexpression library
13. Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
14. Discovery of human antibodies to cell surface antigens by capture lift screening of phage-expressed antibody libraries
15. Filter screening of antibody Fab fragments secreted from individual bacterial colonies: Specific detection of antigen binding with a two-membrane system
16. Production of a single chain anti-CEA antibody from the hybridoma cell line T84.66 using a modified colony-lift selection procedure to detect antigen-positive scFv bacterial clones
17. Making antibody fragments using phage display libraries
18. Human anti-nicotinic acetylcholine receptor recombinant Fab fragments isolated from thymus-derived phage display libraries from myasthenia gravis patients reflect predominant specificities in serum and block the action of pathogenic serum antibodies
19. Anti-melanoma antibodies from melanoma patients immunized with genetically modified autologous tumor cells: Selection of specific antibodies from single-chain Fv fusion phage libraries
20. Human monoclonal antibody fragments from combinatorial antibody libraries directed to the U1 snRNP associated U1C protein; epitope mapping, immunolocalization and V-gene usage
21. Selection of phage antibodies by binding affinity, mimicking affinity maturation
22. By-passing immunization: Building high affinity human antibodies by chain shuffling
23. Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection
24. Proteomics: Quantitative and physical mapping of cellular proteins
25. Exploring the new world of the genome with DNA microarrays
26. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library
27. x from a phage display library
28. x
29. Diabodies: Small bivalent and bispecific antibody fragments
30. Triabodies: Single chain Fv fragments without a linker form trivalent trimers
31. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays
32. Pathfinder selection: In situ isolation of novel antibodies
33. A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library
34. Protein microarrays for gene expression and antibody screening
35. Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies
36. Screening for autoantibodies to the nucleotar U3- and Th(7-2) ribonucleoproteins in patients sera using antisense riboprobes
37. Analysis of heavy and light chain pairings indicates that receptor editing shapes the human antibody repertoire
38. Proteolytic selection for protein folding using filamentous bacteriophages
39. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay
40. Competition BIAcore for measuring true affinities: Large differences from values determined from binding kinetics