Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS)

Nature Biotechnology

Volumn 19, Issue 6, 2001, Pages 537-542

  Chen, Gang ^a   Hayhurst, Andrew ^a   Thomas, Jeffery G ^a,b   Harvey, Barrett R ^a   Iverson, Brent L ^a   Georgiou, George ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b EPIGENOMICS AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOPHAGES; CELL MEMBRANES; CLONING; ESCHERICHIA COLI; FLUORESCENCE; PROTEINS;

FLOW CYTOMETRY;

BIOTECHNOLOGY;

DIGOXIGENIN;

ARTICLE; BACTERIAL MEMBRANE; BACTERIOPHAGE; BINDING AFFINITY; CELL ISOLATION; CONTROLLED STUDY; CYTOPLASM; DNA LIBRARY; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL;

BIOCHEMISTRY; DIGOXIGENIN; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLOW CYTOMETRY; IMMUNOGLOBULIN FRAGMENTS; LIGANDS; MUTATION; PEPTIDE LIBRARY; PERIPLASM; PLASMIDS; PROTEIN BINDING; SURFACE PLASMON RESONANCE; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FILAMENTOUS BACTERIOPHAGE; NEGIBACTERIA; UNIDENTIFIED BACTERIOPHAGE;

EID: 0035014528     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/89281     Document Type: Article

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