Disrupting the hydrophobic patches at the antibody variable/constant domain interface: Improved in vivo folding and physical characterization of an engineered scFv fragment

Protein Engineering

Volumn 10, Issue 4, 1997, Pages 435-444

  Nieba, Lars ^a   Honegger, Annemarie ^a   Krebber, Claus ^a   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Antibody engineering; Domain interface; Hydrophobic patches; Protein aggregation; Protein engineering; Single chain Fv fragment

Indexed keywords

ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CYTOPLASM; EXPERIMENTAL MODEL; GENETIC ENGINEERING; IMMUNOGLOBULIN STRUCTURE; IMMUNOGLOBULIN VARIABLE REGION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN FOLDING;

ESCHERICHIA COLI;

EID: 0030965970     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.4.435     Document Type: Article

References (56)

1. Bedzyk, W.D., Weidner, K.M., Denzin, L.K., Johnson, L.S., Hardman, K.D., Pantoliano, M.W., Asel, E.D. and Voss, E.W., Jr (1990) J. Biol. Chem., 265, 18615-18620.
2. Bird, R.E.., Hardman, K.D., Jacobson, J.W., Johnson, S., Kaufman, B.M., Lee, S.M., Lee, T., Pope, S.H., Riordan, G.S. and Whitlow, M. (1988) Science, 242, 423-426.
3. Brinkmann, U., Reiter, Y., Jung, S., Lee, B. and Pastan, I. (1993) Proc. Natl Acad. Sci. USA, 90, 7538-7542.
4. Carter, P. et al. (1992) Bio/Technology, 10, 163-167.
5. Chan, W., Helms, L.R., Brooks, I., Lee, G., Ngola, S., McNulty, D., Maleeff, B., Hensley, P. and Wetzel, R. (1996) Folding Des., 1, 77-89.
6. Chatellier, J., van Regenmortel, M.H.V., Vernet, T. and Altschuh, D. (1996) J. Mol. Biol., 264, 1-6.
7. Chothia, C. et al. (1989) Nature, 342, 877-883.
8. Crameri, A., Whitehorn, E.A., Tate, E. and Stemmer, W.P.C. (1996) Nature Biotechnol., 14, 315-319.
9. Dale, G.E., Broger, C., Langen, H., D'Arcy, A. and Stüber, D. (1994) Protein Engng, 7, 933-939.
10. Davies, J. and Riechmann, L. (1994) FEBS Lett., 339, 285-290.
11. Davies, J. and Riechmann, L. (1996) Protein Engng, 9, 531-537.
12. Denzin, L.K., Whitlow, M. and Voss, E.W., Jr (1991) J. Biol. Chem., 266, 14095-14102.
13. Desmyter, A., Transue, T.R., Ghahroudi, M.A., Thi, M.D., Poortmans, F., Hamers, R., Muyldermans, S. and Wyns, L. (1996) Nature Struct. Biol., 3, 803-811.
14. Desplancq, D., King, D.J., Lawson, A.D. and Mountain, A. (1994) Protein Engng, 7, 1027-1033.
15. Ge, L., Knappik, A., Pack, P., Freund, C. and Plückthun, A. (1995) In Borrebaeck, C.A.K. (ed.), Antibody Engineering. A Practical Approach. IRL Press, Oxford, pp. 229-266.
16. Gill, S.C. and von Hippel, P.H. (1989) Anal. Biochem., 182, 319-326.
17. Glockshuber, R., Malia, M., Pfitzinger, I. and Plückthun, A. (1990) Biochemistry, 29, 1362-1366.
18. Holliger, P., Prospero, T. and Winter, G. (1993) Proc. Natl Acad. Sci. USA, 90, 6444-6448.
19. Horn, U. et al. (1996) Appl. Microbiol. Biotechnol., 46, 524-532.
20. Huston, J.S., Levinson, D., Mudgett-Hunter, M., Tai, M.S., Novotny, J., Margolies, M.N., Ridge, R.J., Bruccoleri, R.E., Haber, E., Crea, R. and Oppermann, H. (1988) Proc. Natl Acad. Sci. USA, 85, 5879-5883.
21. Huston, J., George, A.J.T., Tai, M., McCartney, J.E., Jin, D., Segal, D.M., Keck, P. and Oppermann, H. (1995) In Borrebaeck, C.A.K. (ed.), Antibody Engineering. A Practical Approach. IRL Press, Oxford, pp. 185-228.
22. Kabat, E.A., Wu, T.T., Perry, H.M., Gottesmann, K.S. and Foeller, C. (1991) NIH Publication 91-3242. US Department of Health and Human Services, Public Health Service, National Institutes of Health, Washington, DC.
23. Knappik, A. and Plückthun, A. (1994) BioTechniques, 17, 754-761.
24. Knappik, A. and Plückthun, A. (1995) Protein Engng, 8, 81-89.
25. Kortt, A.A., Guthrie, R.E., Hinds, M.G., Power, B.E., Ivancic, N., Caldwell, J.B., Gruen, L.C., Norton, R.S. and Hudson, P.J. (1995) J. Protein Chem., 14, 167-178.
26. Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) Methods Enzymol., 154, 367-382.
27. Lee, B. and Richards, F.M. (1971) J. Mol. Biol., 55, 379-400.
28. Lesk, A.M. and Chothia, C. (1988) Nature, 335, 188-190.
29. Leistler, B. and Perham, R.N. (1994) Biochemistry, 33, 2773-2781.
30. Lindner, P, Guth, B., Wülfing, C., Krebber, C., Steipe, B., Müller, F. and Plückthun, A. (1992) Methods: Companion Methods Enzymol., 4, 41-56.
31. Middaugh, C.R., Tisel, W.A., Haire, R.N. and Rosenberg, A. (1979) J. Biol. Chem., 254, 367-370.
32. Miklasz, S.D., Gulliver, G.A. and Voss, E.W., Jr (1995) J. Mol. Recogn., 8, 258-269.
33. Novotny, J., Ganju, R.K., Smiley, S.T., Hussey, R.E., Luther, M.A., Recny, M.A., Siliciano, R.F. and Reinherz, E.L. (1991) Proc. Natl Acad. Sci. USA, 88, 8646-8650.
34. Pace, C.N. (1990) Trends Biotechnol., 8, 93-98.
35. Palzkill, T. and Botstein, D. (1992) Proteins, 14, 29-44.
36. Plückthun, A. (1992) Immunol. Rev., 130, 151-188.
37. Plückthun, A. (1994) In van Oss, C.J. and van Regenmortel, M.H.V. (eds), Immunochemistry. Marcel Dekker, New York, pp. 201-235.
38. Plückthun, A., Krebber, A., Krebber, C., Horn, U., Knüpfer, U., Wenderoth, R., Nieba, L., Proba, K. and Riesenberg, D. (1996) In McCafferty, J. (ed.), Antibody Engineering. A Practical Approach. IRL Press, Oxford, pp. 203-252.
39. Prickett, K.S., Amberg, D.C. and Hopp, T.P. (1989) BioTechniques, 7, 580-589.
40. Prodromou, C. and Pearl, L.H. (1992) Protein Engng, 5, 827-829.
41. Spinelli, S., Frenken, L., Bourgeois, D., Ron, L., Bos, W., Verrips, T., Anguille, C., Cambillau, C. and Tegoni, M. (1996) Nature Struct. Biol., 3, 752-757.
42. Stemmer, W.P.C. (1994) Nature, 370, 389-391.
43. Stevenson, C.L. and Hageman, M.J. (1995) Pharm. Res., 12, 1671-1676.
44. Studier, F.W. and Moffatt, B.A. (1986) J. Mol. Biol., 189, 113-130.
45. Tramontano, A., Chothia, C. and Lesk, A. M. (1990) J. Mol. Biol., 215, 175-182.
46. Ullrich, H.D., Patten, P.A., Yang, P.L., Romesberg, F.E. and Schultz, P.G. (1995) Proc. Natl Acad. Sci. USA, 92, 11907-11911.
47. VanBogelen, R.A., Sankar, P., Clark, R.L., Bogan, J.A. and Neidhardt, F.C. (1992) Electrophoresis, 13, 1014-1054.
48. Van Wielink, J.E. and Duine, J.A. (1990) Trends Biochem. Sci., 15, 136-137.
49. Ward, E.S., Güssow, D., Griffiths, A.D., Jones, P.T. and Winter, G. (1989) Nature, 341, 544-546.
50. Whitlow, M. et al. (1993) Protein Engng, 6, 989-995.
51. Whitlow, M., Filupa, D., Rollence, M.L., Feng, S. and Wood, J.F. (1994) Protein Engng, 7, 1017-1026.
52. Whitlow, M., Howard, A.J., Wood, J.F., Voss, E.W., Jr and Hardman, K.D. (1995) Protein Engng, 8, 749-761.
53. Wilson, I.A. and Stanfield, R.L. (1994) Curr. Opin. Struct. Biol., 4, 857-867.
54. Winter, G. and Milstein, C. (1991) Nature, 349, 293-299.
55. Wu, A.M. et al. (1996) Immunotechnology, 2, 21-36.
56. Young, N.M., MacKenzie, C.R., Narang, S.A., Oomen, R.P. and Baenziger, J.E. (1995) FEBS Lett., 377, 135-139.