Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture

Biotechnology Progress

Volumn 18, Issue 2, 2002, Pages 212-220

  Yeung, Yik A ^a   Wittrup, K Dane ^a,b  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MAGNETIC BEAD STRUCTURE;

FLUORESCENCE; MUTAGENESIS; POLYPEPTIDES; PROBABILITY; YEAST;

BIOTECHNOLOGY;

ANTIBODY; LIGAND; MICROSPHERE; PEPTIDE LIBRARY; STREPTAVIDIN;

ANTIBODY AFFINITY; ARTICLE; COMPARATIVE STUDY; FEASIBILITY STUDY; FLOW CYTOMETRY; GENETICS; GROWTH, DEVELOPMENT AND AGING; MAGNETISM; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTATION; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY;

ANTIBODIES; ANTIBODY AFFINITY; FEASIBILITY STUDIES; FLOW CYTOMETRY; LIGANDS; MAGNETICS; MICROSPHERES; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDE LIBRARY; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SENSITIVITY AND SPECIFICITY; STREPTAVIDIN;

EID: 0036010267     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp010186l     Document Type: Article

References (32)

1. Biosynthetic polypeptide libraries
2. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface
3. Phage antibodies: Filamentous phage displaying antibody variable domains
4. Phage Display: Applications, innovations, and issues in phage and host biology
5. Strategies for selection of antibody by phage display
6. Transport and anchoring of β-lactamase to the external surface of Escherichia coli
7. Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines
8. Yeast surface display for screening combinatorial polypeptide libraries
9. By-passing immunization Human antibodies from V-gene libraries displayed on phage
10. Selection of phage antibodies by binding affinity mimicking affinity maturation
11. The contribution of contact and noncontact residues of antibody in the affinity of binding to antigen
12. Isolation of high affinity human antibodies directly from large synthetic repertoires
13. Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface
14. Antibody affinity maturation using bacterial surface display
15. Selection of functional T cell receptor mutants from a yeast surface-display library
16. Sequence requirements of the GPNG β-turn of the Ecballium elaterium trypsin inhibitor II explored by combinatorial library screening
17. The cystine knot of a squash-type protease inhibitor as a structural scaffold for Escherichia coli cell surface display of conformally constrainted peptides
18. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity
19. Flow cytometric screening of cell-based libraries
20. Commercial high-speed machines open new opportunities in high throughput flow cytometry (HTFC)
21. Use of magnetic techniques for the isolation of cells
22. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
23. Fine affinity discrimination by yeast surface display and flow cytometry
24. Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments
25. Constitutive overexpression of secreted heterologous proteins decreases extractable BiP and protein disulfide isomerase levels in Saccharomyces cerevisiae
26. Optimal screening of surface-displayed polypeptide libraries
27. Models for the specific adhesion of cells to cells
28. Estimate of the sticking probability for cells in uniform shear flow with adhesion caused by specific bonds
29. Concanavalin-A-mediated thymocyte agglutination: A model for a quantitative study of cell adhesion
30. Probabilistic modeling of shear-induced formation and breakage of doublets cross-linked by receptor-ligand bonds
31. Theoretical analysis of cell separation based on cell surface marker density
32. Making antibody fragments using phage display libraries