Highly sensitive detection of mutations in CHO cell recombinant DNA using multi-parallel single molecule real-time DNA sequencing

Biotechnology and Bioengineering

Volumn 115, Issue 6, 2018, Pages 1485-1498

  Cartwright, Joseph F ^a   Anderson, Karin ^b   Longworth, Joseph ^a   Lobb, Philip ^c   James, David C ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b PFIZER INC   (United States)

^c PACIFIC BIOSCIENCES   (United States)

Author keywords

CHO cells; next generation sequencing; sequence variants; single molecule real time sequencing

Indexed keywords

CELL CULTURE; CELL PROLIFERATION; DNA SEQUENCES; ENCODING (SYMBOLS); GENE ENCODING; GENE EXPRESSION; GENETIC ENGINEERING; MAMMALS; QUALITY ASSURANCE; RECOMBINANT PROTEINS; SIGNAL ENCODING;

CHO CELL; HIGHLY SENSITIVE DETECTIONS; MAMMALIAN CELL CULTURE; NEXT-GENERATION SEQUENCING; QUANTITATIVE ASSESSMENTS; REAL TIME; RECOMBINANT GENE EXPRESSIONS; SEQUENCE VARIANTS;

DNA;

PLASMID DNA; RECOMBINANT DNA; BIOLOGICAL PRODUCT; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

AMPLICON; ANIMAL CELL; ARTICLE; CELL POPULATION; CHO CELL LINE; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; FEMALE; GENE MUTATION; GENETIC DRIFT; MUTATION RATE; NATURAL SELECTION; NEXT GENERATION SEQUENCING; NONHUMAN; OPEN READING FRAME; PLASMID; POINT MUTATION; POLYMERASE CHAIN REACTION; SECOND GENERATION SEQUENCING; SELECTION BIAS; SEQUENCE ANALYSIS; ANIMAL; BIOLOGY; CRICETULUS; GENETICS; METABOLISM; MUTATION; PHARMACEUTICS; PROCEDURES;

ANIMALS; BIOLOGICAL PRODUCTS; CHO CELLS; COMPUTATIONAL BIOLOGY; CRICETULUS; DNA, RECOMBINANT; GREEN FLUORESCENT PROTEINS; MUTATION; PLASMIDS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TECHNOLOGY, PHARMACEUTICAL;

EID: 85042493294     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26561     Document Type: Article

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