Detection and identification of a serine to arginine sequence variant in a therapeutic monoclonal antibody

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 879, Issue 27, 2011, Pages 2877-2884

  Ren, Diya ^a   Zhang, Jian ^a   Pritchett, Ross ^a   Liu, Hongbin ^a   Kyauk, Jennifer ^a   Luo, Jun ^a   Amanullah, Ashraf ^a  

^a GENENTECH INC   (United States)

Author keywords

HPLC UV MS MS; Imaged capillary isoelectric focusing; Monoclonal antibody; Peptide mapping; Sequence variant

Indexed keywords

AMINO ACID SUBSTITUTION; ANALYTICAL METHOD; BIOPROCESS DEVELOPMENT; CAPILLARY ISOELECTRIC FOCUSING; CHINESE HAMSTER OVARY CELLS; CRITICAL QUALITY; EXTRACTED ION CHROMATOGRAM; HEAVY CHAIN; HPLC-UV; ION-EXCHANGE CHROMATOGRAPHY; MASS DETERMINATION; MONOCLONAL ANTIBODIES (MABS); PEPTIDE MAPPING; PRIMARY STRUCTURES; PROCESS DEVELOPMENT; PRODUCT QUALITY; SEQUENCE VARIANTS; SYNTHETIC PEPTIDE; THERAPEUTIC MONOCLONAL ANTIBODIES;

ARGININE; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ELECTROPHORESIS; ION EXCHANGE; MONOCLONAL ANTIBODIES; PEPTIDES;

CHEMICAL DETECTION;

ARGININE; IMMUNOGLOBULIN G1 ANTIBODY; SERINE;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CAPILLARY ISOELECTRIC FOCUSING; CHO CELL; HEAVY CHAIN; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; LIGHT CHAIN; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN POLYMORPHISM; TANDEM MASS SPECTROMETRY; ULTRAVIOLET SPECTROPHOTOMETRY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, MONOCLONAL; ARGININE; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAINS; METALLOENDOPEPTIDASES; PEPTIDE MAPPING; SEQUENCE ANALYSIS, PROTEIN; SERINE; TANDEM MASS SPECTROMETRY; TRYPSIN;

CRICETULUS GRISEUS;

EID: 80052930496     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2011.08.015     Document Type: Article

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