Discovery and investigation of misincorporation of serine at asparagine positions in recombinant proteins expressed in Chinese hamster ovary cells

Journal of Biological Chemistry

Volumn 284, Issue 47, 2009, Pages 32686-32694

  Wen, Dingyi ^a   Vecchi, Malgorzata M ^a   Gu, Sheng ^a   Su, Lihe ^a   Dolnikova, Jana ^a   Huang, Yao Ming ^a   Foley, Susan F ^a   Garber, Ellen ^a   Pederson, Nels ^a   Meier, Werner ^a  

^a BIOGEN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE MEDIUMS; CHINESE HAMSTER OVARY CELLS; MAMMALIAN CELLS; MASS MEASUREMENTS; PEPTIDE MAPPING; PROTEIN MOLECULES; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN PRODUCTIONS; SITE-SPECIFIC; TANDEM MASS SPECTROSCOPY;

AMINATION; AMINES; AMINO ACIDS; ANIMAL CELL CULTURE; CELL CULTURE; ESCHERICHIA COLI; MAMMALS; MASS SPECTROMETRY; NUCLEIC ACIDS; ORGANIC ACIDS;

PROTEINS;

ASPARAGINE; RECOMBINANT PROTEIN; SERINE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHO CELL; CODON; CONTROLLED STUDY; MOLECULAR WEIGHT; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PEPTIDE MAPPING; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY;

ANIMALS; ASPARAGINE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; ESCHERICHIA COLI; GLYCOSYLATION; MASS SPECTROMETRY; MODELS, BIOLOGICAL; PEPTIDE MAPPING; PEPTIDES; RECOMBINANT PROTEINS; RNA, TRANSFER; SERINE;

CRICETULUS GRISEUS; ESCHERICHIA COLI; MAMMALIA;

EID: 70450253191     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.059360     Document Type: Article

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