메뉴 건너뛰기




Volumn 48, Issue , 2017, Pages 21-27

Breaking the silence: new strategies for discovering novel natural products

Author keywords

[No Author keywords available]

Indexed keywords

ANTICANCER DRUG; BIOSYNTHETIC GENE CLUSTER; COMPUTATIONAL TOOLS; HIGH-THROUGHPUT METHOD; LABORATORY CONDITIONS; NATURAL PRODUCTS;

EID: 85014833240     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2017.02.008     Document Type: Review
Times cited : (91)

References (61)
  • 1
    • 84962367258 scopus 로고    scopus 로고
    • Natural products as sources of new drugs from 1981 to 2014
    • 1 Newman, D.J., Cragg, G.M., Natural products as sources of new drugs from 1981 to 2014. J Nat Prod 79 (2016), 629–661.
    • (2016) J Nat Prod , vol.79 , pp. 629-661
    • Newman, D.J.1    Cragg, G.M.2
  • 2
    • 34548108138 scopus 로고    scopus 로고
    • Targeting virulence: a new paradigm for antimicrobial therapy
    • 2 Clatworthy, A.E., Pierson, E., Hung, D.T., Targeting virulence: a new paradigm for antimicrobial therapy. Nat Chem Biol 3 (2007), 541–548.
    • (2007) Nat Chem Biol , vol.3 , pp. 541-548
    • Clatworthy, A.E.1    Pierson, E.2    Hung, D.T.3
  • 3
    • 84901809320 scopus 로고    scopus 로고
    • Antimicrobial Resistance: Global Report on Surveillance
    • World Health Organization
    • 3 World Health Organization, Antimicrobial Resistance: Global Report on Surveillance., 2014, World Health Organization.
    • (2014)
    • World Health Organization1
  • 5
    • 84937253653 scopus 로고    scopus 로고
    • Discovery of microbial natural products by activation of silent biosynthetic gene clusters
    • This is a comprehensive review on various approaches for activating silent BGCs.
    • 5•• Rutledge, P.J., Challis, G.L., Discovery of microbial natural products by activation of silent biosynthetic gene clusters. Nat Rev Microbiol 13 (2015), 509–523 This is a comprehensive review on various approaches for activating silent BGCs.
    • (2015) Nat Rev Microbiol , vol.13 , pp. 509-523
    • Rutledge, P.J.1    Challis, G.L.2
  • 6
    • 84938244327 scopus 로고    scopus 로고
    • Small molecules from the human microbiota
    • 6 Donia, M.S., Fischbach, M.A., Small molecules from the human microbiota. Science, 349, 2015, 1254766.
    • (2015) Science , vol.349 , pp. 1254766
    • Donia, M.S.1    Fischbach, M.A.2
  • 7
    • 84952705410 scopus 로고    scopus 로고
    • Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products
    • 7 Zarins-Tutt, J.S., Barberi, T.T., Gao, H., Mearns-Spragg, A., Zhang, L., Newman, D.J., Goss, R.J.M., Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products. J Nat Prod 33 (2016), 54–72.
    • (2016) J Nat Prod , vol.33 , pp. 54-72
    • Zarins-Tutt, J.S.1    Barberi, T.T.2    Gao, H.3    Mearns-Spragg, A.4    Zhang, L.5    Newman, D.J.6    Goss, R.J.M.7
  • 8
    • 85014230646 scopus 로고    scopus 로고
    • The secondary metabolite bioinformatics portal: computational tools to facilitate synthetic biology of secondary metabolite production
    • 8 Weber, T., Kim, H.U., The secondary metabolite bioinformatics portal: computational tools to facilitate synthetic biology of secondary metabolite production. Synth Syst Biotechnol 1 (2016), 69–79.
    • (2016) Synth Syst Biotechnol , vol.1 , pp. 69-79
    • Weber, T.1    Kim, H.U.2
  • 11
    • 84874850153 scopus 로고    scopus 로고
    • Molecular regulation of antibiotic biosynthesis in Streptomyces
    • 11 Liu, G., Chater, K.F., Chandra, G., Niu, G., Tan, H., Molecular regulation of antibiotic biosynthesis in Streptomyces. Microbiol Mol Biol Rev 77 (2013), 112–143.
    • (2013) Microbiol Mol Biol Rev , vol.77 , pp. 112-143
    • Liu, G.1    Chater, K.F.2    Chandra, G.3    Niu, G.4    Tan, H.5
  • 13
    • 84940000692 scopus 로고    scopus 로고
    • Identification of AstG1, a LAL family regulator that positively controls ansatrienins production in Streptomyces sp. XZQH13
    • 13 Xie, C., Deng, J.J., Wang, H.X., Identification of AstG1, a LAL family regulator that positively controls ansatrienins production in Streptomyces sp. XZQH13. Curr Microbiol 70 (2015), 859–864.
    • (2015) Curr Microbiol , vol.70 , pp. 859-864
    • Xie, C.1    Deng, J.J.2    Wang, H.X.3
  • 14
    • 84922311253 scopus 로고    scopus 로고
    • Genome mining-directed activation of a silent angucycline biosynthetic gene cluster in Streptomyces chattanoogensis
    • 14 Zhou, Z.X., Xu, Q.Q., Bu, Q.T., Guo, Y.Y., Liu, S.P., Liu, Y., Du, Y.L., Li, Y.Q., Genome mining-directed activation of a silent angucycline biosynthetic gene cluster in Streptomyces chattanoogensis. ChemBioChem 16 (2015), 496–502.
    • (2015) ChemBioChem , vol.16 , pp. 496-502
    • Zhou, Z.X.1    Xu, Q.Q.2    Bu, Q.T.3    Guo, Y.Y.4    Liu, S.P.5    Liu, Y.6    Du, Y.L.7    Li, Y.Q.8
  • 15
    • 84938875734 scopus 로고    scopus 로고
    • Activating a cryptic ansamycin biosynthetic gene cluster to produce three new naphthalenic octaketide ansamycins with n-pentyl and n-butyl side chains
    • 15 Li, S.R., Li, Y.Y., Lu, C.H., Zhang, J.L., Zhu, J., Wang, H.X., Shen, Y.M., Activating a cryptic ansamycin biosynthetic gene cluster to produce three new naphthalenic octaketide ansamycins with n-pentyl and n-butyl side chains. Org Lett 17 (2015), 3706–3709.
    • (2015) Org Lett , vol.17 , pp. 3706-3709
    • Li, S.R.1    Li, Y.Y.2    Lu, C.H.3    Zhang, J.L.4    Zhu, J.5    Wang, H.X.6    Shen, Y.M.7
  • 16
    • 84921496414 scopus 로고    scopus 로고
    • Targeted activation of silent natural product biosynthesis pathways by reporter-guided mutant selection
    • This work alied the reporter method for activation of silent gene clusters and used a double-reporter system, xylE-neo, to lower the false postive rate dramatically. In principle, the target cluster activated mutants can be directly selelcted after the double-reporter screening.
    • 16• Guo, F., Xiang, S.H., Li, L.Y., Wang, B., Rajasarkka, J., Grondahl-Yli-Hannuksela, K., Ai, G.M., Metsa-Ketela, M., Yang, K.G., Targeted activation of silent natural product biosynthesis pathways by reporter-guided mutant selection. Metab Eng 28 (2015), 134–142 This work alied the reporter method for activation of silent gene clusters and used a double-reporter system, xylE-neo, to lower the false postive rate dramatically. In principle, the target cluster activated mutants can be directly selelcted after the double-reporter screening.
    • (2015) Metab Eng , vol.28 , pp. 134-142
    • Guo, F.1    Xiang, S.H.2    Li, L.Y.3    Wang, B.4    Rajasarkka, J.5    Grondahl-Yli-Hannuksela, K.6    Ai, G.M.7    Metsa-Ketela, M.8    Yang, K.G.9
  • 17
    • 84941686655 scopus 로고    scopus 로고
    • Identification of novel mureidomycin analogues via rational activation of a cryptic gene cluster in Streptomyces roseosporus NRRL 15998
    • 17 Jiang, L.J., Wang, L., Zhang, J.H., Liu, H., Hong, B., Tan, H.R., Niu, G.Q., Identification of novel mureidomycin analogues via rational activation of a cryptic gene cluster in Streptomyces roseosporus NRRL 15998. Sci Rep, 5, 2015, 14111, 10.11038/srep14111.
    • (2015) Sci Rep , vol.5 , pp. 14111
    • Jiang, L.J.1    Wang, L.2    Zhang, J.H.3    Liu, H.4    Hong, B.5    Tan, H.R.6    Niu, G.Q.7
  • 19
    • 84883137478 scopus 로고    scopus 로고
    • VeA and MvlA repression of the cryptic orsellinic acid gene cluster in Aspergillus nidulans involves histone 3 acetylation
    • 19 Bok, J.W., Soukup, A.A., Chadwick, E., Chiang, Y.M., Wang, C.C.C., Keller, N.P., VeA and MvlA repression of the cryptic orsellinic acid gene cluster in Aspergillus nidulans involves histone 3 acetylation. Mol Microbiol 89 (2013), 963–974.
    • (2013) Mol Microbiol , vol.89 , pp. 963-974
    • Bok, J.W.1    Soukup, A.A.2    Chadwick, E.3    Chiang, Y.M.4    Wang, C.C.C.5    Keller, N.P.6
  • 20
    • 84934947770 scopus 로고    scopus 로고
    • High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system
    • 20 Cobb, R.E., Wang, Y.J., Zhao, H.M., High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system. ACS Synth Biol 4 (2015), 723–728.
    • (2015) ACS Synth Biol , vol.4 , pp. 723-728
    • Cobb, R.E.1    Wang, Y.J.2    Zhao, H.M.3
  • 21
    • 85017266739 scopus 로고    scopus 로고
    • An efficient CRISPR-Cas9 mediated knock-in strategy for activation of silent Streptomyces biosynthetic gene clusters for natural product discovery
    • This work applied the CRISPR-Cas9 system based promoter knockin strategy to activate multiple silent BGCs in five different  Streptomyces species, demonstrating a great potential for high throughput discovery of natural products in the native hosts.
    • 21•• Zhang, M., Wong, F., Wang, Y., Luo, S., Lim, Y., Heng, E., Yeo, W., Cobb, R., Enghiad, B., Ang, E., et al. An efficient CRISPR-Cas9 mediated knock-in strategy for activation of silent Streptomyces biosynthetic gene clusters for natural product discovery. Nat Chem Biol, 2017, 10.1038/nchembio.2341 This work applied the CRISPR-Cas9 system based promoter knockin strategy to activate multiple silent BGCs in five different  Streptomyces species, demonstrating a great potential for high throughput discovery of natural products in the native hosts.
    • (2017) Nat Chem Biol
    • Zhang, M.1    Wong, F.2    Wang, Y.3    Luo, S.4    Lim, Y.5    Heng, E.6    Yeo, W.7    Cobb, R.8    Enghiad, B.9    Ang, E.10
  • 22
    • 84940106526 scopus 로고    scopus 로고
    • CRISPR-Cas9 based engineering of actinomycetal genomes
    • 22 Tong, Y., Charusanti, P., Zhang, L., Weber, T., Lee, S.Y., CRISPR-Cas9 based engineering of actinomycetal genomes. ACS Synth Biol 4 (2015), 1020–1029.
    • (2015) ACS Synth Biol , vol.4 , pp. 1020-1029
    • Tong, Y.1    Charusanti, P.2    Zhang, L.3    Weber, T.4    Lee, S.Y.5
  • 23
    • 84952803196 scopus 로고    scopus 로고
    • In vitro CRISPR/Cas9 system for efficient targeted DNA editing
    • 23 Liu, Y., Tao, W., Wen, S., Li, Z., Yang, A., Deng, Z., Sun, Y., In vitro CRISPR/Cas9 system for efficient targeted DNA editing. mBio 6 (2015), e01714–e01715.
    • (2015) mBio , vol.6 , pp. e01714-e01715
    • Liu, Y.1    Tao, W.2    Wen, S.3    Li, Z.4    Yang, A.5    Deng, Z.6    Sun, Y.7
  • 24
    • 84987909256 scopus 로고    scopus 로고
    • Multiplexed CRISPR/Cas9- and TAR-mediated promoter engineering of natural product biosynthetic gene clusters in yeast
    • This work combined CRISPR/Cas9 and TAR to enable efficient large gene cluster assembly and multiplexed promoter engineering in a single round. It also provides a mCRISTAR python package and Webapp for transcriptional activation of complex natural product BGCs.
    • 24• Kang, H.S., Charlop-Powers, Z., Brady, S.F., Multiplexed CRISPR/Cas9- and TAR-mediated promoter engineering of natural product biosynthetic gene clusters in yeast. ACS Synth Biol 5 (2016), 1002–1010 This work combined CRISPR/Cas9 and TAR to enable efficient large gene cluster assembly and multiplexed promoter engineering in a single round. It also provides a mCRISTAR python package and Webapp for transcriptional activation of complex natural product BGCs.
    • (2016) ACS Synth Biol , vol.5 , pp. 1002-1010
    • Kang, H.S.1    Charlop-Powers, Z.2    Brady, S.F.3
  • 26
    • 84960898238 scopus 로고    scopus 로고
    • Technology development for natural product biosynthesis in Saccharomyces cerevisiae
    • 26 Billingsley, J.M., DeNicola, A.B., Tang, Y., Technology development for natural product biosynthesis in Saccharomyces cerevisiae. Curr Opin Biotechnol 42 (2016), 74–83.
    • (2016) Curr Opin Biotechnol , vol.42 , pp. 74-83
    • Billingsley, J.M.1    DeNicola, A.B.2    Tang, Y.3
  • 27
    • 0036902202 scopus 로고    scopus 로고
    • Big effects from small changes: possible ways to explore nature's chemical diversity
    • 27 Bode, H.B., Bethe, B., Höfs, R., Zeeck, A., Big effects from small changes: possible ways to explore nature's chemical diversity. ChemBioChem 3 (2002), 619–627.
    • (2002) ChemBioChem , vol.3 , pp. 619-627
    • Bode, H.B.1    Bethe, B.2    Höfs, R.3    Zeeck, A.4
  • 29
    • 84893449419 scopus 로고    scopus 로고
    • Activating the expression of bacterial cryptic genes by rpoB mutations in RNA polymerase or by rare earth elements
    • 29 Ochi, K., Tanaka, Y., Tojo, S., Activating the expression of bacterial cryptic genes by rpoB mutations in RNA polymerase or by rare earth elements. J Ind Microbiol Biotechnol 41 (2014), 403–414.
    • (2014) J Ind Microbiol Biotechnol , vol.41 , pp. 403-414
    • Ochi, K.1    Tanaka, Y.2    Tojo, S.3
  • 30
    • 85014812917 scopus 로고    scopus 로고
    • Antibiotic dialogues: induction of silent biosynthetic gene clusters by exogenous small molecules
    • 30 Okada, B.K., Seyedsayamdost, M.R., Antibiotic dialogues: induction of silent biosynthetic gene clusters by exogenous small molecules. FEMS Microbiol Rev 41 (2017), 19–33.
    • (2017) FEMS Microbiol Rev , vol.41 , pp. 19-33
    • Okada, B.K.1    Seyedsayamdost, M.R.2
  • 31
    • 84901000640 scopus 로고    scopus 로고
    • High-throughput platform for the discovery of elicitors of silent bacterial gene clusters
    • 31 Seyedsayamdost, M.R., High-throughput platform for the discovery of elicitors of silent bacterial gene clusters. Proc Natl Acad Sci U S A 111 (2014), 7266–7271.
    • (2014) Proc Natl Acad Sci U S A , vol.111 , pp. 7266-7271
    • Seyedsayamdost, M.R.1
  • 32
    • 0030040287 scopus 로고    scopus 로고
    • Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination
    • 32 Larionov, V., Kouprina, N., Graves, J., Chen, X.N., Korenberg, J.R., Resnick, M.A., Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination. Proc Natl Acad Sci U S A 93 (1996), 491–496.
    • (1996) Proc Natl Acad Sci U S A , vol.93 , pp. 491-496
    • Larionov, V.1    Kouprina, N.2    Graves, J.3    Chen, X.N.4    Korenberg, J.R.5    Resnick, M.A.6
  • 33
    • 40649092126 scopus 로고    scopus 로고
    • Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae
    • 33 Kouprina, N., Larionov, V., Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae. Nat Protoc 3 (2008), 371–377.
    • (2008) Nat Protoc , vol.3 , pp. 371-377
    • Kouprina, N.1    Larionov, V.2
  • 34
    • 84893456760 scopus 로고    scopus 로고
    • Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A
    • This is the first application of TAR in natural product discovery.
    • 34•• Yamanaka, K., Reynolds, K.A., Kersten, R.D., Ryan, K.S., Gonzalez, D.J., Nizet, V., Dorrestein, P.C., Moore, B.S., Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc Natl Acad Sci U S A 111 (2014), 1957–1962 This is the first application of TAR in natural product discovery.
    • (2014) Proc Natl Acad Sci U S A , vol.111 , pp. 1957-1962
    • Yamanaka, K.1    Reynolds, K.A.2    Kersten, R.D.3    Ryan, K.S.4    Gonzalez, D.J.5    Nizet, V.6    Dorrestein, P.C.7    Moore, B.S.8
  • 35
    • 84926298894 scopus 로고    scopus 로고
    • Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis
    • 35 Li, Y.X., Li, Z.R., Yamanaka, K., Xu, Y., Zhang, W.P., Vlamakis, H., Kolter, R., Moore, B.S., Qian, P.Y., Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis. Sci Rep, 5, 2015, 9383.
    • (2015) Sci Rep , vol.5 , pp. 9383
    • Li, Y.X.1    Li, Z.R.2    Yamanaka, K.3    Xu, Y.4    Zhang, W.P.5    Vlamakis, H.6    Kolter, R.7    Moore, B.S.8    Qian, P.Y.9
  • 36
    • 84928165512 scopus 로고    scopus 로고
    • Targeted capture and heterologous expression of the Pseudoalteromonas alterochromide gene cluster in Escherichia coli represents a promising natural product exploratory platform
    • 36 Ross, A.C., Gulland, L.E.S., Dorrestein, P.C., Moore, B.S., Targeted capture and heterologous expression of the Pseudoalteromonas alterochromide gene cluster in Escherichia coli represents a promising natural product exploratory platform. ACS Synth Biol 4 (2015), 414–420.
    • (2015) ACS Synth Biol , vol.4 , pp. 414-420
    • Ross, A.C.1    Gulland, L.E.S.2    Dorrestein, P.C.3    Moore, B.S.4
  • 38
    • 0031924072 scopus 로고    scopus 로고
    • Use of bacteriophage l recombination functions to promote gene replacement in Escherichia coli
    • 38 Murphy, K.C., Use of bacteriophage l recombination functions to promote gene replacement in Escherichia coli. J Bacteriol 180 (1998), 2063–2071.
    • (1998) J Bacteriol , vol.180 , pp. 2063-2071
    • Murphy, K.C.1
  • 39
    • 0031664853 scopus 로고    scopus 로고
    • A new logic for DNA engineering using recombination in Escherichia coli
    • 39 Zhang, Y., Buchholz, F., Muyrers, J.P., Stewart, aF., A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 20 (1998), 123–128.
    • (1998) Nat Genet , vol.20 , pp. 123-128
    • Zhang, Y.1    Buchholz, F.2    Muyrers, J.P.3    Stewart, A.F.4
  • 40
    • 84860778298 scopus 로고    scopus 로고
    • Full-length RecE enhances linear–linear homologous recombination and facilitates direct cloning for bioprospecting
    • 40 Fu, J., Bian, X.Y., Hu, S.B., Wang, H.L., Huang, F., Seibert, P.M., Plaza, A., Xia, L.Q., Muller, R., Stewart, A.F., et al. Full-length RecE enhances linear–linear homologous recombination and facilitates direct cloning for bioprospecting. Nat Biotechnol 30 (2012), 440–446.
    • (2012) Nat Biotechnol , vol.30 , pp. 440-446
    • Fu, J.1    Bian, X.Y.2    Hu, S.B.3    Wang, H.L.4    Huang, F.5    Seibert, P.M.6    Plaza, A.7    Xia, L.Q.8    Muller, R.9    Stewart, A.F.10
  • 41
    • 84958214556 scopus 로고    scopus 로고
    • Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering
    • 41 Tu, Q., Herrmann, J., Hu, S., Raju, R., Bian, X., Zhang, Y., Müller, R., Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering. Sci Rep, 6, 2016, 21066.
    • (2016) Sci Rep , vol.6 , pp. 21066
    • Tu, Q.1    Herrmann, J.2    Hu, S.3    Raju, R.4    Bian, X.5    Zhang, Y.6    Müller, R.7
  • 42
    • 84944249797 scopus 로고    scopus 로고
    • Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2)
    • 42 Yin, J., Hoffmann, M., Bian, X., Tu, Q., Yan, F., Xia, L., Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2). Sci Rep, 5, 2015, 15081.
    • (2015) Sci Rep , vol.5 , pp. 15081
    • Yin, J.1    Hoffmann, M.2    Bian, X.3    Tu, Q.4    Yan, F.5    Xia, L.6
  • 43
    • 84919934346 scopus 로고    scopus 로고
    • Heterologous expression of an orphan NRPS gene cluster from Paenibacillus larvae in Escherichia coli revealed production of sevadicin
    • 43 Tang, Y., Frewert, S., Harmrolfs, K., Herrmann, J., Karmann, L., Kazmaier, U., Xia, L., Zhang, Y., Müller, R., Heterologous expression of an orphan NRPS gene cluster from Paenibacillus larvae in Escherichia coli revealed production of sevadicin. J Biotechnol 194 (2015), 112–114.
    • (2015) J Biotechnol , vol.194 , pp. 112-114
    • Tang, Y.1    Frewert, S.2    Harmrolfs, K.3    Herrmann, J.4    Karmann, L.5    Kazmaier, U.6    Xia, L.7    Zhang, Y.8    Müller, R.9
  • 44
    • 84975885993 scopus 로고    scopus 로고
    • RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression
    • 44 Wang, H., Li, Z., Jia, R., Hou, Y., Yin, J., Bian, X., Li, A., Müller, R., Stewart, A.F., Fu, J., et al. RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression. Nat Protoc 11 (2016), 1175–1190.
    • (2016) Nat Protoc , vol.11 , pp. 1175-1190
    • Wang, H.1    Li, Z.2    Jia, R.3    Hou, Y.4    Yin, J.5    Bian, X.6    Li, A.7    Müller, R.8    Stewart, A.F.9    Fu, J.10
  • 45
    • 84983535014 scopus 로고    scopus 로고
    • Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments
    • 45 Jiang, W., Zhu, T.F., Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments. Nat Protoc 11 (2016), 960–975.
    • (2016) Nat Protoc , vol.11 , pp. 960-975
    • Jiang, W.1    Zhu, T.F.2
  • 46
    • 84940676093 scopus 로고    scopus 로고
    • Cas9-assisted targeting of chromosome segments CATCH enables one-step targeted cloning of large gene clusters
    • This paper describes a direct cloning method based on the in vitro cleavage of a target BGC from genomic DNA using CRISPR/Cas9.
    • 46• Jiang, W.J., Zhao, X.J., Gabrieli, T., Lou, C.B., Ebenstein, Y., Zhu, T.F., Cas9-assisted targeting of chromosome segments CATCH enables one-step targeted cloning of large gene clusters. Nat Commun, 6, 2015, 6101 This paper describes a direct cloning method based on the in vitro cleavage of a target BGC from genomic DNA using CRISPR/Cas9.
    • (2015) Nat Commun , vol.6 , pp. 6101
    • Jiang, W.J.1    Zhao, X.J.2    Gabrieli, T.3    Lou, C.B.4    Ebenstein, Y.5    Zhu, T.F.6
  • 47
    • 84976869995 scopus 로고    scopus 로고
    • “Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens
    • 47 Hu, S., Liu, Z., Zhang, X., Zhang, G., Xie, Y., Ding, X., Mo, X., Stewart, A.F., Fu, J., Zhang, Y., et al. “Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens. Sci Rep, 6, 2016, 29087.
    • (2016) Sci Rep , vol.6 , pp. 29087
    • Hu, S.1    Liu, Z.2    Zhang, X.3    Zhang, G.4    Xie, Y.5    Ding, X.6    Mo, X.7    Stewart, A.F.8    Fu, J.9    Zhang, Y.10
  • 48
    • 84941182011 scopus 로고    scopus 로고
    • Site-specific recombination for cloning of large DNA fragments in vitro
    • 48 Dai, R.X., Zhang, B., Zhao, G.P., Ding, X.M., Site-specific recombination for cloning of large DNA fragments in vitro. Eng Life Sci 15 (2015), 655–659.
    • (2015) Eng Life Sci , vol.15 , pp. 655-659
    • Dai, R.X.1    Zhang, B.2    Zhao, G.P.3    Ding, X.M.4
  • 49
    • 84937722803 scopus 로고    scopus 로고
    • Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters
    • This paper describes a HR-based method for replacing promoters of a silent BGC on the shuttle vector.
    • 49• Montiel, D., Kang, H.S., Chang, F.Y., Charlop-Powers, Z., Brady, S.F., Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters. Proc Natl Acad Sci U S A 112 (2015), 8953–8958 This paper describes a HR-based method for replacing promoters of a silent BGC on the shuttle vector.
    • (2015) Proc Natl Acad Sci U S A , vol.112 , pp. 8953-8958
    • Montiel, D.1    Kang, H.S.2    Chang, F.Y.3    Charlop-Powers, Z.4    Brady, S.F.5
  • 50
    • 84890086658 scopus 로고    scopus 로고
    • Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster
    • 50 Luo, Y., Huang, H., Liang, J., Wang, M., Lu, L., Shao, Z., Cobb, R.E., Zhao, H., Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster. Nat Commun, 4, 2013, 2894.
    • (2013) Nat Commun , vol.4 , pp. 2894
    • Luo, Y.1    Huang, H.2    Liang, J.3    Wang, M.4    Lu, L.5    Shao, Z.6    Cobb, R.E.7    Zhao, H.8
  • 51
    • 84887870749 scopus 로고    scopus 로고
    • Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold
    • 51 Shao, Z., Rao, G., Li, C., Abil, Z., Luo, Y., Zhao, H., Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold. ACS Synth Biol 2 (2013), 662–669.
    • (2013) ACS Synth Biol , vol.2 , pp. 662-669
    • Shao, Z.1    Rao, G.2    Li, C.3    Abil, Z.4    Luo, Y.5    Zhao, H.6
  • 52
    • 84937816186 scopus 로고    scopus 로고
    • Recent advances in DNA assembly technologies
    • 52 Chao, R., Yuan, Y., Zhao, H., Recent advances in DNA assembly technologies. FEMS Yeast Res 15 (2015), 1–9.
    • (2015) FEMS Yeast Res , vol.15 , pp. 1-9
    • Chao, R.1    Yuan, Y.2    Zhao, H.3
  • 53
    • 84979964893 scopus 로고    scopus 로고
    • Engineering microbial hosts for production of bacterial natural products
    • 53 Zhang, M.M., Wang, Y., Ang, E.L., Zhao, H., Engineering microbial hosts for production of bacterial natural products. Nat Prod Rep 33 (2016), 963–987.
    • (2016) Nat Prod Rep , vol.33 , pp. 963-987
    • Zhang, M.M.1    Wang, Y.2    Ang, E.L.3    Zhao, H.4
  • 54
    • 84942761231 scopus 로고    scopus 로고
    • An automated genomes-to-natural products platform (GNP) for the discovery of modular natural products
    • The authors of this paper established a genomes-to-natural products (GNP) platform for predicting, combinatorializing and identifying the products of modular gene clusters (PKS and NRPS) from LC–MS/MS data of crude extracts.
    • 54•• Johnston, C.W., Skinnider, M.A., Wyatt, M.A., Li, X., Ranieri, M.R.M., Yang, L., Zechel, D.L., Ma, B., Magarvey, N.A., An automated genomes-to-natural products platform (GNP) for the discovery of modular natural products. Nat Commun, 6, 2015, 8421 The authors of this paper established a genomes-to-natural products (GNP) platform for predicting, combinatorializing and identifying the products of modular gene clusters (PKS and NRPS) from LC–MS/MS data of crude extracts.
    • (2015) Nat Commun , vol.6 , pp. 8421
    • Johnston, C.W.1    Skinnider, M.A.2    Wyatt, M.A.3    Li, X.4    Ranieri, M.R.M.5    Yang, L.6    Zechel, D.L.7    Ma, B.8    Magarvey, N.A.9
  • 57
    • 84980018541 scopus 로고    scopus 로고
    • Modern mass spectrometry for synthetic biology and structure-based discovery of natural products
    • 57 Henke, M.T., Kelleher, N.L., Modern mass spectrometry for synthetic biology and structure-based discovery of natural products. Nat Prod Rep 33 (2016), 942–950.
    • (2016) Nat Prod Rep , vol.33 , pp. 942-950
    • Henke, M.T.1    Kelleher, N.L.2
  • 58
    • 84952673564 scopus 로고    scopus 로고
    • Dereplication, sequencing and identification of peptidic natural products: from genome mining to peptidogenomics to spectral networks
    • 58 Mohimani, H., Pevzner, P.A., Dereplication, sequencing and identification of peptidic natural products: from genome mining to peptidogenomics to spectral networks. J Nat Prod 33 (2015), 73–86.
    • (2015) J Nat Prod , vol.33 , pp. 73-86
    • Mohimani, H.1    Pevzner, P.A.2
  • 60
    • 84856217217 scopus 로고    scopus 로고
    • From single to multiple microcoil flow probe NMR and related capillary techniques: a review
    • 60 Gökay, O., Albert, K., From single to multiple microcoil flow probe NMR and related capillary techniques: a review. Anal Bioanal Chem 402 (2012), 647–669.
    • (2012) Anal Bioanal Chem , vol.402 , pp. 647-669
    • Gökay, O.1    Albert, K.2
  • 61


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.