Multiplexed CRISPR/Cas9- and TAR-Mediated Promoter Engineering of Natural Product Biosynthetic Gene Clusters in Yeast

ACS Synthetic Biology

Volumn 5, Issue 9, 2016, Pages 1002-1010

  Kang, Hahk Soo ^a,b   Charlop Powers, Zachary ^a   Brady, Sean F ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b Konkuk University   (South Korea)

Author keywords

CRISPR Cas9; natural products; promoter engineering; TAR

Indexed keywords

CRISPR ASSOCIATED PROTEIN; GUIDE RNA; NATURAL PRODUCT; BIOLOGICAL PRODUCT;

ARTICLE; AUXOTROPHY; BACTERIAL GENOME; BIOSYNTHESIS; CRISPR CAS SYSTEM; DOUBLE STRANDED DNA BREAK; GENE CLUSTER; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; HETEROLOGOUS EXPRESSION; METAGENOME; MICROBIAL GENOME; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; TRANSFORMATION ASSOCIATED RECOMBINATION; YEAST; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA SEQUENCE; GENE VECTOR; GENETICS; METABOLISM; MULTIGENE FAMILY; PLASMID; PROCEDURES;

BIOLOGICAL PRODUCTS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; GENETIC ENGINEERING; GENETIC VECTORS; GENOME, BACTERIAL; MULTIGENE FAMILY; PLASMIDS; PROMOTER REGIONS, GENETIC; SEQUENCE ANALYSIS, DNA; YEASTS;

EID: 84987909256     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00080     Document Type: Article

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