Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments

Nature Protocols

Volumn 11, Issue 5, 2016, Pages 960-975

  Jiang, Wenjun ^a   Zhu, Ting F ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; ENDONUCLEASE; GUIDE RNA;

ARTICLE; CAS9-ASSISTED TARGETING OF CHROMOSOME SEGMENTS; CHROMOSOME; CRISPR-CAS9 SYSTEM; ESCHERICHIA COLI; GENE CLUSTER; GENE SEQUENCE; GENETIC ENGINEERING; IN VITRO STUDY; MICROBIAL GENOME; MOLECULAR BIOLOGY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION MAPPING; SYNTHETIC BIOLOGY; TIME; BACTERIAL CHROMOSOME; BACTERIAL GENOME; GENE VECTOR; GENETICS; GENOMICS; PROCEDURES; WORKFLOW;

BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; ENDONUCLEASES; ESCHERICHIA COLI; GENETIC VECTORS; GENOME, BACTERIAL; GENOMICS; RNA, GUIDE; SYNTHETIC BIOLOGY; WORKFLOW;

EID: 84983535014     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.055     Document Type: Article

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