Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 5, 2014, Pages 1957-1962

  Yamanaka, Kazuya ^a,b   Reynolds, Kirk A ^a,c   Kersten, Roland D ^a   Ryan, Katherine S ^a,d   Gonzalez, David J ^c   Nizet, Victor ^c,e   Dorrestein, Pieter C ^a,c,e   Moore, Bradley S ^a,e  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CHISSO CORPORATION   (Japan)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^e UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

Biosynthesis; Drug discovery; Genome mining

Indexed keywords

DAPTOMYCIN; LIPOPEPTIDE; NONRIBOSOMAL PEPTIDE SYNTHETASE; POLYPEPTIDE ANTIBIOTIC AGENT; TAROMYCIN A; UNCLASSIFIED DRUG;

ACTINOBACTERIA; ARTICLE; BIOSYNTHESIS; CLONING VECTOR; DRUG STRUCTURE; GENE CLUSTER; GENETIC MANIPULATION; GENETIC RECOMBINATION; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; PRIORITY JOURNAL; REGULATOR GENE; SACCHAROMONOSPORA; STREPTOMYCES COELICOLOR; TRANSFORMATION ASSOCIATED RECOMBINATION;

BIOSYNTHESIS; DRUG DISCOVERY; GENOME MINING;

BIOSYNTHETIC PATHWAYS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DAPTOMYCIN; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, REGULATOR; GENETIC COMPLEMENTATION TEST; GENETIC VECTORS; LIPOPEPTIDES; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; PHYSICAL CHROMOSOME MAPPING; RECOMBINATION, GENETIC; REPRODUCIBILITY OF RESULTS; STREPTOMYCES;

EID: 84893456760     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1319584111     Document Type: Article

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