Comprehensive in vitro analysis of acyltransferase domain exchanges in modular polyketide synthases and its application for short-chain ketone production

ACS Synthetic Biology

Volumn 6, Issue 1, 2017, Pages 139-147

  Yuzawa, Satoshi ^a   Deng, Kai ^d,e   Wang, George ^d   Baidoo, Edward E K ^d   Northen, Trent R ^d,f   Adams, Paul D ^b,d,f   Katz, Leonard ^a,f   Keasling, Jay D ^a,b,c,d,g,h  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b University of California at Berkeley   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d JOINT BIOENERGY INSTITUTE   (United States)

^e SANDIA NATIONAL LABORATORIES   (United States)

^f LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^g Synthetic Biology Engineering Research Center   (United States)

^h Chalmers University of Technology   (Denmark)

Author keywords

Acyltransferase domain; Protein engineering; Substrate specificity; Synthetic biology tool; Type I modular polyketide synthase

Indexed keywords

ACYL COENZYME A; ACYLTRANSFERASE; KETONE; POLYKETIDE SYNTHASE; RECOMBINANT PROTEIN;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME SPECIFICITY; HYBRID; IN VITRO STUDY; IN VIVO STUDY; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STABILITY; SYNTHETIC BIOLOGY; AMINO ACID SEQUENCE; CHEMISTRY; GENETICS; METABOLISM; PROCEDURES; PROTEIN ENGINEERING;

ACYL COENZYME A; ACYLTRANSFERASES; AMINO ACID SEQUENCE; IN VITRO TECHNIQUES; KETONES; POLYKETIDE SYNTHASES; PROTEIN DOMAINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; SYNTHETIC BIOLOGY;

EID: 85012836622     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00176     Document Type: Article

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