A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies

Nature Protocols

Volumn 11, Issue 8, 2016, Pages 1554-1571

  Magnani, Francesca ^a,b   Serrano Vega, Maria J ^a,c   Shibata, Yoko ^a,d   Abdul Hussein, Saba ^a,e   Lebon, Guillaume ^a,f   Miller Gallacher, Jennifer ^a,g   Singhal, Ankita ^a   Strege, Annette ^a   Thomas, Jennifer A ^a   Tate, Christopher G ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b UNIVERSITY OF PAVIA   (Italy)

^c Heptares Therapeutics Ltd   (United Kingdom)

^d MEDIMMUNE LTD   (United Kingdom)

^e STOCKHOLM UNIVERSITY   (Sweden)

^f INSTITUT DE GÉNOMIQUE FONCTIONNELLE   (France)

^g RSR Limited   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; DETERGENT;

ARTICLE; BINDING AFFINITY; COMPARATIVE STUDY; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; GENETIC TRANSFECTION; ISOTOPE LABELING; LIGAND BINDING; METHODOLOGY; MUTAGENESIS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; POINT MUTATION; PRIORITY JOURNAL; PROCESS DESIGN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SCREENING TEST; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; CHEMISTRY; GENETICS; MOLECULAR MODEL; MUTATION; PROTEIN CONFORMATION; PROTEIN STABILITY; SOLUBILITY; TEMPERATURE;

AMINO ACID SEQUENCE; DETERGENTS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; PROTEIN CONFORMATION; PROTEIN STABILITY; SOLUBILITY; TEMPERATURE;

EID: 84979929935     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.088     Document Type: Article

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