A scalable, GFP-based pipeline for membrane protein overexpression screening and purification

Protein Science

Volumn 14, Issue 8, 2005, Pages 2011-2017

  Drew, David ^a   Slotboom, Dirk Jan ^b   Friso, Giulia ^c   Reda, Torsten ^b   Genevaux, Pierre ^d   Rapp, Mikaela ^a   Meindl Beinker, Nadja M ^a   Lambert, Wietske ^a   Lerch, Mirjam ^a   Daley, Daniel O ^a   Van Wijk, Klaas Jan ^c   Hirst, Judy ^b   Kunji, Edmund ^b   De Gier, Jan Willem ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c Department of Obstetrics Gynecology   (United States)

^d Centre Médical Universitaire   (Switzerland)

Author keywords

Escherichia coli; GFP; Membrane protein characterization; Membrane protein isolation; Membrane protein overexpression

Indexed keywords

GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; PROTEOME;

ARTICLE; ESCHERICHIA COLI; GENE OVEREXPRESSION; MEMBRANE; NONHUMAN; PIPELINE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEOMICS;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GREEN FLUORESCENT PROTEINS; LACTOCOCCUS LACTIS; LUMINESCENT AGENTS; MEMBRANE PROTEINS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 23644458922     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.051466205     Document Type: Article

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