The vitamin-sensitive promoter PTHI11 enables pre-defined autonomous induction of recombinant protein production in Pichia pastoris

Biotechnology and Bioengineering

Volumn 113, Issue 12, 2016, Pages 2633-2643

  Landes, Nils ^a,b   Gasser, Brigitte ^a,b   Vorauer Uhl, Karola ^a   Lhota, Gabriele ^a   Mattanovich, Diethard ^a,b   Maurer, Michael ^b,c  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^c Fachhochschule Campus Wien   (Austria)

Author keywords

Komagataella; Pichia pastoris; recombinant protein; THI11 promoter; thiamine; yeast

Indexed keywords

BATCH CELL CULTURE; BIOCHEMISTRY; CARBON; PROTEINS; YEAST;

CONSTITUTIVE EXPRESSIONS; CONTROLLED ENVIRONMENT; KOMAGATAELLA; PICHIA PASTORIS; RECOMBINANT HUMAN SERUM ALBUMIN; RECOMBINANT PROTEIN PRODUCTIONS; THI11 PROMOTER; THIAMINE;

RECOMBINANT PROTEINS;

CARBON; FUNGAL PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; NITROGEN; PHOSPHORUS; RECOMBINANT HUMAN SERUM ALBUMIN; THIAMINE; RECOMBINANT PROTEIN; REGULATORY RNA SEQUENCE;

ARTICLE; BIOMASS; BIOREACTOR; CONTROLLED STUDY; CULTURE MEDIUM; FUNGAL CELL CULTURE; FUNGAL GENE; FUNGAL STRAIN; FUNGUS GROWTH; GENE EXPRESSION; GROWTH RATE; KOMAGATAELLA PASTORIS; NONHUMAN; PROMOTER REGION; REGULATORY SEQUENCE; THI11 GENE; BIOSYNTHESIS; GENETIC ENHANCEMENT; GENETICS; PHYSIOLOGY; PICHIA; PROCEDURES; PROTEIN ENGINEERING; REGULATORY RNA SEQUENCE;

GENETIC ENHANCEMENT; PICHIA; PROMOTER REGIONS, GENETIC; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; REGULATORY SEQUENCES, RIBONUCLEIC ACID; THIAMINE;

EID: 84978229862     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26041     Document Type: Article

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