Fed-batch operational strategies for recombinant Fab production with Pichia pastoris using the constitutive GAP promoter

Biochemical Engineering Journal

Volumn 79, Issue , 2013, Pages 172-181

  Garcia Ortega, Xavier ^a   Ferrer, Pau ^a   Montesinos, José Luis ^a   Valero, Francisco ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Antibody fragment; Fed batch; PGAP; Pichia pastoris; Recombinant protein production; Specific growth rate; Substrates

Indexed keywords

ANTIBODY FRAGMENT; FED BATCHES; PICHIA PASTORIS; RECOMBINANT PROTEIN PRODUCTIONS; SPECIFIC GROWTH RATE;

AGGREGATES; ANTIBODIES; CARBON; GLUCOSE; GLYCEROL; GROWTH RATE; RECOMBINANT PROTEINS; YEAST;

SUBSTRATES;

GAP JUNCTION PROTEIN; GLYCEROL; IMMUNOGLOBULIN FRAGMENT; OXYGEN; RECOMBINANT ANTIBODY;

ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BIOMASS; BIOPROCESS; CARBON SOURCE; CELL AGGREGATION; CELL GROWTH; FED BATCH CULTURE; GROWTH RATE; HEAT; HUMAN; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; REPRODUCIBILITY;

EID: 84883363291     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2013.07.013     Document Type: Article

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