Recent advances on the GAP promoter derived expression system of Pichia pastoris

Molecular Biology Reports

Volumn 36, Issue 6, 2009, Pages 1611-1619

  Zhang, Ai Lian ^a   Luo, Jin Xian ^b   Zhang, Tian Yuan ^b   Pan, Ying Wen ^a   Tan, Yan Hua ^a   Fu, Ce Yi ^c   Tu, Fa Zhi ^a  

^a INSTITUTE OF TROPICAL BIOSCIENCE AND BIOTECHNOLOGY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

^c Hainan Provincial Institute for Drug Control   (China)

Author keywords

Expression system; GAP promoter; Pichia pastoris

Indexed keywords

ALCOHOL OXIDASE; ANGIOSTATIN; BETA GLUCURONIDASE; EPIDERMAL GROWTH FACTOR; FORMALDEHYDE DEHYDROGENASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; HEPATITIS B SURFACE ANTIGEN; MESSENGER RNA; METHANOL; PROTEIN DISULFIDE ISOMERASE; S ADENOSYLMETHIONINE; SUPEROXIDE DISMUTASE; TUMOR NECROSIS FACTOR;

ARTICLE; CARBON SOURCE; FERMENTATION; GENE EXPRESSION; GENE NUMBER; NONHUMAN; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION;

ANIMALS; CLONING, MOLECULAR; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETIC VECTORS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; PICHIA; PROMOTER REGIONS, GENETIC;

PICHIA PASTORIS;

EID: 67651100721     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-008-9359-4     Document Type: Article

References (58)

1. JM Cregg TS Vedvick WC Raschke 1993 Recent advances in the expression of foreign genes in Pichia pastoris Biotechnology (NY) 11 8 905 910 10.1038/nbt0893-905 (Pubitemid 23218978)
2. P Li A Anumanthan XG Gao K Ilangovan VV Suzara N Düzgü neş V Renugopalakrishnan 2007 Expression of recombinant proteins in Pichia pastoris Appl Biochem Biotechnol 142 105 124 10.1007/s12010-007-0003-x (Pubitemid 47557234)
3. KN Faber W Hander G Ab M Venhuis 1995 Review: methylotrphic yeast as factories for the production of foreign proteins Yeast 11 14 1331 1344 10.1002/yea.320111402
4. S Macauley-Patrick ML Fazenda B McNeil LM Harvey 2005 Heterologous protein production using the Pichia pastoris expression system Yeast 22 249 270 10.1002/yea.1208 (Pubitemid 40528349)
5. SB Ellis PF Brust PJ Koutz AF Waters MM Harpold TR Gingeras 1985 Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris Mol Cell Biol 5 5 1111 1121
6. JF Tschopp PF Brust JM Cregg CA Stilmam TR Gingeras 1987 Expression of the LacZ gene from two methanol-regulated promoters in Pichia pastoris Nucleic Acids Res 15 3859 3876 10.1093/nar/15.9.3859
7. MA Romans 1995 Advances in the use of Pichia pastoris for high level gene expression Curr Opin Biotechnol 6 527 533 10.1016/0958-1669(95)80087-5
8. S Mochizuki N Hamato M Hirose K Miyano W Ohtani S Kameyama S Kuwae T Tokuyama H Ohi 2001 Expression and characterization of recombinant human antithrombin III in Pichia pastoris Protein Expr Purif 23 1 55 65 10.1006/prep.2001.1479 (Pubitemid 32930980)
9. S Shen G Sulter TW Jeffries JM Cregg 1998 A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris Gene 216 1 93 102
10. D Resina O Cos P Ferrer F Valero 2005 Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter Biotechnol Bioeng 91 6 760 767
11. ZA Janowicz MR Eckart C Drewke RO Roggenkamp CP Hollenberg J Maat AM Ledeboer C Visser CT Verrips 1985 Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha Nucleic Acids Res 13 9 3043 3062 10.1093/nar/13.9.3043
12. J Menendez I Valdes N Cabrera 2003 The ICL1 gene of Pichia pastoris, transcriptional regulation and use of its promoter Yeast 20 13 1097 1108
13. IB Sears J O'Connor OW Rossanese BS Glick 1998 A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris Yeast 14 783 790 10.1002/(SICI)1097-0061(19980615)14:<783::AID-YEA272>3.0.CO;2-Y
14. J Ahn J Hong H Lee M Park E Lee C Kim E Choi J Jung H Lee 2007 Translation elongation factor 1-alpha gene from Pichia pastoris: molecular cloning, sequence, and use of its promoter Appl Microbiol Biotechnol 74 3 601 608 10.1007/s00253-006-0698-6 (Pubitemid 46295005)
15. HR Waterham ME Digan PJ Koutz SV Lair JM Cregg 1997 Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter Gene 186 37 44 10.1016/S0378-1119(96)00675-0 (Pubitemid 27084827)
16. G Olezdzka S Dazbrowski J Kur 2003 High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris Protein Expr Purif 29 223 229
17. JM Delroisse M Dannau JJ Gilsoul T El Mejdoub J Destain D Portetelle P Thonart E Haubruge M Vandenbol 2005 Expression of a synthetic gene encoding a Tribolium castaueum carboxylesterase in Pichia pastoris Protein Expr Purif 42 286 294 10.1016/j.pep.2005.04.011 (Pubitemid 40985022)
18. JC Goodrick M Xu R Finnegan BM Schilling S Schiavi H Hoppe NC Wan 2001 High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system Biotechnol Bioeng 74 492 497 10.1002/bit.1140 (Pubitemid 32821753)
19. YP Khasa A Khushoo L Srivastava KJ Mukherjee 2007 Kinetic studies of constitutive human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression in continuous culture of Pichia pastoris Biotechnol Lett 29 12 1903 1908 10.1007/s10529-007-9473-8 (Pubitemid 350043605)
20. Invitrogen Corporation (2002) A manual for expression of recombinant proteins using pGAPZ and pGAPZαin Pichia pastoris. Invitrogen Corporation, San Diego, CA
21. AL Zhang JX Luo TY Zhang SC Chen WJ Guan 2004 Constitutive expression of human angiostatin in Pichia pastoris using the GAP promoter Acta Genetica Sin 31 6 552 557
22. GH Chen LJ Yin IH Chiang ST Jiang 2007 Expression and purification of goat lactoferrin from Pichia pastoris expression system J Food Sci 72 67 71 10.1111/j.1750-3841.2007.00281.x (Pubitemid 46392393)
23. P Rupa S Nakamura Y Mine 2007 Genetically glycosylated ovomucoid third domain can modulate immunoglobulin E antibody production and cytokine response in BALB/c mice Clin Exp Allergy 37 918 928 10.1111/j.1365-2222.2007.02720.x (Pubitemid 47074552)
24. ZL Yu XJ Wu DY Li S Yang Z Zhou J Cai ZY Yuan 2003 Enhancement of the production of SAM by overexpression of SAM synthetase in Pichia pastoris Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35 127 132
25. F Döring M Klapper S Theis H Daniel 1998 Use of the glyceraldehyde-3-phosphate dehydrogenase promoter for production of functional mammalian membrane transport proteins in the yeast Pichia pastoris Biochem Biophys Res Commun 250 531 535 10.1006/bbrc.1998.9342 (Pubitemid 28463913)
26. A Vassileva DA Chugh S Swaminathan N Khanna 2001 Expression of hepatitis B surface antigen in the methytotropic yeast P. pastoris using the GAP promoter J Biotechnol 88 21 35 10.1016/S0168-1656(01)00254-1 (Pubitemid 32480115)
27. H Boer TT Teeri A Koivula 2000 Characterization of Trichoderma reesei cellobiohydrolase Ce17A secreted from Pichia pastoris using two different promoters Biotechnol Bioeng 69 486 494 10.1002/1097-0290(20000905)69:5<486:: AID-BIT3>3.0.CO;2-N
28. RJ Baerends GJ Sulter TW Jeffries JM Cregg M Veenhuis 2002 Molecular characterization of the Hansenula polymorpha FLD1 gene encoding formaldehyde dehydrogenase Yeast 19 1 37 42 10.1002/yea.805 (Pubitemid 34083050)
29. JL Cereghino JM Cregg 2000 Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiol Rev 24 1 45 66 10.1111/j.1574-6976.2000.tb00532.x
30. N Segev J Mulholland D Botstein 1998 The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery Cell 52 915 924 10.1016/0092-8674(88)90433-3
31. JG Zhang XD Wang JN Zhang DZ Wei 2008 Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source J Biosci Bioeng 105 4 335 340 10.1263/jbb.105.335 (Pubitemid 351680730)
32. JL Xie L Zhang Q Ye QW Zhou L Xin P Du RB Gan 2003 Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources Biotechnol Lett 25 173 177 10.1023/A:1021905010021 (Pubitemid 36189365)
33. R Ramón P Ferrer F Valero 2007 Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris J Biotechnol 130 1 39 46 10.1016/j.jbiotec.2007.02.025 (Pubitemid 46634651)
34. C Jungo J Schenk M Pasquier IW Marison U von Stockar 2007 A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris J Biotechnol 131 1 57 66 10.1016/j.jbiotec.2007.05.019 (Pubitemid 47042930)
35. AL Zhang TY Zhang JX Luo SC Chen WJ Guan CY Fu SQ Peng HL Li 2007 Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture J Ind Microbiol Biotechnol 34 117 122 10.1007/s10295-006-0175-3 (Pubitemid 46147392)
36. O Cos R Ramón JL Montesinos F Valero 2006 Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris: a review Microb Cell Fact 5 17 10.1186/1475-2859-5-17 (Pubitemid 44424222)
37. H Hohenblum B Gasser M Maurer N Borth D Mattanovich 2004 Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris Biotechnol Bioeng 85 367 375 10.1002/bit.10904 (Pubitemid 38186091)
38. CA Scorer JJ Clare WR McCombie MA Romanos K Sreekrishna 1994 Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression Biotechnology (NY) 12 2 181 184 10.1038/nbt0294-181 (Pubitemid 24045353)
39. M Inan SA Fanders W Zhang PJ Hotez B Zhan MM Meagher 2007 Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1) Methods Mol Biol 389 65 76 10.1007/978-1-59745-456-8-5
40. H Zhu J Shi JM Cregg G Woldegiorgis 1997 Reconstitution of highly expressed human heart muscle carnitine palmitoyltransferase I Biochem Biophys Res Commun 239 2 498 502 10.1006/bbrc.1997.7494 (Pubitemid 27488993)
41. JJ Clare FB Rayment SP Ballantine K Sreekrishna MA Romanos 1991 High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene Biotechnology (NY) 9 5 455 460 10.1038/nbt0591-455
42. R Daly MT Hearn 2005 Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production J Mol Recognit 18 2 119 138 10.1002/jmr.687 (Pubitemid 40328574)
43. M Mansur C Cabello L Hernández J País L Varas J Valdés Y Terrero A Hidalgo L Plana V Besada L García E Lamazares L Castellanos E Martínez 2005 Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris Biotechnol Lett 27 5 339 345 10.1007/s10529-005-1007-7 (Pubitemid 40564458)
44. HY Luo HQ Huang YG Bai YR Wang PL Yang K Meng TZ Yuan B Yao 2006 Improving phytase expression by increasing the gene copy number of appA-m in Pichia pastoris Sheng Wu Gong Cheng Xue Bao 22 4 528 533
45. HL Chen CC Yen TC Tsai CH Yu YJ Liou YW Lai ML Wang CM Chen 2006 Production and characterization of human extracellular superoxide dismutase in the methylotrophic yeast Pichia pastoris J Agric Food Chem 54 8041 8047 10.1021/jf061379x (Pubitemid 44785382)
46. JM Zhou YX Tang DY Fang JJ Zhou Y Liang HY Guo LF Jiang 2006 Secreted expression and purification of dengue 2 virus full-length nonstructural glycoprotein NS1 in Pichia pastoris Virus Genes 33 1 27 32 10.1007/s11262-005- 0036-6 (Pubitemid 43964701)
47. TY Zhang JX Luo XY Lu 2002 Cloning and expression of kringle 1-3 gene of human plasminogen and the purification and bioactivity of its expressed product Sheng Wu Gong Cheng Xue Bao 18 5 593 596
48. JL Ding EH Lim HF Li JK Kumar SL Lee TJ Lam 2004 Expression of recombinant vitellogenin in the yeast Pichia pastoris Biotechnol Bioeng 85 330 339 10.1002/bit.10800 (Pubitemid 38182226)
49. ST Jiang GH Chen SJ Tang CS Chen 2002 Effect of glycosylation modification (N-Q-(108)I - > N-Q-(108)T) on the freezing stability of recombinant chicken Cystatin overexpressed in Pichia pastoris X-33 J Agric Food Chem 50 5313 5317 10.1021/jf0200321 (Pubitemid 35001287)
50. SH Wang TS Yang SM Lin MS Tsai SC Wu SJ Mao 2002 Expression, characterization, and purification of recombinant porcine lactoferrin in Pichia pastoris Protein Expr Purif 25 1 41 49 10.1006/prep.2001.1607 (Pubitemid 36034999)
51. SH Liu WI Chou CC Sheu MD Chang 2005 Improved secretory production of glucoamylase in Pichia pastoris by combination of genetic manipulations Biochem Biophys Res Commun 326 4 817 824 10.1016/j.bbrc.2004.11.112 (Pubitemid 39655993)
52. JD Chung 2000 Design of metabolic feed controllers: applications to high-density fermentations of Pichia pastoris Biotechnol Bioeng 68 298 307 10.1002/(SICI)1097-0290(20000505)68:3<298::AID-BIT8>3.0.CO;2-K
53. K Sreekrishna RG Brankamp KE Kropp DT Blankenship JT Tsay PL Smith JD Wierschke A Subramaniam LA Birkenberger 1997 Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris Gene 190 55 62 10.1016/S0378-1119(96)00672-5 (Pubitemid 27214270)
54. Sreekrishna K, Kropp KE (1996) Pichia pastoris. In: Wolf K (ed) Non-conventional yeast in biotechnology: a handbook. Springer, Berlin, pp 203-252
55. J Lin D Panigraphy LB Trinh J Folkman J Shiloach 2000 Production process for recombinant human angiostatin in Pichia pastoris J Ind Microbiol Biotechnol 24 31 35 10.1038/sj.jim.2900766
56. W Zhao J Wang R Deng X Wang 2008 Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter J Ind Microbiol Biotechnol 35 3 189 195 10.1007/s10295-007-0283-8
57. JM Wu JC Lin LL Chieng CK Lee TA Hsu 2003 Combined use of GAP and AOXl promoter to enhance the expression of human granulocyte-macrophage colony-stimulating factor in Pichia pastoris Enzym Microb Technol 33 453 459 10.1016/S0141-0229(03)00147-9 (Pubitemid 37011395)
58. JM Wu LL Chieng TA Hsu CK Lee 2003 Sequential expression of recombinant proteins and their separate recovery from a Pichia pastoris cultivation Biochem Eng J 16 9 16 10.1016/S1369-703X(03)00017-2 (Pubitemid 37064215)