Repressible promoters - A novel tool to generate conditional mutants in Pichia pastoris

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Delic, Marizela ^a,b   Mattanovich, Diethard ^a,b   Gasser, Brigitte ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

Pichia pastoris; Promoter exchange; Repressible promoter

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; FUNGAL RNA;

ARTICLE; DOWN REGULATION; ERO1 GENE; FUNGUS CULTURE; FUNGUS MUTANT; GENE; GENE AMPLIFICATION; GENE EXPRESSION REGULATION; GENE REPRESSION; MOLECULAR CLONING; NONHUMAN; PDI1 GENE; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REPRESSIBLE PROMOTER; RNA ISOLATION;

DOWN-REGULATION; GREEN FLUORESCENT PROTEINS; MUTATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

PICHIA PASTORIS;

EID: 84872686644     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-6     Document Type: Article

References (16)

1. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22:249-270. 10.1002/yea.1208, 15704221.
2. Damasceno LM, Huang CJ, Batt CA. Protein secretion in Pichia pastoris and advances in protein production. Appl Microbiol Biotechnol 2012, 93:31-39. 10.1007/s00253-011-3654-z, 22057543.
3. Delic M, Rebnegger C, Wanka F, Puxbaum V, Haberhauer-Troyer C, Hann S, Kollensperger G, Mattanovich D, Gasser B. Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast. Free Radic Biol Med 2012, 52:2000-2012. 10.1016/j.freeradbiomed.2012.02.048, 22406321.
4. Till A, Lakhani R, Burnett SF, Subramani S. Pexophagy: the selective degradation of peroxisomes. Int J Cell Biol 2012, 2012:512721. 3320016, 22536249.
5. Sakai Y, Oku M, van der Klei IJ, Kiel JA. Pexophagy: autophagic degradation of peroxisomes. Biochim Biophys Acta 2006, 1763:1767-1775. 10.1016/j.bbamcr.2006.08.023, 17005271.
6. Maya D, Quintero MJ, de la Cruz Muñoz-Centeno M, Chávez S. Systems for applied gene control in Saccharomyces cerevisiae. Biotechnol Lett 2008, 30:979-987. 10.1007/s10529-008-9647-z, 18239858.
7. Tschopp JF, Brust PF, Cregg JM, Stillman CA, Gingeras TR. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 1987, 15:3859-3876. 10.1093/nar/15.9.3859, 340787, 3108861.
8. Stadlmayr G, Mecklenbräuker A, Rothmüller M, Maurer M, Sauer M, Mattanovich D, Gasser B. Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production. J Biotechnol 2010, 150:519-529.
9. Anderson MS, Lopes JM. Carbon source regulation of PIS1 gene expression in Saccharomyces cerevisiae involves the MCM1 gene and the two-component regulatory gene, SLN1. J Biol Chem 1996, 271:26596-26601. 10.1074/jbc.271.43.26596, 8900132.
10. Han SH, Han GS, Iwanyshyn WM, Carman GM. Regulation of the PIS1-encoded phosphatidylinositol synthase in Saccharomyces cerevisiae by zinc. J Biol Chem 2005, 280:29017-29024. 10.1074/jbc.M505881200, 1201514, 15980062.
11. Mao X, Hu Y, Liang C, Lu C. MET3 promoter: a tightly regulated promoter and its application in construction of conditional lethal strain. Curr Microbiol 2002, 45:37-40. 10.1007/s00284-001-0046-0, 12029525.
12. Dünkler A, Wendland J. Use of MET3 promoters for regulated gene expression in Ashbya gossypii. Curr Genet 2007, 52:1-10. 10.1007/s00294-007-0134-1, 17479268.
13. Jani NM, Lopes JM. Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p. Mol Microbiol 2008, 70:1529-1539. 10.1111/j.1365-2958.2008.06506.x, 19019152.
14. Giaever G, Chu AM, Ni L, Connelly C, Riles L, Véronneau S, Dow S, Lucau-Danila A, Anderson K, André B. Functional profiling of the Saccharomyces cerevisiae genome. Nature 2002, 418:387-391. 10.1038/nature00935, 12140549.
15. Marx H, Mattanovich D, Sauer M. Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris. Microb Cell Fact 2008, 7:23. 10.1186/1475-2859-7-23, 2517057, 18664246.
16. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001, 29:e45. 10.1093/nar/29.9.e45, 55695, 11328886.