Using Schizosaccharomyces pombe as a host for expression and purification of eukaryotic proteins

Gene

Volumn 200, Issue 1-2, 1997, Pages 135-144

  Lu, Quinn ^a   Bauer, John C ^a   Greener, Alan ^a  

^a Stratagene   (United States)

Author keywords

FLAG epitope vectors; Glutathione S transferase; nmt1 promoter; Protein expression and purification; S. pombe

Indexed keywords

AGAROSE; ENTEROPEPTIDASE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; THROMBIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE INDUCTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; SCHIZOSACCHAROMYCES POMBE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CLONING, MOLECULAR; ESCHERICHIA COLI; FUNGAL PROTEINS; GENES, HELMINTH; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; HUMANS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MAP KINASE KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; PEPTIDES; PROMOTER REGIONS (GENETICS); PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SCHISTOSOMA JAPONICUM; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE TAGGED SITES;

EID: 0030728354     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00393-4     Document Type: Article

References (23)

1. Aslanidis, C., de Jong, P.J., 1990. Ligation-independent cloning of PCR product (LIC-PCR). Nucleic Acids Res. 18, 6069-6074.
2. Chinami, M., Shingu, M., 1992. Construction of an expresson vector for human papillomavirus type 16 E7-glutathione S-transferase fusion protein. Kurume Med. J. (Japan) 39, 9-12.
3. Cottarel, G., The Saccharomyces cerevisiae HIS3 and LYS2 genes complement the Schizosaccharomycespombe his5-303 and lys1-131 mutations, respectively: new selectable markers and new multi-purpose multicopy shuttle vectors, pSP3 and pSP4. 1995. Curr. Genet. 28, 380-383.
4. Crews, C.M., Alessandrini, A., Erikson, R.L., 1992. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258, 478-480.
5. Forsburg, S., 1993. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21, 2955-2956.
6. Ghosh, S., Basu, S., Strum, J.C., Basu, S., Bell, R.M., 1995. Identification of conditions that facilitate the expression of GST fusions as soluble, full-length proteins. Anal. Biochem. 225, 376-378.
7. Habig, W.H., Pabst, M.J., Jakoby, W.B., 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139.
8. Hopp, H.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P., Urdal, D.L., Conlon, P.J., 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6, 1204-1210.
9. Huan, R., Moss, J., 1992. Ligation-independent cloning of glutathione S-transferase fusion genes for expression in Escherichia coli. Gene 112, 37-43.
10. Huan, R.S., Serventi, I.M., Moss, J., 1992. Rapid reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13, 515-518.
11. La Vallie, E.R., Rehemtulla, A., Racie, L.A., DiBlasio, E.A., Ferenz, C., Grank, K.L., Light, A., McCoy, J.M., 1993. Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase. J. Biol. Chem. 268, 23311-23317.
12. Lee, M.G., Nurse, P., 1987. Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature 327, 31-35.
13. Maundrell, K., 1989. Nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. J. Biol. Chem. 265, 10857-10864.
14. Maundrell, K., 1993. Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123, 127-130.
15. Moreno, S., Klar, A., Nurse, P., 1991. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823.
16. Romanos, M.A., Scorer, C.A., Clare, J.J., 1992. Foreign gene expression in yeast: a review. Yeast 8, 423-488.
17. Romanos, M.A., Hughes, F.J., Comerford, S.A., Scorer, C.A., 1995. Production of a phosphorylated GST::HPV-6 E7 fusion protein using a yeast expression vector and glutathione S-transferase fusions. Gene 152, 137-138.
18. Sambrook, J., Fritsch, E. F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Schneider, J.C., Guarente, L., 1989. Vectors for expression of cloned genes in yeast: regulation, overproduction, and underproduction. Methods Enzymol. 194, 373-388.
20. Sipiczki, M., 1989. Taxonomy and phylogenesis. In: Nisim, A., Young, P., Johnson, B.F. (Eds.), Molecular Biology of the Fission Yeast. Academic Press, San Diego, pp. 431-452.
21. Smith, D.B., Johnson, K.S., 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
22. Tommasino, M., Contorni, M., Cavalieri, F., 1992. HPV16 E7 phosphorylation in fission yeast: characterization and biological effects. Gene 111, 93-98.
23. Zheng, C.-F., Guan, K.-L., 1993. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinase, MEK1 and MEK2. J. Biol. Chem. 268, 11435-11439.