Induction without methanol: Novel regulated promoters enable high-level expression in Pichia pastoris

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Prielhofer, Roland ^a   Maurer, Michael ^b,c   Klein, Joachim ^d   Wenger, Jana ^d   Kiziak, Christoph ^d   Gasser, Brigitte ^a,b   Mattanovich, Diethard ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^c Fachhochschule Campus Wien   (Austria)

^d LONZA AG   (Switzerland)

Author keywords

Glucose limited fed batch cultivation; Heterologous protein production; High affinity glucose transporter; Inducible promoter; Pichia pastoris

Indexed keywords

CARBON; CARBOXYPEPTIDASE B; ENHANCED GREEN FLUORESCENT PROTEIN; GLUCOSE; GLUCOSE TRANSPORTER; GLUCOSE TRANSPOTER H1; GLYCEROL; HUMAN SERUM ALBUMIN; IMMUNOGLOBULIN F(AB) FRAGMENT; METHANOL; UNCLASSIFIED DRUG;

ANTIBODY TITER; ARTICLE; BATCH FERMENTATION; BINDING AFFINITY; BIOMASS DISTRIBUTION; CELL GROWTH; CONTROLLED STUDY; DNA MICROARRAY; FED BATCH CULTURE; FEEDING; GENE AMPLIFICATION; GENE DOSAGE; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE IDENTIFICATION; GENE INDUCTION; GENE REPRESSION; GENE VECTOR; HETEROLOGOUS EXPRESSION; LARGE SCALE PRODUCTION; MOLECULAR GENETICS; PICHIA PASTORIS; PROMOTER REGION; PROTEIN SECRETION; WILD TYPE;

ANIMALS; BATCH CELL CULTURE TECHNIQUES; BIOMASS; BIOREACTORS; CARBOXYPEPTIDASE B; GENE DOSAGE; GLUCOSE; GREEN FLUORESCENT PROTEINS; HUMANS; METHANOL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; SERUM ALBUMIN; SWINE;

PICHIA PASTORIS; SUS;

EID: 84872687906     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-5     Document Type: Article

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