Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

Nature Protocols

Volumn 10, Issue 11, 2015, Pages 1860-1896

  Treweek, Jennifer B ^a   Chan, Ken Y ^a   Flytzanis, Nicholas C ^a   Yang, Bin ^a   Deverman, Benjamin E ^a   Greenbaum, Alon ^a   Lignell, Antti ^b   Xiao, Cheng ^a   Cai, Long ^b   Ladinsky, Mark S ^a   Bjorkman, Pamela J ^a   Fowlkes, Charless C ^c   Gradinaru, Viviana ^a  

^a California Institute of Technology   (United States)

^b California Institute of Technology   (United States)

^c Irvine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ANIMAL TISSUE; ARTICLE; CLINICAL PROTOCOL; CONCENTRATION (PARAMETERS); CONFOCAL MICROSCOPY; EMBEDDING; EXPERIMENTAL DESIGN; EXPERIMENTAL STUDY; EXTRACTION; FEMALE; HISTOLOGY; HUMAN; HUMAN TISSUE; HYDROGEL; INCUBATION TIME; MALE; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAT; THICKNESS; THREE DIMENSIONAL IMAGING; TISSUE FIXATION; ANIMAL; CYTOCHEMISTRY; FLUORESCENCE IMAGING; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; PATHOLOGY; PROCEDURES; STAINING; TIME;

DETERGENT; LIPID;

ANIMALS; DETERGENTS; HISTOCYTOCHEMISTRY; LIPIDS; MICE; OPTICAL IMAGING; PATHOLOGY; RATS; SPECIMEN HANDLING; STAINING AND LABELING; TIME FACTORS; TISSUE EMBEDDING; TISSUE FIXATION;

EID: 84965191403     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2015.122     Document Type: Article

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