Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

Nature

Volumn 450, Issue 7166, 2007, Pages 56-62

  Livet, Jean ^a   Weissman, Tamily A ^a   Kang, Hyuno ^a   Draft, Ryan W ^a   Lu, Ju ^a   Bennis, Robyn A ^a   Sanes, Joshua R ^a   Lichtman, Jeff W ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; FLUORESCENT PROTEIN; PROTEIN DERIVATIVE; UNCLASSIFIED DRUG;

GENE EXPRESSION; GENETIC ENGINEERING; NERVOUS SYSTEM; PROTEIN; RECOMBINATION; RODENT;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL INTERACTION; CELL LABELING; CEREBELLUM; CONTROLLED STUDY; GENETIC PROCEDURES; GLIA CELL; HUMAN; HUMAN CELL; MOUSE; NERVE CELL NETWORK; NERVE FIBER; NERVOUS SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SYNAPSE; TRANSGENE; TRANSGENIC MOUSE; TRANSGENICS;

MUS MUSCULUS;

EID: 35948992641     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature06293     Document Type: Article

References (42)

1. Denk, W. & Horstmann, H. Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol. 2, e329 (2004).
2. Briggman, K. L. & Denk, W. Towards neural circuit reconstruction with volume electron microscopy techniques. Curr. Opin. Neurobiol. 16, 562-570 (2006).
3. Micheva, K. D. & Smith, S. J. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron 55, 25-36 (2007).
4. Gan, W. B., Grutzendler, J., Wong, W. T., Wong, R. O.& Lichtman, J. W. Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron 27, 219-225 (2000).
5. Shaner, N. C., Steinbach, P. A. & Tsien, R. Y. A guide to choosing fluorescent proteins. Nature Methods 2, 905-909 (2005).
6. Hadjantonakis, A. K., Macmaster, S. & Nagy, A. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol. 2, 11 (2002).
7. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51 (2000).
8. Kasthuri, N. & Lichtman, J. W. The role of neuronal identity in synaptic competition. Nature 424, 426-430 (2003).
9. Zong, H., Espinosa, J. S., Su, H. H., Muzumdar, M. D. & Luo, L. Mosaic analysis with double markers in mice. Cell 121, 479-492 (2005).
10. Branda, C. S. & Dymecki, S. M. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice. Dev. Cell 6, 7-28 (2004).
11. Lam, K. P. & Rajewsky, K. Rapid elimination of mature autoreactive B cells demonstrated by Cre-induced change in B cell antigen receptor specificity in vivo. Proc. Natl Acad. Sci. USA 95, 13171-13175 (1998).
12. Lee, G. & Saito, I. Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination. Gene 216, 55-65 (1998).
13. Caroni, P. Overexpression of growth-associated proteins in the neurons of adult transgenic mice. J. Neurosci. Methods 71, 3-9 (1997).
14. Guo, C., Yang, W. & Lobe, C. G. A Cre recombinase transgene with mosaic, widespread tamoxifen-inducible action. Genesis 32, 8-18 (2002).
15. Metzger, D., Clifford, J., Chiba, H. & Chambon, P. Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase. Proc. Natl Acad. Sci. USA 92, 6991-6995 (1995).
16. Garrick, D., Fiering, S., Martin, D. I. & Whitelaw, E. Repeat-induced gene silencing in mammals. Nature Genet. 18, 56-59 (1998).
17. Palmiter, R. D. &Brinster, R. L. Germ-line transformation of mice. Annu. Rev. Genet. 20, 465-499 (1986).
18. Rodriguez, C. I. & Dymecki, S. M. Origin of the precerebellar system. Neuron 27, 475-486 (2000).
19. Rowan, S. & Cepko, C. L. Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter. Dev. Biol. 271, 388-402 (2004).
20. Eccles, J. C., Ito, M. & Szentágothai, J. in The Cerebellum as a Neuronal Machine (Springer, Berlin, 1967).
21. Wu, H. S., Sugihara, I. & Shinoda, Y. Projection patterns of single mossy fibers originating from the lateral reticular nucleus in the rat cerebellar cortex and nuclei. J. Comp. Neurol. 411, 97-118 (1999).
22. Palay, S. L. & Chan-Palay, V. in Cerebellar Cortex (Springer, New York, 1974).
23. Bushong, E. A., Martone, M. E., Jones, Y. Z. & Ellisman, M. H. Protoplasmic astrocytes in CA1 stratum radiatum occupy separate anatomical domains. J. Neurosci. 22, 183-192 (2002).
24. Ogata, K. & Kosaka, T. Structural and quantitative analysis of astrocytes in the mouse hippocampus. Neuroscience 113, 221-233 (2002).
25. Silver, D. P. & Livingston, D. M. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol. Cell 8, 233-243 (2001).
26. Gong, S. et al. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425, 917-925 (2003).
27. Lein, E. S. et al. Genome-wide atlas of gene expression in the adult mouse brain. Nature 445, 168-176 (2007).
28. Zacharias, D. A., Violin, J. D., Newton, A. C. & Tsien, R. Y. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296, 913-916 (2002).
29. Rizzo, M. A., Springer, G. H., Granada, B. & Piston, D. W. An improved cyan fluorescent protein variant useful for FRET. Nature Biotechnol. 22, 445-449 (2004).
30. Karasawa, S., Araki, T., Nagai, T., Mizuno, H. & Miyawaki, A. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381, 307-312 (2004).
31. Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl Acad. Sci. USA 99, 7877-7882 (2002).
32. Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnol. 22, 1567-1572 (2004).
33. Kay, J. N. et al. Transient requirement for ganglion cells during assembly of retinal synaptic layers. Development 131, 1331-1342 (2004).
34. Buffelli, M. et al. Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition. Nature 424, 430-434 (2003).
35. Zuo, Y. et al. Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination. J. Neurosci. 24, 10999-11009 (2004).
36. Fiala, J. C. Reconstruct: a free editor for serial section microscopy. J. Microsc. 218, 52-61 (2005).
37. Kakoki, M. et al. Altering the expression in mice of genes by modifying their 3′ regions. Dev. Cell 6, 597-606 (2004).
38. Srinivas, S. et al. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev. Biol. 1, 4 (2001).
39. Marquardt, T. et al. Pax6 is required for the multipotent state of retinal progenitor cells. Cell 105, 43-55 (2001).
40. Gorski, J. A. et al. Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J. Neurosci. 22, 6309-6314 (2002).
41. Ringrose, L., Chabanis, S., Angrand, P. O., Woodroofe, C. & Stewart, A. F. Quantitative comparison of DNA looping in vitro and in vivo: chromatin increases effective DNA flexibility at short distances. EMBO J. 18, 6630-6641 (1999).
42. Zheng, B., Sage, M., Sheppeard, E. A., Jurecic, V. & Bradley, A. Engineering mouse chromosomes with Cre-loxP: range, efficiency, and somatic applications. Mol. Cell. Biol. 20, 648-655 (2000).