A rapid optical clearing protocol using 2,2′-thiodiethanol for microscopic observation of fixed mouse brain

PLoS ONE

Volumn 10, Issue 1, 2015, Pages

  Aoyagi, Yuka ^a   Kawakami, Ryosuke ^a,b   Osanai, Hisayuki ^a   Hibi, Terumasa ^a,b   Nemoto, Tomomi ^a,b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

2,2' THIODIETHANOL; ALCOHOL; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN; 2,2'-THIODIETHANOL; THIOL DERIVATIVE;

ADULT; ANALYTIC METHOD; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN NERVE CELL; CONCENTRATION (PARAMETERS); CONFOCAL MICROSCOPY; CONTROLLED STUDY; DENDRITIC SPINE; IMMERSION; MICROSCOPY; MOUSE; NONHUMAN; PROTEIN EXPRESSION; PYRAMIDAL NERVE CELL; TWO PHOTON MICROSCOPY; ANIMAL; BRAIN; DENDRITE; NERVE CELL; ULTRASTRUCTURE;

ANIMALS; BRAIN; DENDRITES; DENDRITIC SPINES; MICE; MICROSCOPY, CONFOCAL; NEURONS; SULFHYDRYL COMPOUNDS;

EID: 84961288329     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0116280     Document Type: Article

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