Transgene expression and effective gene silencing in vagal afferent neurons in vivo using recombinant adeno-associated virus vectors

Journal of Physiology

Volumn 588, Issue 21, 2010, Pages 4303-4315

  Kollarik, M ^a   Carr, M J ^b   Ru, F ^a   Ring, C J A ^c   Hart, V J ^c   Murdock, P ^c   Myers, A C ^a   Muroi, Y ^a   Undem, B J ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b GLAXOSMITHKLINE   (United States)

^c GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; ENHANCED GREEN FLUORESCENT PROTEIN; SHORT HAIRPIN RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL TRANSPORT; CONTROLLED STUDY; GENE EXPRESSION; GENE SILENCING; IN VIVO STUDY; MORPHOLOGY; NERVE ENDING; NERVE FUNCTION; NONHUMAN; PARASYMPATHETIC NERVE; PRIORITY JOURNAL; SENSORY GANGLION; SENSORY NERVE; SIGNAL TRANSDUCTION; SPINAL GANGLION; TRANSGENE;

ADENOVIRIDAE; ANIMALS; ANIMALS, GENETICALLY MODIFIED; GENE SILENCING; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; GUINEA PIGS; MODELS, ANIMAL; NEURONS, AFFERENT; NODOSE GANGLION; PATCH-CLAMP TECHNIQUES; TRPV CATION CHANNELS;

EID: 78049466487     PISSN: 00223751     EISSN: 14697793     Source Type: Journal    
DOI: 10.1113/jphysiol.2010.192971     Document Type: Article

References (33)

1. Adriaensen D, Brouns I, Pintelon I, De Proost I & Timmermans JP (2006). Evidence for a role of neuroepithelial bodies as complex airway sensors: comparison with smooth muscle-associated airway receptors. J Appl Physiol 101, 960-970.
2. Agostoni E, Chinnock JE, De Daly MB & Murray JG (1957). Functional and histological studies of the vagus nerve and its branches to the heart, lungs and abdominal viscera in the cat. J Physiol 135, 182-205.
3. Asala SA & Bower AJ (1986). An electron microscope study of vagus nerve composition in the ferret. Anat Embryol (Berl) 175, 247-253.
4. Canning BJ, Mazzone SB, Meeker SN, Mori N, Reynolds SM & Undem BJ (2004). Identification of the tracheal and laryngeal afferent neurones mediating cough in anaesthetized guinea-pigs. J Physiol 557, 543-558.
5. Castro M, Griffiths D, Patel A, Pattrick N, Kitson C & Ladlow M (2004). Effect of chain length on transfection properties of spermine-based gemini surfactants. Org Biomol Chem 2, 2814-2820.
6. Cummins TR, Rush AM, Estacion M, Dib-Hajj SD & Waxman SG (2009). Voltage-clamp and current-clamp recordings from mammalian DRG neurons. Nat Protoc 4, 1103-1112.
7. Ehlert EM, Eggers R, Niclou SP & Verhaagen J (2010). Cellular toxicity following application of adeno-associated viral vector-mediated RNA interference in the nervous system. BMC Neurosci 11, 20.
8. Evans CH, Cooney AM & DiPaolo JA (1975). Colony inhibition mediated by nonimmune leukocytes in vitro and skin reactivity in vivo as indices of tumorigenicity of guinea pig cultures transformed by chemical carcinogens. Cancer Res 35, 1045-1052.
9. Gabella G & Pease HL (1973). Number of axons in the abdominal vagus of the rat. Brain Res 58, 465-469.
10. Gao GP, Alvira MR, Wang L, Calcedo R, Johnston J & Wilson JM (2002). Novel adeno-associated viruses from rhesus monkeys as vectors for human gene therapy. Proc Natl Acad Sci U S A 99, 11854-11859.
11. Glatzel M, Flechsig E, Navarro B, Klein MA, Paterna JC, Bueler H & Aguzzi A (2000). Adenoviral and adeno-associated viral transfer of genes to the peripheral nervous system. Proc Natl Acad Sci U S A 97, 442-447.
12. Kollarik M, Ru F & Brozmanova M (2010). Vagal afferent nerves with the properties of nociceptors. Auton Neurosci 153, 12-20.
13. Kummer W, Fischer A, Kurkowski R & Heym C (1992). The sensory and sympathetic innervation of guinea-pig lung and trachea as studied by retrograde neuronal tracing and double-labelling immunohistochemistry. Neuroscience 49, 715-737.
14. Kwong K, Kollarik M, Nassenstein C, Ru F & Undem BJ (2008). P2×2 receptors differentiate placodal vs. neural crest C-fiber phenotypes innervating guinea pig lungs and esophagus. Am J Physiol Lung Cell Mol Physiol 295, L858-L865.
15. Lee MG, Macglashan DW Jr & Undem BJ (2005). Role of chloride channels in bradykinin-induced guinea pig airway vagal C-fibre activation. J Physiol 566, 205-212.
16. 2+ changes in human lung mast cells: graded vs. all-or-nothing elevations after IgE-mediated stimulation. J Cell Biol 109, 123-134.
17. Mason MR, Ehlert EM, Eggers R, Pool CW, Hermening S, Huseinovic A, Timmermans E, Blits B & Verhaagen J (2010). Comparison of AAV serotypes for gene delivery to dorsal root ganglion neurons. Mol Ther 18, 715-724.
18. Mazzone SB & McGovern AE (2008). Immunohistochemical characterization of nodose cough receptor neurons projecting to the trachea of guinea pigs. Cough 4, 9.
19. Mazzone SB, Reynolds SM, Mori N, Kollarik M, Farmer DG, Myers AC & Canning BJ (2009). Selective expression of a sodium pump isozyme by cough receptors and evidence for its essential role in regulating cough. J Neurosci 29, 13662-13671.
20. Ni D & Lee LY (2008). Effect of increasing temperature on TRPV1-mediated responses in isolated rat pulmonary sensory neurons. Am J Physiol Lung Cell Mol Physiol 294, L563-L571.
21. Riccio MM, Kummer W, Biglari B, Myers AC & Undem BJ (1996). Interganglionic segregation of distinct vagal afferent fibre phenotypes in guinea-pig airways. J Physiol 496, 521-530.
22. Sant'Ambrogio G & Widdicombe J (2001). Reflexes from airway rapidly adapting receptors. Respir Physiol 125, 33-45.
23. Storek B, Reinhardt M, Wang C, Janssen WG, Harder NM, Banck MS, Morrison JH & Beutler AS (2008). Sensory neuron targeting by self-complementary AAV8 via lumbar puncture for chronic pain. Proc Natl Acad Sci U S A 105, 1055-1060.
24. Taylor-Clark TE, Kollarik M, MacGlashan DW Jr & Undem BJ (2005). Nasal sensory nerve populations responding to histamine and capsaicin. J Allergy Clin Immunol 116, 1282-1288.
25. Towne C, Pertin M, Beggah AT, Aebischer P & Decosterd I (2009). Recombinant adeno-associated virus serotype 6 (rAAV2/6)-mediated gene transfer to nociceptive neurons through different routes of delivery. Mol Pain 5, 52.
26. Undem BJ, Chuaychoo B, Lee MG, Weinreich D, Myers AC & Kollarik M (2004). Subtypes of vagal afferent C-fibres in guinea-pig lungs. J Physiol 556, 905-917.
27. Wank M & Neuhuber WL (2001). Local differences in vagal afferent innervation of the rat esophagus are reflected by neurochemical differences at the level of the sensory ganglia and by different brainstem projections. J Comp Neurol 435, 41-59.
28. Xu Y, Gu Y, Wu P, Li GW & Huang LY (2003a). Efficiencies of transgene expression in nociceptive neurons through different routes of delivery of adeno-associated viral vectors. Hum Gene Ther 14, 897-906.
29. Xu Y, Gu Y, Xu GY, Wu P, Li GW & Huang LY (2003b). Adeno-associated viral transfer of opioid receptor gene to primary sensory neurons: a strategy to increase opioid antinociception. Proc Natl Acad Sci U S A 100, 6204-6209.
30. Yu S, Ru F, Ouyang A & Kollarik M (2008). 5-Hydroxytryptamine selectively activates the vagal nodose C-fibre subtype in the guinea-pig oesophagus. Neurogastroenterol Motil 20, 1042-1050.
31. Yu S, Undem BJ & Kollarik M (2005). Vagal afferent nerves with nociceptive properties in guinea-pig oesophagus. J Physiol 563, 831-842.
32. Zagorodnyuk VP & Brookes SJ (2000). Transduction sites of vagal mechanoreceptors in the guinea pig esophagus. J Neurosci 20, 6249-6255.
33. Zagorodnyuk VP, Chen BN, Costa M & Brookes SJ (2003). Mechanotransduction by intraganglionic laminar endings of vagal tension receptors in the guinea-pig oesophagus. J Physiol 553, 575-587.