A multispectral LED array for the reduction of background autofluorescence in brain tissue

Journal of Neuroscience Methods

Volumn 220, Issue 1, 2013, Pages 46-54

  Duong, Haison ^a   Han, Martin ^a  

^a HUNTINGTON MEDICAL RESEARCH INSTITUTES   (United States)

Author keywords

Antibody; Autofluoresence; Fluorophores; Immunofluorescence; Light emitting diodes (LEDs); Photo irradiation

Indexed keywords

COPPER SULFATE; MICROTUBULE ASSOCIATED PROTEIN 2;

ANIMAL TISSUE; ARTICLE; AUTOFLUORESCENCE; BRAIN CORTEX; BRAIN TISSUE; CHEMICAL LABELING; CONJUGATE; COST; EXPERIMENTAL CAT; EXPERIMENTAL RABBIT; FLUORESCENT LIGHTING; HEAT; HISTOLOGY; ILLUMINATION; IMMUNOFLUORESCENCE; IRRADIATION; LIGHT EMITTING DIODE; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; TIME; ULTRAVIOLET RADIATION;

AAFS; ANTIBODY; ARTIFACTUAL AUTOFLUORESCENCES; AUTOFLUORESENCE; FLUOROPHORES; IMMUNOFLUORESCENCE; LEDS; LIGHT EMITTING DIODES; LIGHT EMITTING DIODES (LEDS); PHOTO-IRRADIATION; ULTRAVIOLET; UV;

ANIMALS; ARTIFACTS; BRAIN; CATS; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOHISTOCHEMISTRY; LIGHT; PHOTOBLEACHING; RABBITS;

EID: 84884390044     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2013.08.018     Document Type: Article

References (27)

1. Andersson H., Baechi T., Hoechi M., Richter C. Autofluorescence of living cells. J Microsc 1998, 191:1-7.
2. Barden H. Interference filter microfluorometry of neuromelanin and lipofuscin in human brain. J Neuropathol Exp Neurol 1980, 37:437-451.
3. Beisker W., Dolbeane F., Gray J.W. An improved immunocytochemical procedure for high-sensitivity detection of incorporated bromodeoxyuridine. Cytometry 1987, 8:235-239.
4. Benson R.C., Meyer R.A., Zaruba M.W., McKhann G.M. Cellular autofluorescence: it is due to flavins. J Histochem Cytochem 1979, 27:44-48.
5. Billinton N., Knight A.W. Seeing the wood through the tree: a review of techniques for distinguishing green fluorescence protein from endogenous autofluorescence. Anal Biochem 2001, 291:175-197.
6. Clancy B., Cauller L.J. Reduction of background autofluorescence in brain sections following immersion of sodium borohydride. J Neurosci Methods 1998, 83:97-102.
7. Cowen T., Haven A.J., Burnstock G. Pontamine Sky Blue: a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. Histochemistry 1985, 82:205-208.
8. Diez-Fraile A., Van Hecke N., Guerin C.J., D'Herde K. Optimizing multiple immunostaining of neural tissue. Applications of immunocytochemistry 2012, 29-48. InTech Publishing.
9. Duong H., Pikov V., Han M. Use of a multispectral LED light array for the reduction of autofluorescence in brain tissue 2012, 14. Society for Neuroscience, New Orleans, LA, (Prog. no. 404).
10. Gu Y., Narendran N. Design and evaluation of an LED-based fixture. Third International Conference on Solid State Lighting, Proceedings of SPIE 5187. 2004, 318-329.
11. Han M., Manoonkitiwongsa P.S., Wang C., McCreery D.B. In vivo validation of custom-designed silicon-based microelectrode arrays for long-term neural recording and stimulation. IEEE Trans Biomed Eng 2012, 59(2):346-354.
12. Mueller G. Electroluminescence 1. Semiconductors and semimetals 2000, vol. 64. Academic Press, ISBN 0-12-752173-9.
13. Neumann M., Gabel D. Simple method for reduction of autofluorescence in fluorescence. J Histochem Cytochem 2002, 50(3):437-438.
14. O'Connell M.J., Baum H., Peters T.J. Haemosiderin-like properties of free radical modified ferritin. Biochem J 1986, 240:297-300.
15. Oliveira V.C., Carrara R.C.V., Simoes D.L.C., Saggioro F.P., Carlotti C.G., Covas D.T., et al. Sudan Black B treatment reduces autofluorescence and improves resolution of in situ hybridization specific fluorescent signals of brain sections. Histol Histopathol 2010, 25:1017-1024.
16. Potter K.A., Simon J.S., Velagapudi B., Capadona J.R. Reduction of autofluorescence at the microelectrode-cortical tissue interface improves antibody detection. J Neurosci Methods 2012, 203(1):96-105.
17. Rost F.W.D. Fluorescence microscopy 1995, vol. 2. Cambridge University Press, (chapter 9).
18. Schnell S.A., Staines W.A., Wessendorf M.W. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J Histochem Cytochem. 1999, 47(6):716-730.
19. Schubert E.F. Light-emitting diodes 2006, Cambridge University Press, ISBN 0-521-86538.
20. Seehafer S.S., Pearce D.A. You say lipofuscin, we say ceroid: defining autofluorescent storage material. Neurobiol Aging 2006, 27:576-588.
21. Siakotos A.N., Munkres K.D. Recent developments in the isolation and properties of autofluorescent lipopigments. Ceroid lipofuscinosis 1982, 167-178. D. Armstrong, N. Koppang, J.A. Rider (Eds.).
22. Steinkamp J.A., Stewart C.C. Dual-laser, differential fluorescence correction method for reducing cellular background autofluorescence. Cytometry 1986, 7:566-574.
23. Viegas M.S., Martins T.C., Seco F., do Carmo A. An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalin fixed paraffin-embedded tissues. Eur J Histochem 2007, 51(1):59-66.
24. Van de Lest C.H., Versteeg E.M., Veerkamp J.H., Van Kuppevelt T.H. Elimination of autofluorescence in immunofluorescence microscopy with digital image processing. J Histochem Cytochem 1995, 43:727-730.
25. Wang H., Pevsner J. Detection of endogenous biotin in various tissues: novel functions in the hippocampus and implications for its use in avidin-biotin technology. Cell Tissues Res 1999, 296:511-516.
26. Widengren J., Rigler R. Mechanism of photobleaching investigated by fluorescence correlation spectroscopy. Bioimaging 1996, 4:149-157.
27. Zarkowsky D., Lamoreaux L., Chattopadhyay P., Koup R.A., Perfetto S.P., Roederer M. Heavy metals contaminants can eliminate quantum dot fluorescence. Cytometry A 2011, 79(1):84-89.