ClearT: A detergent- and solvent-free clearing method for neuronal and non-neuronal tissue

Development (Cambridge)

Volumn 140, Issue 6, 2013, Pages 1364-1368

  Kuwajima, Takaaki ^a   Sitko, Austen A ^a   Bhansali, Punita ^a   Jurgens, Chris ^b   Guido, William ^b,c   Mason, Carol ^a  

^a College of Physicians and Surgeons ^*  (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^c University of Louisville School of Medicine   (United States)

Author keywords

Clearing reagent; DiI; Fluorescent protein; Immunohistochemistry; Retinal axon pathway; Whole mount

Indexed keywords

FLUORESCENT DYE; FORMAMIDE; MACROGOL; TRACER; DETERGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; SOLVENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL STRUCTURE; CHEMICAL LABELING; CHEMICAL PROCEDURES; CLEAR T METHOD; CLEAR T2 METHOD; EMBRYO; IMMUNOHISTOCHEMISTRY; LIPOPHILICITY; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; ANIMAL; ANIMAL EMBRYO; BIOLOGICAL MODEL; BRAIN; C57BL MOUSE; CYTOLOGY; DISSECTION; LABORATORY DIAGNOSIS; METHODOLOGY; PHYSIOLOGY; STAINING; TISSUE FIXATION; VALIDATION STUDY;

ANIMALS; BRAIN; CYTOLOGICAL TECHNIQUES; DETERGENTS; DISSECTION; EMBRYO, MAMMALIAN; INDICATORS AND REAGENTS; MICE; MICE, INBRED C57BL; MODELS, BIOLOGICAL; NEURONS; SOLVENTS; SPECIMEN HANDLING; STAINING AND LABELING; TISSUE FIXATION;

MLCS; MLOWN;

EID: 84874536260     PISSN: 09501991     EISSN: 14779129     Source Type: Journal    
DOI: 10.1242/dev.091844     Document Type: Article

References (15)

1. Bielle, F., Marcos-Mondéjar, P., Leyva-Díaz, E., Lokmane, L., Mire, E., Mailhes, C., Keita, M., García, N., Tessier-Lavigne, M., Garel, S. et al. (2011). Emergent growth cone responses to combinations of Slit1 and Netrin 1 in thalamocortical axon topography. Curr. Biol. 21, 1748-1755.
2. Dodt, H. U., Leischner, U., Schierloh, A., Jährling, N., Mauch, C. P., Deininger, K., Deussing, J. M., Eder, M., Zieglgänsberger, W. and Becker, K. (2007). Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain. Nat. Methods 4, 331-336.
3. Ertürk, A., Mauch, C. P., Hellal, F., Förstner, F., Keck, T., Becker, K., Jährling, N., Steffens, H., Richter, M., Hübener, M. et al. (2012). Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury. Nat. Med. 18, 166-171.
4. Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W. and Sanes, J. R. (2000). Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51.
5. Ferrer-Vaquer, A., Piliszek, A., Tian, G., Aho, R. J., Dufort, D. and Hadjantonakis, A. K. (2010). A sensitive and bright single-cell resolution live imaging reporter of Wnt/ß-catenin signaling in the mouse. BMC Dev. Biol. 10, 121.
6. Hama, H., Kurokawa, H., Kawano, H., Ando, R., Shimogori, T., Noda, H., Fukami, K., Sakaue-Sawano, A. and Miyawaki, A. (2011). Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nat. Neurosci. 14, 1481-1488.
7. Huber, A. B., Kania, A., Tran, T. S., Gu, C., De Marco Garcia, N., Lieberam, I., Johnson, D., Jessell, T. M., Ginty, D. D. and Kolodkin, A. L. (2005). Distinct roles for secreted semaphorin signaling in spinal motor axon guidance. Neuron 48, 949-964.
8. Ikawa, M., Kominami, K., Yoshimura, Y., Tanaka, K., Nishimune, Y. and Okabe, M. (1995). A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP). FEBS Lett. 375, 125-128.
9. Jaubert-Miazza, L., Green, E., Lo, F. S., Bui, K., Mills, J. and Guido, W. (2005). Structural and functional composition of the developing retinogeniculate pathway in the mouse. Vis. Neurosci. 22, 661-676.
10. Krahe, T. E., El-Danaf, R. N., Dilger, E. K., Henderson, S. C. and Guido, W. (2011). Morphologically distinct classes of relay cells exhibit regional preferences in the dorsal lateral geniculate nucleus of the mouse. J. Neurosci.31, 17437-17448.
11. Little, G. E., López-Bendito, G., Rünker, A. E., García, N., Piñon, M. C., Chédotal, A., Molnár, Z. and Mitchell, K. J. (2009). Specificity and plasticity of thalamocortical connections in Sema6A mutant mice. PLoS Biol. 7, e98.
12. Luo, L., Callaway, E. M. and Svoboda, K. (2008). Genetic dissection of neural circuits. Neuron 57, 634-660.
13. Plump, A. S., Erskine, L., Sabatier, C., Brose, K., Epstein, C. J., Goodman, C. S., Mason, C. A. and Tessier-Lavigne, M. (2002). Slit1 and Slit2 cooperate to prevent premature midline crossing of retinal axons in the mouse visual system. Neuron 33, 219-232.
14. Rawat, S., Raman Suri, C. and Sahoo, D. K. (2010). Molecular mechanism of polyethylene glycol mediated stabilization of protein. Biochem. Biophys. Res. Commun. 392, 561-566.
15. Rebsam, A., Petros, T. J. and Mason, C. A. (2009). Switching retinogeniculate axon laterality leads to normal targeting but abnormal eye-specific segregation that is activity dependent. J. Neurosci. 29, 14855-14863.