Ultrasonic decalcification offers new perspectives for rapid FISH, DNA, and RT-PCR analysis in bone marrow trephines

American Journal of Surgical Pathology

Volumn 30, Issue 7, 2006, Pages 892-896

  Reineke, Tanja ^a   Jenni, Bettina ^a   Abdou, Marie Therese ^a   Frigerio, Simona ^a   Zubler, Petra ^a   Moch, Holger ^a   Tinguely, Marianne ^a  

^a UNIVERSITY HOSPITAL ZURICH   (Switzerland)

Author keywords

Bone marrow trephine; Decalcification; FISH; Molecular analysis; PCR; Ultrasound

Indexed keywords

DNA; EDETIC ACID; RNA; CALCIUM; FIXATIVE; FORMALDEHYDE; NITRIC ACID; PARAFFIN;

ARTICLE; BONE MARROW BIOPSY; BONE MARROW EXAMINATION; CASE REPORT; DNA SEQUENCE; FLUORESCENCE IN SITU HYBRIDIZATION; HEMATOLOGIC DISEASE; HISTOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE FIXATION; ULTRASOUND; BIOPSY; CHEMISTRY; COMPARATIVE STUDY; LABORATORY DIAGNOSIS; METHODOLOGY;

BIOPSY; BONE MARROW EXAMINATION; CALCIUM; DECALCIFICATION TECHNIQUE; DNA; EDETIC ACID; FIXATIVES; FORMALDEHYDE; IN SITU HYBRIDIZATION, FLUORESCENCE; NITRIC ACID; PARAFFIN EMBEDDING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SPECIMEN HANDLING; TISSUE FIXATION; ULTRASONICS;

EID: 33746893059     PISSN: 01475185     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.pas.0000213282.20166.13     Document Type: Article

References (14)

1. Bock O, Lehmann U, Kreipe H. Quantitative intra-individual monitoring of BCR-ABL transcript levels in archival bone marrow trephines of patients with chronic myeloid leukemia. J Mol Diagn. 2003;5:54-60.
2. Bock O, Reising D, Kreipe H. Multiplex RT-PCR for the detection of common BCR-ABL fusion transcripts in paraffin-embedded tissues from patients with chronic myeloid leukemia and acute lymphoblastic leukemia. Diagn Mol Pathol. 2003;12:119-123.
3. Brown RS, Edwards J, Bartlett JW, et al. Routine acid decalcification of bone marrow samples can preserve DNA for FISH and CGH studies in metastatic prostate cancer. J Histochem Cytochem. 2002;50:113-115.
4. Corrias MV, Faulkner LB, Pistorio A, et al. Detection of neuroblastoma cells in bone marrow and peripheral blood by different techniques: accuracy and relationship with clinical features of patients. Clin Cancer Res. 2004;10:7978-7985.
5. Fend F, Bock O, Kremer M, et al. Ancillary techniques in bone marrow pathology: molecular diagnostics on bone marrow trephine biopsies. Virchows Arch. 2005;447:909-919.
6. Fonseca R, Blood E, Rue M, et al. Clinical and biologic implications of recurrent genomic aberrations in myeloma. Blood. 2003;101:4569-4575.
7. Jaffe E, Harris NL, Stein H, et al. Pathology and Genetics of Tumours of Haematopathopoietic and Lymphoid Tissue. Lyon, France: IARC Press; 2001.
8. Kremer M, Spitzer M, Mandl-Weber S, et al. Discordant bone marrow involvement in diffuse large B-cell lymphoma: comparative molecular analysis reveals a heterogeneous group of disorders. Lab Invest. 2003;83:107-114.
9. Kremer M, Quintanilla-Martinez L, Nahrig J, et al. Immunohistochemistry in bone marrow pathology: a useful adjunct for morphologic diagnosis. Virchows Arch. 2005;447:920-937.
10. Milan L, Trachtenberg MC. Ultrasonic decalcification of bone. Am J Surg Pathol. 1981;5:573-579.
11. Tinguely M, Frigerio S. Assessment of translocations in routine diagnostics of a surgical pathology unit. Ther Umsch. 2006;63:279-285.
12. Wickham CL, Sarsfield P, Joyner MV, et al. Formic acid decalcification of bone marrow trephines degrades DNA: alternative use of EDTA allows the amplification and sequencing of relatively long PCR products. Mol Pathol. 2000;53:336.
13. Yamamoto-Fukud T, Shibata Y, Hishikawa Y, et al. Effects of various decalcification protocols on detection of DNA strand breaks by terminal dUTP nick end labelling. Histochem J. 2000;32:697-702.
14. Zsikla V, Baumann M, Cathomas G. Effect of buffered formalin on amplification of DNA from paraffin wax embedded small biopsies using real-time PCR. J Clin Pathol. 2004;57:654-656.