Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round

Analytical Biochemistry

Volumn 324, Issue 2, 2004, Pages 285-291

  Seyfang, Andreas ^a   Huaqian Jin, Jean ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

Candida albicans dominant selection marker; Codon optimization; Functional proteomics; Genetic engineering; Multiple site directed mutagenesis

Indexed keywords

AMINO ACIDS; CANDIDA; EFFICIENCY; GENETIC ENGINEERING; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEINS; PROTEOMICS; YEAST;

ALANINE-SCANNING MUTAGENESIS; CANDIDA ALBICANS; CODON OPTIMIZATION; HETEROLOGOUS EXPRESSION; MULTIPLE-SITE MUTAGENESIS; SELECTION MARKER; SITE DIRECTED MUTAGENESIS; STRUCTURE-FUNCTION RELATIONSHIP;

SITE SELECTION;

DNA; DNA POLYMERASE; HYGROMYCIN B PHOSPHOTRANSFERASE; KANAMYCIN KINASE; LEUCINE; MUTANT PROTEIN; NUCLEOTIDE; PHOSPHOTRANSFERASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PRIMER DNA; UNCLASSIFIED DRUG;

ARTICLE; CANDIDA ALBICANS; CODON; DNA TEMPLATE; FUNGAL GENE; GENE MUTATION; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SITE DIRECTED MUTAGENESIS;

CANDIDA; CANDIDA ALBICANS;

EID: 0346362261     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.10.012     Document Type: Article

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