A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library

Analytical Biochemistry

Volumn 449, Issue 1, 2014, Pages 90-98

  Jain, Pankaj C ^a   Varadarajan, Raghavan ^a,b  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

Base mismatch; High throughput; PCR bias; Primer design; Site directed mutagenesis

Indexed keywords

GENES; INVERSE PROBLEMS; LIBRARIES; NUCLEOTIDES;

BASE MISMATCH; HIGH THROUGHPUT; PCR BIAS; PRIMER DESIGN; SITE DIRECTED MUTAGENESIS;

POLYMERASE CHAIN REACTION;

NUCLEOTIDE;

ARTICLE; CODON; DELETION MUTANT; INVERSE POLYMERASE CHAIN REACTION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS;

BASE MISMATCH; HIGH THROUGHPUT; PCR BIAS; PRIMER DESIGN; SITE-DIRECTED MUTAGENESIS;

BASE PAIR MISMATCH; BASE SEQUENCE; CODON; DNA PRIMERS; ESCHERICHIA COLI; GENE LIBRARY; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION;

EID: 84892698156     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.12.002     Document Type: Article

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