Random-priming in vitro recombination: An effective tool for directed evolution

Nucleic Acids Research

Volumn 26, Issue 2, 1998, Pages 681-683

  Shao, Zhixin ^a   Zhao, Huimin ^a   Giver, Lori ^a   Arnold, Frances H ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; GENE PRODUCT; POLYNUCLEOTIDE; SUBTILISIN;

ARTICLE; DENATURATION; EVOLUTION; GENETIC RECOMBINATION; MUTAGENESIS; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERIZATION; PRIORITY JOURNAL; SCREENING;

BACILLUS SUBTILIS; DNA; DNA POLYMERASE I; DNA PRIMERS; ENZYME STABILITY; EVOLUTION, MOLECULAR; GENE EXPRESSION; MUTAGENESIS; POINT MUTATION; POLYNUCLEOTIDES; RECOMBINATION, GENETIC; SUBTILISINS; TEMPLATES, GENETIC;

EID: 0032518181     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/26.2.681     Document Type: Article

References (10)

1. Kuchner, O. and Arnold, F.H. (1997) Trends Biotech., 15, 523-530.
2. Stemmer, W.P.C. (1994) Nature, 370, 389-391.
3. Stemmer, W.P.C. (1994) Proc. Natl. Acad. Sci. USA, 91, 10747-10751.
4. Moore, J.C., Jin, H.-M., Kuchner, O. and Arnold, F.H. (1997) J. Mol. Biol., 272, 336-347.
5. Moore, J.C. and Arnold, F.H. (1996) Nature Biotech., 14, 458-467.
6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Feinberg, A.P. and Vogelstein, B. (1983) Anal. Biochem., 132, 6-13.
8. Hodgson, C.P. and Fisk, R.Z. (1987) Nucleic Acids Res., 15, 6296.
9. Zhao, H. and Arnold, F.H. (1997) Proc. Natl. Acad. Sci. USA, 94, 7997-8000.
10. Shafikhani, S., Siegel, R.A., Ferrari, E. and Schellenberger, V. (1997) Biotechniques, 23, 304-310.