Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair

Cell Reports

Volumn 14, Issue 6, 2016, Pages 1555-1566

  Gutschner, Tony ^a   Haemmerle, Monika ^a,b   Genovese, Giannicola ^a   Draetta, Giulio F ^a   Chin, Lynda ^a  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^b UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

Cell cycle; CRISPR; Genome editing; Homologous recombination; MALAT1; Proteolysis; Synthetic biology

Indexed keywords

ENDONUCLEASE; PROTEIN CAS9; UNCLASSIFIED DRUG; APC PROTEIN; APC PROTEIN, HUMAN; BACTERIAL PROTEIN; CADHERIN; CAS9 PROTEIN, FRANCISELLA NOVICIDA; CDH1 PROTEIN, HUMAN; DNA; GEMININ; GMNN PROTEIN, HUMAN; HYBRID PROTEIN; UBIQUITIN PROTEIN LIGASE;

ARTICLE; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE PROGRESSION; CELL CYCLE S PHASE; CONTROLLED STUDY; DNA CONTENT; DNA DAMAGE; DNA REPAIR; EGFP GENE; GENE EXPRESSION; GENE LOCUS; HUMAN; HUMAN CELL; MALAT1 GENE; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; REPORTER GENE; UBIQUITINATION; CHEMISTRY; CRISPR CAS SYSTEM; DNA END JOINING REPAIR; DOUBLE STRANDED DNA BREAK; G1 PHASE CELL CYCLE CHECKPOINT; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; PROCEDURES; RECOMBINATION REPAIR; TRANSGENE;

ADENOMATOUS POLYPOSIS COLI PROTEIN; BACTERIAL PROTEINS; BASE SEQUENCE; CADHERINS; CRISPR-CAS SYSTEMS; DNA; DNA BREAKS, DOUBLE-STRANDED; DNA END-JOINING REPAIR; ENDONUCLEASES; G1 PHASE CELL CYCLE CHECKPOINTS; GEMININ; GENE EXPRESSION REGULATION; GENE KNOCK-IN TECHNIQUES; GENETIC ENGINEERING; HEK293 CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; RECOMBINATIONAL DNA REPAIR; TRANSFECTION; TRANSGENES; UBIQUITIN-PROTEIN LIGASES;

EID: 84958111696     PISSN: None     EISSN: 22111247     Source Type: Journal    
DOI: 10.1016/j.celrep.2016.01.019     Document Type: Article

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